CAJ | 학술논문

为白刺花新品种的培育提供理论依据，通过设置不同辐照剂量处理，以经辐照处理的白刺花种子培养的幼苗为研究对象，测定60 Co-γ辐射白刺花幼苗的相关生理指标，采用主成分分析和隶属函数分析法综合评价60 Co-γ射线对白刺花幼苗的诱变效果。结果表明：随着辐射剂量的增加，白刺花的叶绿素、MDA、可溶性糖和可溶性蛋白含量以及 POD 活性呈先升高后降低变化趋势，SOD 活性和脯氨酸含量则随着辐射剂量增加而逐渐升高。不同辐照剂量均可促进白刺花幼苗的光合作用，60 kR 时，MDA 含量达59.42μmol/ g，显著高于其他处理；100 kR 和120 kR 时，POD/ SOD 活性分别达峰值，为596.92 U/（g·min）和178.04 U/ g FW；100 kR 时，白刺花幼苗可溶性糖和可溶性蛋白含量最高；120 kR 时，脯氨酸含量最高。经主成分分析和隶属函数法对辐射白刺花生理指标进行综合评价，不同辐射剂量对白刺花生理指标的综合排序依次为120 kR﹥100 kR﹥80 kR﹥140 kR﹥60 kR﹥40 kR﹥20 kR﹥0 kR，以80 kR、100 kR和120 kR为较佳辐射剂量。
위백자화신품충적배육제공이론의거，통과설치불동복조제량처리，이경복조처리적백자화충자배양적유묘위연구대상，측정60 Co-γ복사백자화유묘적상관생리지표，채용주성분분석화대속함수분석법종합평개60 Co-γ사선대백자화유묘적유변효과。결과표명：수착복사제량적증가，백자화적협록소、MDA、가용성당화가용성단백함량이급 POD 활성정선승고후강저변화추세，SOD 활성화포안산함량칙수착복사제량증가이축점승고。불동복조제량균가촉진백자화유묘적광합작용，60 kR 시，MDA 함량체59.42μmol/ g，현저고우기타처리；100 kR 화120 kR 시，POD/ SOD 활성분별체봉치，위596.92 U/（g·min）화178.04 U/ g FW；100 kR 시，백자화유묘가용성당화가용성단백함량최고；120 kR 시，포안산함량최고。경주성분분석화대속함수법대복사백자화생리지표진행종합평개，불동복사제량대백자화생리지표적종합배서의차위120 kR﹥100 kR﹥80 kR﹥140 kR﹥60 kR﹥40 kR﹥20 kR﹥0 kR，이80 kR、100 kR화120 kR위교가복사제량。
The relative physiological indexes of seedlings from S. viciifolia seeds irradiated by different dosage of 60 Co-γ treatment and the mutation effect of S. viciifolia seedlings was comprehensively evaluated by principal component analysis(PCA)and membership function analysis to provide the theoretical basis for breeding of S.viciifolia variety. The results show that chlorophyll,MDA,soluble sugar and soluble protein content and POD activity of S. viciifolia seedlings both represent a first rising and then decline trend with increase of irradiation dosage but the SOD activity and proline content gradually increase with increase of irradiation dosage. The different irradiation treatment can improve photosynthesis of S. viciifolia seedlings. MDA content of seedlings of S. viciifolia seeds treated with 60 kR is up to 59. 42 μmol/ g,significantly higher than other treatments. The POD and SOD activity of seedlings of S. viciifolia seeds treated with 100 kR and 120 kR reaches 596. 92 U/(g·min)and 178. 04 U/ g FW respectively. The soluble sugar and soluble protein content of seedlings of S. viciifolia seeds treated with 100 kR is the maximum. The proline content of seedlings of S. viciifolia seeds treated with 120 kR is seedlings’ physiological indexes affected by irradiation dosage is 120 kR ﹥ 100 kR ﹥ 80 kR ﹥ 140 kR ﹥ 60 kR ﹥ 40 kR ﹥ 20 kR﹥0 kR and the optimum irradiation dosage for S. viciifolia seeds is 80 kR,100 kR and 120 kR.