CAJ | 학술논문

为弄清马铃薯生产中丛枝菌根真菌的作用，随机采取马铃薯块茎形成期的根系样品，以 DNA提取产物为基础，Nested-PCR 扩增目的片段，利用该产物构建 AMF 部分18S rRNA 基因克隆文库，运用ARDRA 筛选、DNA 测序和系统发育树等方法分析丛枝菌根真菌的结构组成。结果表明：文库 Coverage C值为89．7％，但 Rarefaction 曲线不够饱和；获得的8个 AMF 类型均与免培养的球囊霉属克隆序列相似度较高，其中 Seq2、Seq3、Seq5和 Seq8代表的摩西球囊霉（Glomus mosseae ）、幼套球囊霉（Glomus etunica-tum）、近明球囊霉（Glomus claroideum）和地表球囊霉（Glomus versifome ）分类地位较为清晰，其余序列可能代表的球囊霉属较新种类。
위롱청마령서생산중총지균근진균적작용，수궤채취마령서괴경형성기적근계양품，이 DNA제취산물위기출，Nested-PCR 확증목적편단，이용해산물구건 AMF 부분18S rRNA 기인극륭문고，운용ARDRA 사선、DNA 측서화계통발육수등방법분석총지균근진균적결구조성。결과표명：문고 Coverage C치위89．7％，단 Rarefaction 곡선불구포화；획득적8개 AMF 류형균여면배양적구낭매속극륭서렬상사도교고，기중 Seq2、Seq3、Seq5화 Seq8대표적마서구낭매（Glomus mosseae ）、유투구낭매（Glomus etunica-tum）、근명구낭매（Glomus claroideum）화지표구낭매（Glomus versifome ）분류지위교위청석，기여서렬가능대표적구낭매속교신충류。
In order to provide a reference for AMF applying in potato production,the roots of potato at the tuber formation stage were randomly sampled in this study.Based on the DNA extraction production, the purpose fragments were obtained by Nested-PCR.These DNA fragments were used to construct AMF partial 18S rRNA gene clone library.AMF community composition of potato roots were studied combining with ARDRA screening,DNA sequencing and phylogenetic tree analysis.These results show that the Coverage C value of library is 89.7%,but the rarefaction curve is not saturated enough.Eight AMF types show high similarity with uncultured Glomus sequences.The taxonomic status of Seq2,Seq3,Seq5 and Seq8 are clear.These four sequences represent Glomus mosseae ,Glomus etunicatum ,Glomus claroideum and Glomus versifome respectively.The remaining sequences may represent novel Glomus species.