CAJ | 학술논문

目的：评价聚合酶链反应（PCR）快速检测耐甲氧西林金黄色葡萄球菌（MRSA）方法在痰标本中的应用。方法同时采集痰标本送细菌培养、真菌培养和 PCR快速检测，以细菌培养方法为金标准，评价 PCR方法的灵敏度和特异度。结果PCR方法检测痰标本M RSA的灵敏度为88．9％，特异度为73．6％，阳性预测值为36．4％，阴性预测值为97．5％，均较文献报道的鼻拭子PCR的灵敏度和特异度低。结论将痰标本 PCR检测 M RSA用于临床动态监测 M RSA的流行尚缺乏进一步的数据支持，但 PCR结果不受其他细菌生长的影响，而且阴性预测值高达97．5％，在排除 M RSA感染或定植方面有一定价值。
목적：평개취합매련반응（PCR）쾌속검측내갑양서림금황색포도구균（MRSA）방법재담표본중적응용。방법동시채집담표본송세균배양、진균배양화 PCR쾌속검측，이세균배양방법위금표준，평개 PCR방법적령민도화특이도。결과PCR방법검측담표본M RSA적령민도위88．9％，특이도위73．6％，양성예측치위36．4％，음성예측치위97．5％，균교문헌보도적비식자PCR적령민도화특이도저。결론장담표본 PCR검측 M RSA용우림상동태감측 M RSA적류행상결핍진일보적수거지지，단 PCR결과불수기타세균생장적영향，이차음성예측치고체97．5％，재배제 M RSA감염혹정식방면유일정개치。
Objective To evaluate the performance of polymerase chain reaction (PCR)‐based rapid detection of methicillin‐resistant S .aureus (MRSA) from the respiratory specimens of ICU patients in comparison with conventional sputum culture . Methods We collected 3 respiratory specimens from each patient simultaneously for bacterial culture ,fungal culture and PCR‐based detection of MRSA .The performance of the established PCR method was compared with the results of sputum culture in terms of sensitivity ,specificity ,positive predictive value (PPV) and negative predictive value (NPV) .Results The sensitivity , specificity ,PPV and NPV of PCR assay were 88 .9% ,73 .6% ,36 .4% ,and 97 .5% ,respectively ,which were lower than the reported results of PCR assay with nasal swabs .Conclusions The utility of PCR assay for active screening of MRSA from respiratory specimens still needs further supportive data .However ,PCR assay is somewhat informative in excluding MRSA infection or colonization because of its high NPV .