CAJ | 학술논문

目的:本研究旨在探讨Toll样受体2(Toll-like receptors 2,TLR2)和TLR4活化对人骨髓源性间充质干细胞(BM-MSC)诱导人脐血CD34+造血干/祖细胞迁移功能的影响及其机制.方法:流式细胞术检测健康供体BM-MSC表面TLR2和TLR4的表达情况;TLR2激动剂(PAM3 CSK4)和TLR4激动剂(LPS)活化人BM-MSC,收集培养上清,趋化实验测定BM-MSC培养上清对人脐血CD34+细胞的趋化作用;了解TLR2或(和)TLR4的活化对BM-MSC诱导CD34+细胞迁移活性的影响.ELISA法定量检测培养上清中趋化因子SDF-1的分泌水平.结果:人BM-MSC表面表达TLR2水平分别为(31.5±4.6)％和TLR4(85.6±6.7)％.与未加培养上清的空白组相比,对照组、LPS和/或PAM3 CSK4活化的MSC的培养上清对CD34+均有明显趋化作用(P＜0.01).进一步研究显示,与未加激动剂的对照组(MSC上清组)相比,LPS组、PAM3CSK4组、LPS和PAM3CSK4联合刺激组的MSC培养上清诱导CD34+细胞的迁移均有显著促进作用(P＜0.05),但LPS组、PAM3CSK4组、LPS和PAM3CSK4联合刺激组的培养上清中,SDF-1浓度与对照组相比未见明显变化(P＞0.05).结论:TLR2和/或TLR4的活化可显著增强骨髓BM-MSC诱导人脐血CD34+细胞的迁移作用,但与趋化因子SDF-1无明显关系.
목적:본연구지재탐토Toll양수체2(Toll-like receptors 2,TLR2)화TLR4활화대인골수원성간충질간세포(BM-MSC)유도인제혈CD34+조혈간/조세포천이공능적영향급기궤제.방법:류식세포술검측건강공체BM-MSC표면TLR2화TLR4적표체정황;TLR2격동제(PAM3 CSK4)화TLR4격동제(LPS)활화인BM-MSC,수집배양상청,추화실험측정BM-MSC배양상청대인제혈CD34+세포적추화작용;료해TLR2혹(화)TLR4적활화대BM-MSC유도CD34+세포천이활성적영향.ELISA법정량검측배양상청중추화인자SDF-1적분비수평.결과:인BM-MSC표면표체TLR2수평분별위(31.5±4.6)％화TLR4(85.6±6.7)％.여미가배양상청적공백조상비,대조조、LPS화/혹PAM3 CSK4활화적MSC적배양상청대CD34+균유명현추화작용(P＜0.01).진일보연구현시,여미가격동제적대조조(MSC상청조)상비,LPS조、PAM3CSK4조、LPS화PAM3CSK4연합자격조적MSC배양상청유도CD34+세포적천이균유현저촉진작용(P＜0.05),단LPS조、PAM3CSK4조、LPS화PAM3CSK4연합자격조적배양상청중,SDF-1농도여대조조상비미견명현변화(P＞0.05).결론:TLR2화/혹TLR4적활화가현저증강골수BM-MSC유도인제혈CD34+세포적천이작용,단여추화인자SDF-1무명현관계.