CAJ | 학술논문

目的：分析产K PC酶肺炎克雷伯菌的耐药性和基因型及对替加环素的敏感性，为临床治疗该类菌感染提供依据。方法收集2012年2月—2013年5月自临床科室分离的碳青霉烯类耐药肺炎克雷伯菌9株，采用VITEK 2 Compact全自动微生物鉴定系统进行细菌鉴定及药敏试验，改良Hodge试验检测碳青霉烯酶，聚合酶链反应（PCR）法检测菌株的KPC酶基因型，E试验法检测替加环素对菌株的体外抗菌活性。结果所分离的9株肺炎克雷伯菌对所测试的19种抗菌药物均显耐药，7株改良Hodge试验阳性。9株肺炎克雷伯菌均扩增出KPC目的基因条带，均为KPC‐2型基因。9株耐药菌对替加环素均敏感。结论产K PC酶肺炎克雷伯菌耐药形势严峻，替加环素作为新一代抗生素对其有良好的体外抗菌活性。
목적：분석산K PC매폐염극뢰백균적내약성화기인형급대체가배소적민감성，위림상치료해류균감염제공의거。방법수집2012년2월—2013년5월자림상과실분리적탄청매희류내약폐염극뢰백균9주，채용VITEK 2 Compact전자동미생물감정계통진행세균감정급약민시험，개량Hodge시험검측탄청매희매，취합매련반응（PCR）법검측균주적KPC매기인형，E시험법검측체가배소대균주적체외항균활성。결과소분리적9주폐염극뢰백균대소측시적19충항균약물균현내약，7주개량Hodge시험양성。9주폐염극뢰백균균확증출KPC목적기인조대，균위KPC‐2형기인。9주내약균대체가배소균민감。결론산K PC매폐염극뢰백균내약형세엄준，체가배소작위신일대항생소대기유량호적체외항균활성。
Objective To analyze the resistant profile and genotypes ,as well as susceptibility to tigecycline in carbapenemase‐producing K lebsiella pneumoniae for better clinical management of such infections .Methods Nine strains of carbapenemase‐producing K .pneumoniae were isolated from clinical specimens during the period from February 2012 to May 2013 in our hospital .Bacterial identification and antimicrobial susceptibility testing were carried out with VITEK 2 Compact automatic microbiological assay systems .The phenotype of carbapenemase‐producing K . pneumoniae was detected by modified Hodges test .The genotype of carbapenemase was identified by PCR method .The susceptibility to tigecycline was tested by E‐test . Results All the 9 strains of carbapenemase‐producing K . pneumoniae were resistant to the 19 antibiotics tested .Modified Hodges test was positive for 7 strains (77 .8% ) .Target band of carbapenemase gene was identified in all the 9 strains of K . pneumoniae ,and all were confirmed as KPC‐2 gene .All the 9 strains were susceptible to tigecycline .Conclusions The resistance of carbapenemase‐producing K .pneumoniae is still serious .Tigecycline has shown good in vitro activity against such strains .