CAJ | 학술논문

目的：通过检测 ETFβ在糖尿病肾病中的改变，探讨其与脂毒性的关系。方法体内实验采用腹腔注射链脲佐菌素合并单侧肾切除建立糖尿病肾病模型，并评价肾小管损伤情况，检测 ETFβ在肾皮质中的表达变化。体外建立脂肪酸诱导肾小管细胞 NRK 52E 凋亡模型，构建 ETFβ重组质粒，并将其转染至 NRK 52E 细胞，观察 ETFβ过表达对脂肪酸诱导细胞凋亡的作用。结果链脲佐菌素合并单侧肾切除诱导的糖尿病肾病大鼠模型中，肾小管明显损伤，ETFβmRNA 和蛋白表达均下降。脂肪酸可诱导 NRK 52E 细胞凋亡，过表达 ETFβ可减少凋亡。结论糖尿病肾病模型中 ETFβ表达下降，过表达 ETFβ可降低脂肪酸诱导的肾小管细胞凋亡。
목적：통과검측 ETFβ재당뇨병신병중적개변，탐토기여지독성적관계。방법체내실험채용복강주사련뇨좌균소합병단측신절제건립당뇨병신병모형，병평개신소관손상정황，검측 ETFβ재신피질중적표체변화。체외건립지방산유도신소관세포 NRK 52E 조망모형，구건 ETFβ중조질립，병장기전염지 NRK 52E 세포，관찰 ETFβ과표체대지방산유도세포조망적작용。결과련뇨좌균소합병단측신절제유도적당뇨병신병대서모형중，신소관명현손상，ETFβmRNA 화단백표체균하강。지방산가유도 NRK 52E 세포조망，과표체 ETFβ가감소조망。결론당뇨병신병모형중 ETFβ표체하강，과표체 ETFβ가강저지방산유도적신소관세포조망。
Objective To detect the expression change of ETFβin diabetic nephropathy rats and study the role of ETFβin fatty acid-induced apoptosis in renal tubules.Methods Diabetic nephropathy model was established by intraperitoneal injection of streptozotocin and unilateral nephrectomy.In vivo ETFβexpression was detected in renal cortex, as well as tubular injury evaluated.In vitro fatty acid-induced apoptosis in renal tubular cells NRK 52E model was established and ETFβrecombinant plasmid was constructed to be transfected into NRK 52E cells and furtherly to observe the effect of ETFβover-expression on the fatty acid-induced apoptosis.Results In the rats model of diabetic nephropathy induced by streptozotocin injection and unilateral nephrectomy, ETFβmRNA and protein expression were decreased as obvious tubular damage occurred.Fatty acids could induce apoptosis in NRK 52E, and ETFβover-expression reduced the apoptosis.Conclusion The expression of ETFβis decreased in diabetic nephropathy model , and ETFβover-expression can reduce apoptosis induced by fatty acid in renal tubular cells.