中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2015年
3期
279-284
,共6页
硅油类/副作用%视网膜/超微结构%动物实验
硅油類/副作用%視網膜/超微結構%動物實驗
규유류/부작용%시망막/초미결구%동물실험
Silicone oils/adverse effects%Retina/ultrastructure%Retina/physiopathology%Animal experimentation
目的 对比分析硅油与重硅油眼内填充对兔眼视功能、视网膜超微结构及组织形态的影响.方法 健康标准家兔28只随机分为A、B、C组,分别为12、12、4只.所有兔常规行玻璃体切割手术,A组填充硅油,B组填充重硅油,C组填充平衡盐溶液.手术后4、8、12、24周,采用视网膜电图(ERG)和光相干断层扫描(OCT)检查,测量其b波振幅和后极部视网膜厚度;取各组兔眼视网膜全层组织,采用透射电子显微镜和光学显微镜观察视网膜超微结构及组织形态变化.结果 ERG检查结果显示,与C组比较,B组兔眼b波振幅于手术后4周开始下降,手术后8周下降明显;A组兔眼b波振幅于手术后8周开始下降,手术后24周下降明显.手术后8、12、24周,B组兔眼b波振幅下降幅度均较A组更大,差异均有统计学意义(P<0.05).OCT检查发现,手术后24周,A、B组兔眼后极部视网膜厚度均较C组降低,差异有统计学意义(P<0.05);B组兔眼后极部视网膜厚度下降幅度较A组更大,差异也有统计学意义(P<0.05).透射电子显微镜和光学显微镜观察发现,A、B组兔眼视网膜超微结构与组织形态分别于手术后12、8周出现严重病理改变.B组兔眼视网膜超微结构中的视锥视杆细胞、盘膜、线粒体、细胞质、细胞核等细胞器造成的水肿及坏死程度均较同时期A组更重.B组兔眼视网膜组织形态损害及神经节细胞、内核层细胞的变性程度均较同时期A组更重.且随着硅油或重硅油填充时间的延长,视网膜超微结构、视网膜组织形态的损害及细胞变性程度更为严重.结论 重硅油眼内填充后对兔眼视功能、视网膜超微结构与组织形态的同期影响均大于硅油,且随着时间的延长两种填充物对其的影响越发显著.
目的 對比分析硅油與重硅油眼內填充對兔眼視功能、視網膜超微結構及組織形態的影響.方法 健康標準傢兔28隻隨機分為A、B、C組,分彆為12、12、4隻.所有兔常規行玻璃體切割手術,A組填充硅油,B組填充重硅油,C組填充平衡鹽溶液.手術後4、8、12、24週,採用視網膜電圖(ERG)和光相榦斷層掃描(OCT)檢查,測量其b波振幅和後極部視網膜厚度;取各組兔眼視網膜全層組織,採用透射電子顯微鏡和光學顯微鏡觀察視網膜超微結構及組織形態變化.結果 ERG檢查結果顯示,與C組比較,B組兔眼b波振幅于手術後4週開始下降,手術後8週下降明顯;A組兔眼b波振幅于手術後8週開始下降,手術後24週下降明顯.手術後8、12、24週,B組兔眼b波振幅下降幅度均較A組更大,差異均有統計學意義(P<0.05).OCT檢查髮現,手術後24週,A、B組兔眼後極部視網膜厚度均較C組降低,差異有統計學意義(P<0.05);B組兔眼後極部視網膜厚度下降幅度較A組更大,差異也有統計學意義(P<0.05).透射電子顯微鏡和光學顯微鏡觀察髮現,A、B組兔眼視網膜超微結構與組織形態分彆于手術後12、8週齣現嚴重病理改變.B組兔眼視網膜超微結構中的視錐視桿細胞、盤膜、線粒體、細胞質、細胞覈等細胞器造成的水腫及壞死程度均較同時期A組更重.B組兔眼視網膜組織形態損害及神經節細胞、內覈層細胞的變性程度均較同時期A組更重.且隨著硅油或重硅油填充時間的延長,視網膜超微結構、視網膜組織形態的損害及細胞變性程度更為嚴重.結論 重硅油眼內填充後對兔眼視功能、視網膜超微結構與組織形態的同期影響均大于硅油,且隨著時間的延長兩種填充物對其的影響越髮顯著.
