CAJ | 학술논문

研究氮磷质量浓度对藻类生长竞争的影响，对于揭示如何通过控制环境因子促进有益藻类生长繁殖、抑制有害藻类生长繁殖，并利用藻类调节养殖环境和提高水体初级生产力具有重要意义。设置了4个氮磷质量浓度梯度（N 0.18μg·mL-1，P 0.025μg·mL-1；N 0.36μg·mL-1，P 0.050μg·mL-1；N 0.72μg·mL-1，P 0.100μg·mL-1；N 3.60μg·mL-1，P 0.500μg·mL-1），通过测算比生长速率、生长曲线、竞争抑制参数，研究了不同氮磷质量浓度对普通小球藻（Chlorella vulgaris）和鱼腥藻（Anabaenasp. strain PCC）生长和种间竞争的影响。结果表明，在单种培养体系中，普通小球藻和鱼腥藻的最大藻细胞数量均随氮磷质量浓度的增加而增加，最大藻细胞数量分别为198.9×105 cells·mL-1和424.8×105 cells·mL-1；氮磷质量浓度对藻类的竞争能够产生明显影响，在共培养体系中，鱼腥藻的最大藻细胞数量表现为：中高氮磷组（208.9×105 cells·mL-1）>中低氮磷组（98.3×105 cells·mL-1）>高氮磷组（64.7×105 cells·mL-1）>低氮磷组（45.3×105 cells·mL-1）。同时，种间竞争抑制参数的测算结果表明，4组氮磷质量浓度下鱼腥藻对普通小球藻的竞争抑制参数（α）分别为2.599、0.564、0.772、1.618，普通小球藻对鱼腥藻的竞争抑制参数（β）分别为0.434、0.321、0.466、-8.899，鱼腥藻对普通小球藻的竞争抑制参数（α）均大于普通小球藻对鱼腥藻的竞争抑制参数（β）；低氮磷质量浓度时，鱼腥藻对普通小球藻的竞争抑制参数（α）最大，为2.599；中高氮磷质量浓度时，普通小球藻对鱼腥藻的竞争抑制参数（β）最大，为0.466。根据Lotka-Volterra竞争模型中的两物种竞争结局可初步判断，低、中高氮磷、高氮磷质量浓度时，鱼腥藻在竞争中占优势；中低氮磷质量浓度时，鱼腥藻和普通小球藻稳定共存。研究氮燐質量濃度對藻類生長競爭的影響，對于揭示如何通過控製環境因子促進有益藻類生長繁殖、抑製有害藻類生長繁殖，併利用藻類調節養殖環境和提高水體初級生產力具有重要意義。設置瞭4箇氮燐質量濃度梯度（N 0.18μg·mL-1，P 0.025μg·mL-1；N 0.36μg·mL-1，P 0.050μg·mL-1；N 0.72μg·mL-1，P 0.100μg·mL-1；N 3.60μg·mL-1，P 0.500μg·mL-1），通過測算比生長速率、生長麯線、競爭抑製參數，研究瞭不同氮燐質量濃度對普通小毬藻（Chlorella vulgaris）和魚腥藻（Anabaenasp. strain PCC）生長和種間競爭的影響。結果錶明，在單種培養體繫中，普通小毬藻和魚腥藻的最大藻細胞數量均隨氮燐質量濃度的增加而增加，最大藻細胞數量分彆為198.9×105 cells·mL-1和424.8×105 cells·mL-1；氮燐質量濃度對藻類的競爭能夠產生明顯影響，在共培養體繫中，魚腥藻的最大藻細胞數量錶現為：中高氮燐組（208.9×105 cells·mL-1）>中低氮燐組（98.3×105 cells·mL-1）>高氮燐組（64.7×105 cells·mL-1）>低氮燐組（45.3×105 cells·mL-1）。同時，種間競爭抑製參數的測算結果錶明，4組氮燐質量濃度下魚腥藻對普通小毬藻的競爭抑製參數（α）分彆為2.599、0.564、0.772、1.618，普通小毬藻對魚腥藻的競爭抑製參數（β）分彆為0.434、0.321、0.466、-8.899，魚腥藻對普通小毬藻的競爭抑製參數（α）均大于普通小毬藻對魚腥藻的競爭抑製參數（β）；低氮燐質量濃度時，魚腥藻對普通小毬藻的競爭抑製參數（α）最大，為2.599；中高氮燐質量濃度時，普通小毬藻對魚腥藻的競爭抑製參數（β）最大，為0.466。根據Lotka-Volterra競爭模型中的兩物種競爭結跼可初步判斷，低、中高氮燐、高氮燐質量濃度時，魚腥藻在競爭中佔優勢；中低氮燐質量濃度時，魚腥藻和普通小毬藻穩定共存。
연구담린질량농도대조류생장경쟁적영향，대우게시여하통과공제배경인자촉진유익조류생장번식、억제유해조류생장번식，병이용조류조절양식배경화제고수체초급생산력구유중요의의。설치료4개담린질량농도제도（N 0.18μg·mL-1，P 0.025μg·mL-1；N 0.36μg·mL-1，P 0.050μg·mL-1；N 0.72μg·mL-1，P 0.100μg·mL-1；N 3.60μg·mL-1，P 0.500μg·mL-1），통과측산비생장속솔、생장곡선、경쟁억제삼수，연구료불동담린질량농도대보통소구조（Chlorella vulgaris）화어성조（Anabaenasp. strain PCC）생장화충간경쟁적영향。결과표명，재단충배양체계중，보통소구조화어성조적최대조세포수량균수담린질량농도적증가이증가，최대조세포수량분별위198.9×105 cells·mL-1화424.8×105 cells·mL-1；담린질량농도대조류적경쟁능구산생명현영향，재공배양체계중，어성조적최대조세포수량표현위：중고담린조（208.9×105 cells·mL-1）>중저담린조（98.3×105 cells·mL-1）>고담린조（64.7×105 cells·mL-1）>저담린조（45.3×105 cells·mL-1）。동시，충간경쟁억제삼수적측산결과표명，4조담린질량농도하어성조대보통소구조적경쟁억제삼수（α）분별위2.599、0.564、0.772、1.618，보통소구조대어성조적경쟁억제삼수（β）분별위0.434、0.321、0.466、-8.899，어성조대보통소구조적경쟁억제삼수（α）균대우보통소구조대어성조적경쟁억제삼수（β）；저담린질량농도시，어성조대보통소구조적경쟁억제삼수（α）최대，위2.599；중고담린질량농도시，보통소구조대어성조적경쟁억제삼수（β）최대，위0.466。근거Lotka-Volterra경쟁모형중적량물충경쟁결국가초보판단，저、중고담린、고담린질량농도시，어성조재경쟁중점우세；중저담린질량농도시，어성조화보통소구조은정공존。
