临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2015年
5期
552-555
,共4页
郭忠英%傅广波%刘海燕%孙苏安
郭忠英%傅廣波%劉海燕%孫囌安
곽충영%부엄파%류해연%손소안
前列腺肿瘤%活性氧%p70S6K1%肿瘤新生血管生成
前列腺腫瘤%活性氧%p70S6K1%腫瘤新生血管生成
전렬선종류%활성양%p70S6K1%종류신생혈관생성
prostate neoplasm%reactive oxygen species%p70S6K1%tumor angiogenesis
目的：检测前列腺癌(prostatic cancer, PCa)和癌旁组织中活性氧(reactive oxygen species, ROS)的表达、PI3K/AKT信号通路中相关基因蛋白p70S6K1、VEGF的表达及微血管密度( micro-vascular density, MVD)值,分析ROS与p70S6K1、VEGF蛋白之间的相关性,以及ROS、p70S6K1、VEGF蛋白表达与PCa血管生成的关系。方法采用免疫荧光法检测PCa和癌旁组织内的ROS水平；免疫组化SP法检测PI3K/AKT通路相关基因蛋白p70S6K1、VEGF表达及MVD计数情况。结果 PCa组织中ROS的表达水平明显高于癌旁组织,差异有统计学意义(P<0．05)；PCa组织中p70S6K1、VEGF蛋白表达及MVD值高于癌旁组织,差异有统计学意义(P<0．05),PCa组织中ROS、p70S6K1、VEGF三者表达间均呈正相关,且ROS、p70S6K1及VEGF表达均与MVD值呈正相关。结论(1)PCa组织中ROS表达水平明显高于癌旁组织。(2)PCa组织中PI3K/AKT通路相关基因蛋白p70S6K1、VEGF蛋白表达高于癌旁组织,PCa中MVD值高于癌旁组织,提示PI3K/AKT信号通路在肿瘤血管生成中起一定作用。(3)PCa组织中ROS表达水平和PI3K/AKT通路相关基因蛋白p70S6K1、VEGF表达及MVD值均呈正相关,提示ROS可能通过PI3K/AKT信号通路,对PCa发生、发展起正调控作用。
目的：檢測前列腺癌(prostatic cancer, PCa)和癌徬組織中活性氧(reactive oxygen species, ROS)的錶達、PI3K/AKT信號通路中相關基因蛋白p70S6K1、VEGF的錶達及微血管密度( micro-vascular density, MVD)值,分析ROS與p70S6K1、VEGF蛋白之間的相關性,以及ROS、p70S6K1、VEGF蛋白錶達與PCa血管生成的關繫。方法採用免疫熒光法檢測PCa和癌徬組織內的ROS水平；免疫組化SP法檢測PI3K/AKT通路相關基因蛋白p70S6K1、VEGF錶達及MVD計數情況。結果 PCa組織中ROS的錶達水平明顯高于癌徬組織,差異有統計學意義(P<0．05)；PCa組織中p70S6K1、VEGF蛋白錶達及MVD值高于癌徬組織,差異有統計學意義(P<0．05),PCa組織中ROS、p70S6K1、VEGF三者錶達間均呈正相關,且ROS、p70S6K1及VEGF錶達均與MVD值呈正相關。結論(1)PCa組織中ROS錶達水平明顯高于癌徬組織。(2)PCa組織中PI3K/AKT通路相關基因蛋白p70S6K1、VEGF蛋白錶達高于癌徬組織,PCa中MVD值高于癌徬組織,提示PI3K/AKT信號通路在腫瘤血管生成中起一定作用。(3)PCa組織中ROS錶達水平和PI3K/AKT通路相關基因蛋白p70S6K1、VEGF錶達及MVD值均呈正相關,提示ROS可能通過PI3K/AKT信號通路,對PCa髮生、髮展起正調控作用。
목적：검측전렬선암(prostatic cancer, PCa)화암방조직중활성양(reactive oxygen species, ROS)적표체、PI3K/AKT신호통로중상관기인단백p70S6K1、VEGF적표체급미혈관밀도( micro-vascular density, MVD)치,분석ROS여p70S6K1、VEGF단백지간적상관성,이급ROS、p70S6K1、VEGF단백표체여PCa혈관생성적관계。방법채용면역형광법검측PCa화암방조직내적ROS수평；면역조화SP법검측PI3K/AKT통로상관기인단백p70S6K1、VEGF표체급MVD계수정황。결과 PCa조직중ROS적표체수평명현고우암방조직,차이유통계학의의(P<0．05)；PCa조직중p70S6K1、VEGF단백표체급MVD치고우암방조직,차이유통계학의의(P<0．05),PCa조직중ROS、p70S6K1、VEGF삼자표체간균정정상관,차ROS、p70S6K1급VEGF표체균여MVD치정정상관。결론(1)PCa조직중ROS표체수평명현고우암방조직。(2)PCa조직중PI3K/AKT통로상관기인단백p70S6K1、VEGF단백표체고우암방조직,PCa중MVD치고우암방조직,제시PI3K/AKT신호통로재종류혈관생성중기일정작용。(3)PCa조직중ROS표체수평화PI3K/AKT통로상관기인단백p70S6K1、VEGF표체급MVD치균정정상관,제시ROS가능통과PI3K/AKT신호통로,대PCa발생、발전기정조공작용。
Purpose To investigate the expression of reactive oxygen species (ROS) and PI3K/AKT signaling pathway associated gene protein p70S6K1, VEGF in prostate cancer and adjacent tissues, and to determine their relationship with micro-vascular density ( MVD) , as well as the relationship between themselves. Methods The expression of ROS was detected by immunofluorescence, and the expression of p70S6K1, VEGF and MVD counting were detected by immunohistochemistry. Results Compared to adjacent tis-sues, the expression of ROS, p70S6K1, VEGF and MVD in PCa were increased considerablely (P<0. 05). In prostate cancer the ex-pression of ROS were positively correlated to the expression of p70S6K1, VEGF protein, and the expression of ROS and p70S6K1, VEGF were positively correlated to the number of MVD. Conclusion (1)The expression level of ROS in prostate cancer tissues was significantly higher than adjacent tissues. (2) The expression of PI3K/AKT pathway associated gene protein p70S6K1, VEGF protein and MVD counting in prostate cancer tissue was higher than the adjacent tissues, suggesting PI3K/AKT signaling pathways may play a role in tumor angiogenesis. (3) The expression of ROS and PI3K/AKT pathway associated gene protein p70S6K1, VEGF and MVD counting in prostate cancer tissues was positively correlated, suggesting that ROS may play a positive regulatory role for the development of prostate cancer by PI3K/AKT signaling pathway.