목적 대비분석규유여중규유안내전충대토안시공능、시망막초미결구급조직형태적영향.방법 건강표준가토28지수궤분위A、B、C조,분별위12、12、4지.소유토상규행파리체절할수술,A조전충규유,B조전충중규유,C조전충평형염용액.수술후4、8、12、24주,채용시망막전도(ERG)화광상간단층소묘(OCT)검사,측량기b파진폭화후겁부시망막후도;취각조토안시망막전층조직,채용투사전자현미경화광학현미경관찰시망막초미결구급조직형태변화.결과 ERG검사결과현시,여C조비교,B조토안b파진폭우수술후4주개시하강,수술후8주하강명현;A조토안b파진폭우수술후8주개시하강,수술후24주하강명현.수술후8、12、24주,B조토안b파진폭하강폭도균교A조경대,차이균유통계학의의(P<0.05).OCT검사발현,수술후24주,A、B조토안후겁부시망막후도균교C조강저,차이유통계학의의(P<0.05);B조토안후겁부시망막후도하강폭도교A조경대,차이야유통계학의의(P<0.05).투사전자현미경화광학현미경관찰발현,A、B조토안시망막초미결구여조직형태분별우수술후12、8주출현엄중병리개변.B조토안시망막초미결구중적시추시간세포、반막、선립체、세포질、세포핵등세포기조성적수종급배사정도균교동시기A조경중.B조토안시망막조직형태손해급신경절세포、내핵층세포적변성정도균교동시기A조경중.차수착규유혹중규유전충시간적연장,시망막초미결구、시망막조직형태적손해급세포변성정도경위엄중.결론 중규유안내전충후대토안시공능、시망막초미결구여조직형태적동기영향균대우규유,차수착시간적연장량충전충물대기적영향월발현저.
Objective To investigate the medium and long-term influence of silicon oil versus heavy silicone oil on rabbit retinas.Methods 28 health standard rabbits were randomly divided into A,B and C groups,with 12,12 and 4 rabbits respectively.All rabbits received routine vitrectomy and tamponade with silicone oil (group A),or heavy silicone oil (group B) or balanced salt solution (group C).After 4,8,12 and 24 weeks,the retinal b-wave amplitude was measured by ERG,posterior retinal thickness was measured by optical coherence tomography (OCT).Retinal ultrastructure and tissue morphology were observed by transmission electron microscopy and optical microscopy.Results Compare to group C,the b-wave amplitude decreased at 4 weeks after surgery,and decreased at 8 weeks after surgery for group B,and decreased at 8 weeks after surgery,and decreased at 24 weeks after surgery for group A.The decreases were greater in group B than group A at 8,12,24 weeks after surgery,the difference was statistically significant (P<0.05).The posterior retinal thickness of group A and B was thinner than group C at 24 weeks after surgery (P<0.05).The decreases were greater in group B than group A,the difference was statistically significant (P<0.05).Transmission electron microscopy and optical microscopy revealed severe pathological changes of retinal ultrastructure and morphology in group A and B rabbit eyes,at 12 weeks and 8 weeks after surgery respectively.The changes were more severe in group B and group A,including edema and necrosis in cone/rod cells,in disk membranes,mitochondria,cytoplasm,nucleus and other organelles.The morphological changes were also more severe in group B and group A,including degenerations of ganglion cell layer,inner nuclear layer changes.Those changes became more severe when the tamponade time extended.Conclusion The heavy silicone influence on visual function,ultrastructures,histomorphology of rabbit retinas is much worse than the silicon oil,and the effect is more significant with its time prolong.