浙江化工
浙江化工
절강화공
ZHEJIANG CHEMICAL INDUSTRY
2015年
4期
51-53
,共3页
羟丙基纤维素填充色谱柱%研制和应用%柱效
羥丙基纖維素填充色譜柱%研製和應用%柱效
간병기섬유소전충색보주%연제화응용%주효
chromatographic column packed with hydroxy propyl cellulose%development and applica-tion%column efficiency
根据2010版药典要求，研制了20%OV-7 i.d.2 mm×0.5 mm×3 m填充色谱柱用于分析羟丙基纤维素中羟丙氧基含量的分析。考察了色谱柱的规格、不同载体对羟丙基纤维素中被测组分的影响。建立了羟丙基纤维素色谱柱制备及样品定性、定量的新方法。试验结果表明，在选定的色谱条件下，各组分的分离度和理论塔板数都远超药典中规定的参数。标准样品的重复性好，理论塔板数(n)，校正因子(RSD),相对标准偏差(RSD%)分别为6,1.41%,0.40%，完全符合药典的检测要求。
根據2010版藥典要求，研製瞭20%OV-7 i.d.2 mm×0.5 mm×3 m填充色譜柱用于分析羥丙基纖維素中羥丙氧基含量的分析。攷察瞭色譜柱的規格、不同載體對羥丙基纖維素中被測組分的影響。建立瞭羥丙基纖維素色譜柱製備及樣品定性、定量的新方法。試驗結果錶明，在選定的色譜條件下，各組分的分離度和理論塔闆數都遠超藥典中規定的參數。標準樣品的重複性好，理論塔闆數(n)，校正因子(RSD),相對標準偏差(RSD%)分彆為6,1.41%,0.40%，完全符閤藥典的檢測要求。
근거2010판약전요구，연제료20%OV-7 i.d.2 mm×0.5 mm×3 m전충색보주용우분석간병기섬유소중간병양기함량적분석。고찰료색보주적규격、불동재체대간병기섬유소중피측조분적영향。건립료간병기섬유소색보주제비급양품정성、정량적신방법。시험결과표명，재선정적색보조건하，각조분적분리도화이론탑판수도원초약전중규정적삼수。표준양품적중복성호，이론탑판수(n)，교정인자(RSD),상대표준편차(RSD%)분별위6,1.41%,0.40%，완전부합약전적검측요구。
According to the P. R. China Pharmacopoeia 2010, 20%OV-7 i.d. 2 mm×0.5 mm×3 m filled chromatographic column was developed for determination of hydroxypropoxy content in hydroxy propyl cellu-lose. The effect of specification of column and carrier on detected composition was inspected and a new method was established for preparation of the column packed with hydroxy propyl cellulose and qualitative as well as quantitative analysis. It is manifested that the resolution and the theoretical stage number are far more superior to the regulations of the Pharmacopoeia. It is also proved to have excellent reproducibility by stan-dard samples. The theoretical stage number (n), the correction factor (RSD) and the relative standard devia-tions(RSD%) are 6, 1.41%, 0.40% respectively, completely meet the testing requirement of the Pharma-copoeia.
