CAJ | 학술논문

为快速、简便地定性定量荷叶中的黄酮类物质，建立高效液相色谱法测定荷叶中金丝桃苷、槲皮素、山奈酚和异鼠李素4种黄酮含量的方法。结果表明：在其分析测定条件色谱柱为 Gemini C18，柱温为40℃，流动相为甲醇－0．1％磷酸（55∶45，体积比），流速为1 mL／min，检测波长360 nm 的条件下，金丝桃苷、槲皮素、山奈酚和异鼠李素含量分别在1．5～48．0μg／mL，1．0～32．0μg／mL，1．0～32．0μg／mL，0．7～24．0μg／mL线性关系良好，方法的回收率为90％～103％，精密度为3．5％～4．2％，荷叶中4种黄酮含量依次为1．94％、0．092％、0．025％和0．016％。该方法可用于荷叶样品4种黄酮类物质的含量测定。
위쾌속、간편지정성정량하협중적황동류물질，건립고효액상색보법측정하협중금사도감、곡피소、산내분화이서리소4충황동함량적방법。결과표명：재기분석측정조건색보주위 Gemini C18，주온위40℃，류동상위갑순－0．1％린산（55∶45，체적비），류속위1 mL／min，검측파장360 nm 적조건하，금사도감、곡피소、산내분화이서리소함량분별재1．5～48．0μg／mL，1．0～32．0μg／mL，1．0～32．0μg／mL，0．7～24．0μg／mL선성관계량호，방법적회수솔위90％～103％，정밀도위3．5％～4．2％，하협중4충황동함량의차위1．94％、0．092％、0．025％화0．016％。해방법가용우하협양품4충황동류물질적함량측정。
To establish a quick and easy method of qualitative and quantitative analysis for determining the content of hyperoside,quercetin,kaempferol and isorhamnetin in lotus leaves by HPLC.Results:Under conditions of Gemini C18 for the chromatographic column and 40℃ for column temperature,with methanol-0.1% phosphoric aicd(55∶45,v∶v)of the mobile phase and 1 mL/min of flow velocity,360nm for the detection wavelength,the linear ranges of hyperoside,quercetin,kaempferol and isorhamnetin were 1.5~ 48.0 μg/mL,1.0 ~ 32.0 μg/mL,1.0 ~ 32.0 μg/mL,0.7 ~ 24.0 μg/mL,respectively,the recovery was 90%~103% and the precision was 3.5%~4.2%,the contents of four kinds of flavonoids in lotus leaves were respectively 1.94%,0.092%,0.025% and 0.021%.The proposed method was suitable for the determination of hyperoside,quercetin,kaempferol and isorhamnetin in lotus leaves.