CAJ | 학술논문

目的：探讨姜黄素和茶多酚对不同时间 UVB 致无毛鼠急性光损伤的防御作用。方法取清洁级无毛鼠36只，随机分为对照组及药物组（0 s、30 s、60 s、120 s、240 s）；每组各2只。于照射前30 min 在无毛鼠背部涂抹姜黄素或茶多酚，照射距离为15 cm，采用3倍最小红斑量（MED）的 UVB 约200～540 mJ／cm 2分别照射各组，取皮肤组织，制成石蜡切片，光学显微镜观察。结果对照组无毛鼠皮肤出现不同程度鳞屑，胶原间可见散在淋巴细胞，且120 s 及240 s 时出现胶原红染、排列紊乱、均质化等改变。药物组无毛鼠真皮中炎性细胞浸润减少，胶原受损现象得到改善。结论外用姜黄素和茶多酚可通过减轻炎症细胞浸润及减轻胶原受损程度而防御 UVB 致无毛鼠皮肤急性光损伤。
목적：탐토강황소화다다분대불동시간 UVB 치무모서급성광손상적방어작용。방법취청길급무모서36지，수궤분위대조조급약물조（0 s、30 s、60 s、120 s、240 s）；매조각2지。우조사전30 min 재무모서배부도말강황소혹다다분，조사거리위15 cm，채용3배최소홍반량（MED）적 UVB 약200～540 mJ／cm 2분별조사각조，취피부조직，제성석사절편，광학현미경관찰。결과대조조무모서피부출현불동정도린설，효원간가견산재림파세포，차120 s 급240 s 시출현효원홍염、배렬문란、균질화등개변。약물조무모서진피중염성세포침윤감소，효원수손현상득도개선。결론외용강황소화다다분가통과감경염증세포침윤급감경효원수손정도이방어 UVB 치무모서피부급성광손상。
Objective To assess the protective of Curcumin and Tea Polyphenols against different time of UVB-induced acute photo damage in hairless mice skin.Methods Thirty six BALB/C hair1ess mice were randomly divided into control groups and treatment groups(all of them were divided into 0 s, 30 s, 60 s, 120 s, 240 s groups with two hairless mice).Curcumin and Tea Polyphenols were applied to the mice’s skin before 30min irradiation.The irradiation distance was 15cm.The mice in each group were given three times of UVB MED about 200 ~540 mJ/cm 2 and the irradiation time were 30 s、60 s、120 s、240 s.After irradiation, the skin on the back of the radiation center were taken into paraffin section for light microscopy observation.Results Different amount of squamae appeared in the control group of hairless rat, and lymphocytes scattered in collagen tissues.In the 120 s and 240 s UVB irradiated group, the collagen was red stained, disorderly arranged, even homogenized.In the treatment group, inflammatory cell infiltration was lessened, and collagen damage weakened.Conclusion By weakening inflammatory cell infiltration and collagen damage, topical application of Curcumin and Tea Polyphenols can protect hairless mice from acute photodamage caused by UVB irradiation.