中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2015年
4期
316-319
,共4页
李媛媛%崔凌凌%李鑫德%王敬丽%李长贵%苗志敏%刘世国
李媛媛%崔凌凌%李鑫德%王敬麗%李長貴%苗誌敏%劉世國
리원원%최릉릉%리흠덕%왕경려%리장귀%묘지민%류세국
维生素D受体%多态性,单核苷酸%痛风
維生素D受體%多態性,單覈苷痠%痛風
유생소D수체%다태성,단핵감산%통풍
Vitmin D receptor%Polymorphism,single Nucleotide%Gout
目的 探讨中国汉族沿海地区男性群体的维生素D受体(vitmin D receptor,VDR) Fok Ⅰrs2228570(G/A)位点基因多态性与痛风遗传易感性的关系.方法 采用Taqman(R)荧光定量PCR技术,对青岛地区504例男性痛风患者与523名健康对照者rs2228570位点进行基因分型.采用Hardy-Weinberg检验确认标本的群体代表性,数据分析采用x2检验和t检验.结果 痛风组中VDR基因rs2228570位点GG、AG和AA基因型所占比例分别为32.1％、50.0％和17.9％,健康对照组中分别为27.9％、50.5％和21.6％,2组比较差异无统计学意义(x2=3.366,P＞0.05);2组的等位基因频率G和A间差异也无统计学意义(分别为57.1％,42.9％;53.2％,46.8％;x2=3.300,P＞0.05).结论 中国沿海地区汉族男性群VDR Fok Ⅰ rs2228570位点基因多态性与痛风可能没有关联性.
目的 探討中國漢族沿海地區男性群體的維生素D受體(vitmin D receptor,VDR) Fok Ⅰrs2228570(G/A)位點基因多態性與痛風遺傳易感性的關繫.方法 採用Taqman(R)熒光定量PCR技術,對青島地區504例男性痛風患者與523名健康對照者rs2228570位點進行基因分型.採用Hardy-Weinberg檢驗確認標本的群體代錶性,數據分析採用x2檢驗和t檢驗.結果 痛風組中VDR基因rs2228570位點GG、AG和AA基因型所佔比例分彆為32.1％、50.0％和17.9％,健康對照組中分彆為27.9％、50.5％和21.6％,2組比較差異無統計學意義(x2=3.366,P＞0.05);2組的等位基因頻率G和A間差異也無統計學意義(分彆為57.1％,42.9％;53.2％,46.8％;x2=3.300,P＞0.05).結論 中國沿海地區漢族男性群VDR Fok Ⅰ rs2228570位點基因多態性與痛風可能沒有關聯性.
목적 탐토중국한족연해지구남성군체적유생소D수체(vitmin D receptor,VDR) Fok Ⅰrs2228570(G/A)위점기인다태성여통풍유전역감성적관계.방법 채용Taqman(R)형광정량PCR기술,대청도지구504례남성통풍환자여523명건강대조자rs2228570위점진행기인분형.채용Hardy-Weinberg검험학인표본적군체대표성,수거분석채용x2검험화t검험.결과 통풍조중VDR기인rs2228570위점GG、AG화AA기인형소점비례분별위32.1％、50.0％화17.9％,건강대조조중분별위27.9％、50.5％화21.6％,2조비교차이무통계학의의(x2=3.366,P＞0.05);2조적등위기인빈솔G화A간차이야무통계학의의(분별위57.1％,42.9％;53.2％,46.8％;x2=3.300,P＞0.05).결론 중국연해지구한족남성군VDR Fok Ⅰ rs2228570위점기인다태성여통풍가능몰유관련성.
Objective To explore gene polymorphism of G/A genotype of Fok Ⅰ rs2228570 (G/A) of vitamin D receptor (VDR) gene in Han male population of Chinese coastal area,and thus to investigate the relationship between the gene polymorphism of VDR and gout.Methods Altogether 504 gout patients and 523 healthy controls were enrolled.The possible association between the polymorphism of VDR rs2228570 and gout in Chinese coastal area was investigated and genotype frequencies and allelic frequencies were calculated by realtime PCR with Taqman(R)probe method.Hardy-Weinberg was used to verify the representativeness of the sample.Comparison between the groups were performed withx2 test and t-test.Results The frequencies of GG,AG,and AA genotypes were 32.1％,50.0％,and 17.9％,respectively among gout patients,while they were 27.9％,50.5％,and 21.6％ respectively among the controls.There was no statistically significant difference in VDR rs2228570 genotype frequencies between gout patients and controls(x2 =3.366,P＞0.05).The allele frequencies of G and A in gout cases were different from those in the controls(57.1％,42.9％;53.2％,46.8％;x2 =3.300,P＞0.05).Conclusions Results of the present study suggest that the G/A genotype of VDR Fok Ⅰ rs2228570 of the VDR gene is not associated with gout in male population of Chinese coastal area.
