CAJ | 학술논문

目的：探讨慢性肠道炎症状态下菌群结构及构成改变对肝癌进展的影响。方法22只雄性乙肝病毒转基因小鼠在出生后第2周腹腔注射二乙基亚硝胺诱导小鼠原发性肝癌，随机分为慢性肠炎组（给予含2％葡聚糖硫酸钠的饮用水）和对照组（给予正常饮用水）。上述小鼠观察至22周龄。在此期间定期进行肝功能测定，收集粪便进行宏基因组测序。另将4只已发生肝癌的小鼠与7只8周龄肉眼及病理学未见肝癌的小鼠同笼（分2笼）饲养16周。结果 DSS 所诱导的慢性肠炎小鼠与正常饮用水小鼠相比肝功能无显著改变；DSS 诱导的慢性肠炎组小鼠（n ＝10）在第22周龄时有9只发生弥漫性肉眼可见的肝癌，而正常饮用水小鼠（n ＝12）仅有2只发生肝癌，肿瘤发生率（P ＝0．002）和肿瘤数量（P ＝0．028）有显著差别；与正常饮用水小鼠相比，慢性肠炎组小鼠肠道菌群中，普雷沃氏菌属（P ＝0．022）和厌氧支原体属（P ＝0．014）显著减少；与已发生肝癌小鼠进行同笼饲养的小鼠（n＝7）在24周龄时全部发生肉眼可见的肝癌。结论慢性肠道炎症状态下，肠道菌群改变可促进肝癌进展。
목적：탐토만성장도염증상태하균군결구급구성개변대간암진전적영향。방법22지웅성을간병독전기인소서재출생후제2주복강주사이을기아초알유도소서원발성간암，수궤분위만성장염조（급여함2％포취당류산납적음용수）화대조조（급여정상음용수）。상술소서관찰지22주령。재차기간정기진행간공능측정，수집분편진행굉기인조측서。령장4지이발생간암적소서여7지8주령육안급병이학미견간암적소서동롱（분2롱）사양16주。결과 DSS 소유도적만성장염소서여정상음용수소서상비간공능무현저개변；DSS 유도적만성장염조소서（n ＝10）재제22주령시유9지발생미만성육안가견적간암，이정상음용수소서（n ＝12）부유2지발생간암，종류발생솔（P ＝0．002）화종류수량（P ＝0．028）유현저차별；여정상음용수소서상비，만성장염조소서장도균군중，보뢰옥씨균속（P ＝0．022）화염양지원체속（P ＝0．014）현저감소；여이발생간암소서진행동롱사양적소서（n＝7）재24주령시전부발생육안가견적간암。결론만성장도염증상태하，장도균군개변가촉진간암진전。
Objective To investigate the effect of gut bacteria under chronic colitis on the progression of hepatoma in mice.Methods 22 hepatitis B virus (HBV) -transgenic mice ( male, 8 weeks) were randomly divided into two groups, one group (n =10) was fed the drinking water containing 2% dextran sodium sulphate(DSS)to induce chronic colitis and the control group(n =12)was fed with normal drinking water.In order to investigate the effect of gut microbes, 7 male HBV-transgenic mice(8 weeks, with no detectable hepatoma under microscopy) were cohoused with 4 mice with hepatoma for 16 weeks.Results No significant liver cell damage was observed in the group of the mice fed with 2% DSS-containing drinking water.By the 22 -week old,9 of the 10 mice(90.0%) fed with 2% DSS-containing drinking water, 2 of the 12 mice(16.7%) fed with normal drinking had hepatoma.Both the hepatoma incidence and the tumor numbers in the group of mice fed with DSS-containing water were significantly higher than that in the controls (P =0.002 and P =0.028respetively).Compared to controls, the bacteria family Prevotella (P =0.022) and Anaeroplasma (P =0.014) reduced significantly in the mice with induced chronic colitis.All the mice (n =7) cohoused with the mice with hepatoma had the liver tumor developed at 24 -week-old.Conclusion Alterations of gut bacteria under chronic colitis may promote the development of liver cancer.