贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2015年
5期
149-152
,共4页
生防放线菌%酶活性%糖代谢%商洛%陕西
生防放線菌%酶活性%糖代謝%商洛%陝西
생방방선균%매활성%당대사%상락%협서
biocontrol actinomycetes%enzymatic activity%glycometabolism%Shangluo%Shaanxi
为获得更多特异性各异的生防放线菌，采用皿内琼脂块法和试管斜面划线法，对从陕西省商洛市土壤分离的11株生防放线菌菌株（MX1，MX2，MX3，MX4，MX5，MX6，MX7，MX8，MX9，SY1，SY2）和供试细黄链霉菌进行生理生化试验。结果表明：有8株菌株产脂肪酶，SY1、MX1和细黄链霉菌的浑浊圈很明显，直径大于15 mm 的菌株共计4株；有10株菌株产生卵磷脂酶，直径大于15 mm 的菌株共计5株；有11株菌株对淀粉的水力较强，直径大于15 mm 的共计6株；仅4株菌株可水解酪蛋白。12株供试菌株革兰氏染色均呈阳性；V-P 试验，MX1、MX2和 SY1呈阳性，MX6呈弱阳性；甲基红试验，MX2、MX3和细黄链霉菌呈阳性，MX9呈弱阳性；共有9株菌株可使明胶液化，占供试菌株的75％；仅 SY2产纤维素酶，有3株菌株产过氧化氢酶；有2株菌株能使牛奶培养基凝固，4株菌株能使其胨化。
為穫得更多特異性各異的生防放線菌，採用皿內瓊脂塊法和試管斜麵劃線法，對從陝西省商洛市土壤分離的11株生防放線菌菌株（MX1，MX2，MX3，MX4，MX5，MX6，MX7，MX8，MX9，SY1，SY2）和供試細黃鏈黴菌進行生理生化試驗。結果錶明：有8株菌株產脂肪酶，SY1、MX1和細黃鏈黴菌的渾濁圈很明顯，直徑大于15 mm 的菌株共計4株；有10株菌株產生卵燐脂酶，直徑大于15 mm 的菌株共計5株；有11株菌株對澱粉的水力較彊，直徑大于15 mm 的共計6株；僅4株菌株可水解酪蛋白。12株供試菌株革蘭氏染色均呈暘性；V-P 試驗，MX1、MX2和 SY1呈暘性，MX6呈弱暘性；甲基紅試驗，MX2、MX3和細黃鏈黴菌呈暘性，MX9呈弱暘性；共有9株菌株可使明膠液化，佔供試菌株的75％；僅 SY2產纖維素酶，有3株菌株產過氧化氫酶；有2株菌株能使牛奶培養基凝固，4株菌株能使其胨化。
위획득경다특이성각이적생방방선균，채용명내경지괴법화시관사면화선법，대종합서성상락시토양분리적11주생방방선균균주（MX1，MX2，MX3，MX4，MX5，MX6，MX7，MX8，MX9，SY1，SY2）화공시세황련매균진행생리생화시험。결과표명：유8주균주산지방매，SY1、MX1화세황련매균적혼탁권흔명현，직경대우15 mm 적균주공계4주；유10주균주산생란린지매，직경대우15 mm 적균주공계5주；유11주균주대정분적수력교강，직경대우15 mm 적공계6주；부4주균주가수해락단백。12주공시균주혁란씨염색균정양성；V-P 시험，MX1、MX2화 SY1정양성，MX6정약양성；갑기홍시험，MX2、MX3화세황련매균정양성，MX9정약양성；공유9주균주가사명효액화，점공시균주적75％；부 SY2산섬유소매，유3주균주산과양화경매；유2주균주능사우내배양기응고，4주균주능사기동화。
The biophysical and biochemical test of 11 actinomycetes strains(MX1,MX2,MX3,MX4, MX5,MX6,MX7,MX8,MX9,SY1 and SY2)isolated from soil of Shangluo City in Shaanxi and tested Streptomyces microflavus was conducted by the agar block and test-tube slant streak plate methods to obtain more biocontrol actinomycetes with different specificities.Results:The diameter of hydrolyzation circle of 4 strains from 8 strains with producing lipase is more than 15 mm, and SY1, MX1 and Streptomyces microflavus strains have obvious hydrolyzation circles.The diameter of hydrolyzation circle of 5 strains from 10 strains with producing lecithinase is more than 15 mm.The diameter of hydrolyzation circle of 6 strains from 11 strains with strong starch hydrolysis capacity is more than 15 mm.4 strains can hydrolyze casein.12 tested strains present positive in gram stain test.MX1,MX2 and SY1 strains present positive and MX6 strain presents weakly positive in V-P test.MX2,MX3 and Streptomyces microflavus strains present positive and MX9 strain presents weakly positive in methyl red test.9 strains are of gelatin liquefaction capacity.Only SY2 strain can produce cellulose.3 strains can produce catalase.2 strains have the capacity of solidifying milk medium and 4 strains have the capacity of peptonizing milk.
