CAJ | 학술논문

为我国南方荷斯坦奶牛抗热应激品种的选育提供理论依据，使用 PCR-SSCP 方法，对327头荷斯坦奶牛 HSP 70-1基因编码区进行单核苷酸多态性检测，并分析其与热应激的关联性。结果表明：HSP 70-1基因在1112位点发生 C→A 突变，有 CC、CA 和 AA 基因型，其中 CA 型个体血清三碘甲腺原氨酸（T3）显著低于其他基因型个体（P ＜0．05），甲状腺素（T4）显著低于 AA 型个体（P ＜0．05），直肠温度显著低于 CC 型个体（P ＜0．05）；在3390位点发生 T→C 突变，有 TT、TC 和 CC 基因型。1112位点的皮质醇（Cor）及3390位点各指标基因型间差异均不显著（P ＞0．05）。CA 型个体对抗热应激的能力更强，1112多态位点可以作为提高奶牛抗热应激能力的一个潜在遗传标记。
위아국남방하사탄내우항열응격품충적선육제공이론의거，사용 PCR-SSCP 방법，대327두하사탄내우 HSP 70-1기인편마구진행단핵감산다태성검측，병분석기여열응격적관련성。결과표명：HSP 70-1기인재1112위점발생 C→A 돌변，유 CC、CA 화 AA 기인형，기중 CA 형개체혈청삼전갑선원안산（T3）현저저우기타기인형개체（P ＜0．05），갑상선소（T4）현저저우 AA 형개체（P ＜0．05），직장온도현저저우 CC 형개체（P ＜0．05）；재3390위점발생 T→C 돌변，유 TT、TC 화 CC 기인형。1112위점적피질순（Cor）급3390위점각지표기인형간차이균불현저（P ＞0．05）。CA 형개체대항열응격적능력경강，1112다태위점가이작위제고내우항열응격능력적일개잠재유전표기。
The relationship between heat stress and polymorphism of HSP70-1 gene was studied by analyzing single nucleotide polymorphism in HSP70-1 coding sequence of Holstein cow by pcr-sscp to provide the theoretical basis for breeding of Holstein cow with resistance to heat stress in Southern China. Results:There is C→A mutation (CC,CA and AA genotypes)on 1112 locus of HSP70-1 gene.T3 ,T4 and rectal temperature of the individuals with CA genotype are lower than other individuals with other genotypes,individuals with AA genotype and individuals with CC genotype significantly.There is T→C mutation (TT,TC and CC genotypes)on 3390 locus of HSP70-1 gene.There is no significant difference in Cor on 1112 locus and different indexes on 3390 locus between different genotypes. The 1112 polymorphism locus can be used as a potential genetic marker to improve resistance to heat stress of Holstein cow because individual with CA genotype has of strong resistance to heat stress.