CAJ | 학술논문

为了对粗毛淫羊藿野生资源的保护及淫羊藿黄酮离体生产体系提供技术参考，以粗毛淫羊藿幼叶为外植体，研究粗毛淫羊藿愈伤组织诱导的灭菌剂、消毒条件、培养基、激素配比和光照条件，并测定愈伤组织中总黄酮的含量。结果表明：利用粗毛淫羊藿幼叶进行愈伤组织培养，用2％的 NaClO 消毒14 min，接种于培养基 MS＋2，4-D 2．0 mg／L＋6-BA 0．5 mg／L＋IBA 0．5 mg／L＋NAA 1 mg／L，在24℃光照强度为1000 lx 条件下进行诱导效果最好，继代和保存应在适宜的黑暗条件下进行；诱导出的胚性愈伤组织总黄酮含量较高，达5．38％，与野生粗毛淫羊藿总黄酮含量想当，高于药典规定的标准。
위료대조모음양곽야생자원적보호급음양곽황동리체생산체계제공기술삼고，이조모음양곽유협위외식체，연구조모음양곽유상조직유도적멸균제、소독조건、배양기、격소배비화광조조건，병측정유상조직중총황동적함량。결과표명：이용조모음양곽유협진행유상조직배양，용2％적 NaClO 소독14 min，접충우배양기 MS＋2，4-D 2．0 mg／L＋6-BA 0．5 mg／L＋IBA 0．5 mg／L＋NAA 1 mg／L，재24℃광조강도위1000 lx 조건하진행유도효과최호，계대화보존응재괄의적흑암조건하진행；유도출적배성유상조직총황동함량교고，체5．38％，여야생조모음양곽총황동함량상당，고우약전규정적표준。
To provide technical references for protection of wild E.acuminatum resources and in vitro production system of flavonoids,the young leaves of E.acuminatum were used as explants for callus induction and its factors including different sterilization agent, medium, hormone combinations and illumination conditions which affect the callus induction of E.acuminatum were studied.Besides,the content of flavonoids in the calli was studied by means of the ultraviolet spectrophotometric analysis.The results indicate that the callus culture of E.acuminatum leaves can be induced under the following conditions:disinfection of 2% NaClO for 14 minutes,the medium MS + 2,4-D 2.0 mg/L+ 6-BA 0.5 mg/L+ IBA 0.5 mg/L + NAA 1 mg/L,at 24℃ 1 000 lx illumination conditions.The subculture and preservation is much appropriate under dark conditions.Total flavonoid content of induced embryonic calli is up to 5.38%,higher than that of pharmacopoeia standard.