CAJ | 학술논문

目的：探讨子宫颈鳞状细胞癌( cervical squamous cell carcinoma, CSCC)中Aurora-A激酶、微小染色体维持蛋白7( mini-chromosome maintenance protein 7, MCM7)和人乳头瘤病毒16型E7蛋白(human papillomavirus type 16 E7 protein, HPV 16 E7)的表达及与临床病理特征的关系。方法应用免疫组化PV 9001两步法检测子宫颈上皮内瘤变( cervical intraepithelial neopla-sia, CIN)( CIN1者20例、CIN2+3者30例)、40例子宫颈鳞状细胞癌( cervical squamous cell carcinoma, CSCC)及20例慢性子宫颈炎组织中Aurora-A、MCM7及HPV 16 E7的表达,并进行定位、半定量及相互关系的分析。结果(1) Aurora-A定位于细胞质和细胞核,MCM7蛋白阳性染色定位于细胞核,HPV 16 E7以细胞核和(或)细胞质出现棕黄色颗粒为阳性。(2) Aurora-A、MCM7及HPV 16 E7在CSCC组、CIN 2+3组的表达分别高于CIN1组、慢性子宫颈炎组(P<0．0083)。(3)子宫颈浸润癌中,Aurora-A与HPV 16 E7的表达呈正相关(P<0．001,rs =0．657),MCM7与HPV 16 E7的表达呈正相关(P<0．001,rs =0．616),Aurora-A与MCM7的表达呈正相关(P<0．001,rs =0．597)。(4)Aurora-A表达与肿瘤分化程度、临床分期及有无淋巴结转移相关(P<0．05)；MCM7表达与肿瘤分化程度、临床分期相关(P<0．05)；HPV 16 E7表达与患者年龄、肿瘤直径、肿瘤分化程度、临床分期及有无淋巴结转移无相关性(P>0．05)。结论 Aurora-A、MCM7及HPV 16 E7的表达随子宫颈病变进展逐渐增加,三者与子宫颈癌发生、发展密切相关,有望成为子宫颈癌早期诊断、早期治疗的生物学指标。
목적：탐토자궁경린상세포암( cervical squamous cell carcinoma, CSCC)중Aurora-A격매、미소염색체유지단백7( mini-chromosome maintenance protein 7, MCM7)화인유두류병독16형E7단백(human papillomavirus type 16 E7 protein, HPV 16 E7)적표체급여림상병리특정적관계。방법응용면역조화PV 9001량보법검측자궁경상피내류변( cervical intraepithelial neopla-sia, CIN)( CIN1자20례、CIN2+3자30례)、40례자궁경린상세포암( cervical squamous cell carcinoma, CSCC)급20례만성자궁경염조직중Aurora-A、MCM7급HPV 16 E7적표체,병진행정위、반정량급상호관계적분석。결과(1) Aurora-A정위우세포질화세포핵,MCM7단백양성염색정위우세포핵,HPV 16 E7이세포핵화(혹)세포질출현종황색과립위양성。(2) Aurora-A、MCM7급HPV 16 E7재CSCC조、CIN 2+3조적표체분별고우CIN1조、만성자궁경염조(P<0．0083)。(3)자궁경침윤암중,Aurora-A여HPV 16 E7적표체정정상관(P<0．001,rs =0．657),MCM7여HPV 16 E7적표체정정상관(P<0．001,rs =0．616),Aurora-A여MCM7적표체정정상관(P<0．001,rs =0．597)。(4)Aurora-A표체여종류분화정도、림상분기급유무림파결전이상관(P<0．05)；MCM7표체여종류분화정도、림상분기상관(P<0．05)；HPV 16 E7표체여환자년령、종류직경、종류분화정도、림상분기급유무림파결전이무상관성(P>0．05)。결론 Aurora-A、MCM7급HPV 16 E7적표체수자궁경병변진전축점증가,삼자여자궁경암발생、발전밀절상관,유망성위자궁경암조기진단、조기치료적생물학지표。
Purpose To explore the expression of Aurora kinase A (Aurora-A), minichromosome maintenance protein 7 (MCM7) and human papillomavirus type 16 E7 protein (HPV 16 E7) in uterine cervical squamous cell carcinoma (CSCC) and to investigate their relationship with clinicopathological factors. Methods Immunohistochemical method was employed on 20 cases of low-grade cervical intraepithelial neoplasia (CIN1) , 30 cases of high-grade cervical intraepithelial neoplasia (CIN2+3), 40 cases of CSCC, and 20 ca-ses of chronic cervicitis. Results (1) Aurora-A localized in the cytoplasm and nucleus. MCM7 protein positive staining localized in the nucleus. In the nucleus, and (or) the cytoplasm appeared brown particles positive for HPV 16 E7. (2) The expression of Aurora-A, MCM7 and HPV 16 E7 were higher in the group of CIN2+3 and CSCC than that in the group of chronic cervicitis or CIN1 ( P<0. 0083). (3) In cervical cancer, the expression of Aurora-A, HPV 16 E7 showed positive correlation (P<0. 001, rs =0. 657). The expression of MCM7, HPV 16 E7 showed positive correlation (P<0. 001, rs =0. 616). The expression of Aurora-A, MCM7 showed positive correlation (P<0. 001, rs =0. 597). (4) Aurora-A expression levels was associated with tumor cell differentiation, clinical stage and lymph node metastasis (P<0. 05). MCM7 expression levels was associated with tumor cell differentiation and clinical stage (P<0. 05). HPV 16 E7 expression had no correlation with patient age, tumor size, tumor differentiation, clinical stage and lymph node metastasis (P>0. 05). Conclusion Aurora-A, MCM7 and HPV 16 E7 expression are gradually increased with disease progres-sion, and closely related to the occurrence and development of cervical cancer, they are expected to be early diagnosis, early treatment of biological indicators of cervical cancer.