贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2015年
5期
14-18
,共5页
朱丹丹%王培丹%林兴生%林辉%苏德伟%林占熺
硃丹丹%王培丹%林興生%林輝%囌德偉%林佔熺
주단단%왕배단%림흥생%림휘%소덕위%림점희
象草%巨菌草%染色体%核型
象草%巨菌草%染色體%覈型
상초%거균초%염색체%핵형
Pennisetum purpureum%Pennisetum giganteum%chromosome%karyotype
为巨菌草的种质鉴定、遗传育种和亲缘关系提供一定的细胞学基础,采用传统压片法,对象草(Pennisetum purpureum schumach)和巨菌草〔Pennisetum giganteum z.x.lin(暂定名)〕的染色体数目及核型进行分析。结果表明:象草和巨菌草的染色体形态较为一致,大部分由中部(m)和近中部(sm)着丝粒的染色体组成,象草和巨菌草的核型公式分别为2 n=4 x=28=20 m+8 sm 和2 n=4 x=28=16 m+8 sm+4 st;染色体相对长度组成分别为2 n=4 x=28=6 L+6 M2+10 M1+6 S 和2 n=4 x=28=4 L+8 M2+10 M1+6 S,染色体基数和总数目一致,核型分类均为2 A 型,不对称系数为58%~60%,均属于较原始的类型。结论:象草和巨菌草的的染色体无明显差异性,从细胞染色体水平上认为两者亲缘关系较近。
為巨菌草的種質鑒定、遺傳育種和親緣關繫提供一定的細胞學基礎,採用傳統壓片法,對象草(Pennisetum purpureum schumach)和巨菌草〔Pennisetum giganteum z.x.lin(暫定名)〕的染色體數目及覈型進行分析。結果錶明:象草和巨菌草的染色體形態較為一緻,大部分由中部(m)和近中部(sm)著絲粒的染色體組成,象草和巨菌草的覈型公式分彆為2 n=4 x=28=20 m+8 sm 和2 n=4 x=28=16 m+8 sm+4 st;染色體相對長度組成分彆為2 n=4 x=28=6 L+6 M2+10 M1+6 S 和2 n=4 x=28=4 L+8 M2+10 M1+6 S,染色體基數和總數目一緻,覈型分類均為2 A 型,不對稱繫數為58%~60%,均屬于較原始的類型。結論:象草和巨菌草的的染色體無明顯差異性,從細胞染色體水平上認為兩者親緣關繫較近。
위거균초적충질감정、유전육충화친연관계제공일정적세포학기출,채용전통압편법,대상초(Pennisetum purpureum schumach)화거균초〔Pennisetum giganteum z.x.lin(잠정명)〕적염색체수목급핵형진행분석。결과표명:상초화거균초적염색체형태교위일치,대부분유중부(m)화근중부(sm)착사립적염색체조성,상초화거균초적핵형공식분별위2 n=4 x=28=20 m+8 sm 화2 n=4 x=28=16 m+8 sm+4 st;염색체상대장도조성분별위2 n=4 x=28=6 L+6 M2+10 M1+6 S 화2 n=4 x=28=4 L+8 M2+10 M1+6 S,염색체기수화총수목일치,핵형분류균위2 A 형,불대칭계수위58%~60%,균속우교원시적류형。결론:상초화거균초적적염색체무명현차이성,종세포염색체수평상인위량자친연관계교근。
To provide certain cellular basis for germplasm, breeding and genetic kinship of P .giganteum,The karyotype analysis and chromosome number of P .purpureum and P .giganteum were conducted by traditional squash method.The results showed that the karyotype morphology of materials were similar,consisted of metacentric chromosomes and sub-metacentric chromosomes mostly.In this study, the karyotype formulas of P . purpureum and P . giganteum were respectively 2 n=4 x=28=20 m+8 sm,2 n=4 x=28= 16 m+8 sm+4 st.Constitutions of relative length of two plants were 2 n=4 x=28=6 L+6 M2 + 10 M1 +6 S,2 n=4 L+8 M2 + 10 M1 + 6S.The karyotype of P .purpureum was the same as that of P .giganteum in chromosome basic number and total number.And two varieties were classed as 2A.The asymmetry coefficient were in the range of 58% to 60%,which demonstrated basic symmetry karyotypes. Conclusion: Both of the two plants had no significant differences in chromosomes between them and the relationship between them was close from cellular level.