Chlorella vulgaris andAnabaenasp are the most common algae in eutrophication ponds. In order to know the growth process of the two species of algae in eutrophication ponds and the relationship between algae growth and concentrations of nitrogen and phosphorus, the experiment was carried out to research the interspecies competition betweenChlorella vulgaris andAnabaenasp at different nitrogen and phosphorus concentrations (N 0.18 μg·mL-1, P 0.025 μg·mL-1; N 0.36 μg·mL-1, P 0.050 μg·mL-1; N 0.72 μg·mL-1, P 0.100 μg·mL-1; N 3.60 μg·mL-1, P 0.500 μg·mL-1) by the methods of special growth rate, growth curve and inhibition parameters through indoors experiment. For the experiment could help clarify how to promote the growth of useful algae and restrain the growth of harmful algae by the way of regulating the environment factors, so the study is very important for regulating aquaculture eco-environment and improving primary productivity of water body. The results indicated that maximum biomass of bothChlorella vulgaris andAnabaenasp increased with the increase of nitrogen and phosphorus concentrations in the uni-culture system, and the maximum biomass ofChlorella vulgaris andAnabaenasp were 198.9×105 and 424.8×105cells·mL-1 respectively. Nitrogen and phosphorus concentrations could influence the competition between Chlorella vulgaris andAnabaenasp significantly. The maximum biomasses ofAnabaenasp in co-culture system were as follows: (TN 0.72 μg·mL-1, TP 0.100 μg·mL-1) group> (TN 0.36 μg·mL-1, TP 0.050 μg·mL-1) group> (TN 3.60 μg·mL-1, TP 0.500 μg·mL-1) group> (TN 0.18 μg·mL-1, TP 0.025 μg·mL-1) group, and maximum biomasses were 208.9×105, 98.3×105, 64.7×105, 45.3×105cells·mL-1 respectively. The results of inhibition parameter of interspecies competition showed that the inhibition parameters ofAnabaenasp againstChlorella vulgaris were 2.599, 0.564, 0.772, 1.618 respectively and that ofChlorella vulgarisagainst Anabaenasp were 0.434, 0.321, 0.466, -8.899 respectively, that is to say, the inhibition parameters ofAnabaenasp againstChlorella vulgaris were all larger than that ofChlorella vulgarisagainst Anabaenasp at the experiment conditions. The inhibition parameter ofAnabaenasp againstChlorella vulgaris reached the peak at the (TN 0.18 μg·mL-1, TP 0.025 μg·mL-1) group, and the maximum was 2.599. The inhibition parameter of Chlorella vulgaris againstAnabaenasp reached the peak at the (TN 0.72 μg·mL-1, TP 0.100 μg·mL-1) group, and the maximum was 0.466.Anabaenaspdominated in the (TN 0.18 μg·mL-1, TP 0.025 μg·mL-1), (TN 0.72 μg·mL-1, TP 0.100 μg·mL-1) and (TN 3.60 μg·mL-1, TP 0.500 μg·mL-1) groups, and Chlorella vulgaris andAnabaenasp could stably coexist in the (TN 0.36 μg·mL-1, TP 0.050 μg·mL-1) group based on the competition model of Lotka-Volterra.