CAJ | 학술논문

为了解黔产石韦不同极性部位萃取物对小鼠外周血白细胞数的影响，采用溶剂法对有柄石韦的不同极性部位进行萃取，用常规白细胞计数法评价不同极性部位萃取物对小鼠外周血白细胞数的影响，并用 HPLC 法分析其氨基酸成分。结果表明：黔产石韦的极性较大部位萃取物对小鼠外周血白细胞数有明显升高作用，极性较小部位无明显作用。水提组分可显著提高小鼠外周血白细胞数。黔产石韦中至少含有谷氨酸、甘氨酸、丙氨酸和亮氨酸4种氨基酸。其中，谷氨酸含量为1．189 mg／g，甘氨酸为0．70 mg／g，丙氨酸和亮氨酸含量，分别为0．36 mg／g 和0．343 mg／g。结论：谷氨酸可能是黔产石韦水提组分中增加小鼠外周血白细胞数的关键成分，甘氨酸具有协同作用。
위료해검산석위불동겁성부위췌취물대소서외주혈백세포수적영향，채용용제법대유병석위적불동겁성부위진행췌취，용상규백세포계수법평개불동겁성부위췌취물대소서외주혈백세포수적영향，병용 HPLC 법분석기안기산성분。결과표명：검산석위적겁성교대부위췌취물대소서외주혈백세포수유명현승고작용，겁성교소부위무명현작용。수제조분가현저제고소서외주혈백세포수。검산석위중지소함유곡안산、감안산、병안산화량안산4충안기산。기중，곡안산함량위1．189 mg／g，감안산위0．70 mg／g，병안산화량안산함량，분별위0．36 mg／g 화0．343 mg／g。결론：곡안산가능시검산석위수제조분중증가소서외주혈백세포수적관건성분，감안산구유협동작용。
The increasing white cell effect of extractions with different polarity from P .petiolosa produced in Guizhou were evaluated by solvent method and normal white cell count method,and amino acid constituents were analyzed through HPLC method.Results:The extractions with higher polarity increased the peripheral white blood cells of mice significantly,and the extractions with lower polarity can not.Especially,The effect of the water decocted liquid of P .petiolosa produced in Guizhou was the greatest on the mice peripheral white blood cells.It was found that that kinds of P .petiolosa contained four kinds of amino acids at least. Glu content was the highest (1.189 mg/g), followed by Gly (0.70 mg/g),Ala and Leu were the lowest,respectively 0.36 mg/g and 0.343 mg/g.Conclusion:Glu was presumed as the key components for increasing the mice peripheral white blood cells in the water decocted liquid of P .petiolosa produced in Guizhou,Gly was of synergistic effect.