CAJ | 학술논문

目的探讨骨桥蛋白(OPN)对干扰素-γ(IFN-γ)诱导甲状腺细胞分泌趋化因子(C-X-C结构域)配体9(CXCL9)的影响及其在Graves病发生发展中的意义.方法取10例Graves病患者及9名正常对照者手术切除甲状腺组织标本,分离培养Graves病患者及正常对照者的甲状腺原代细胞,通过实时荧光定量PCR技术,比较两者CXCL9及OPN的表达情况.体外培养人正常甲状腺细胞系Nthy-ori3-1和正常人甲状腺原代细胞,分别用不同浓度的OPN及IFN-γ进行刺激实验,利用实时荧光定量PCR技术,检测甲状腺细胞趋化因子CXCL9的mRNA表达情况.结果 Graves病患者与正常对照者甲状腺组织中均存在OPN及CXCL9的表达,与正常对照者比较,Graves病患者甲状腺组织高表达CXCL9及OPN(P＜0.05).IFN-γ可诱导正常甲状腺细胞系Nthy-ori3-1和人甲状腺原代细胞表达CXCL9(P＜0.01),单独的OPN刺激对CXCL9的表达无明显影响.OPN呈浓度依赖性抑制IFN-γ诱导人正常甲状腺细胞系Nthy-ori3-1分泌趋化因子CXCL9(P＜0.01),并在一定程度上抑制IFN-γ诱导人甲状腺原代细胞分泌趋化因子CXCL9,但差异无统计学意义(P＞0.05).结论 Graves病中,甲状腺局部OPN表达升高,可能通过与其他促炎因子相拮抗而起到保护作用,这可能是机体的一种代偿反应.
목적 탐토골교단백(OPN)대간우소-γ(IFN-γ)유도갑상선세포분비추화인자(C-X-C결구역)배체9(CXCL9)적영향급기재Graves병발생발전중적의의.방법 취10례Graves병환자급9명정상대조자수술절제갑상선조직표본,분리배양Graves병환자급정상대조자적갑상선원대세포,통과실시형광정량PCR기술,비교량자CXCL9급OPN적표체정황.체외배양인정상갑상선세포계Nthy-ori3-1화정상인갑상선원대세포,분별용불동농도적OPN급IFN-γ진행자격실험,이용실시형광정량PCR기술,검측갑상선세포추화인자CXCL9적mRNA표체정황.결과 Graves병환자여정상대조자갑상선조직중균존재OPN급CXCL9적표체,여정상대조자비교,Graves병환자갑상선조직고표체CXCL9급OPN(P＜0.05).IFN-γ가유도정상갑상선세포계Nthy-ori3-1화인갑상선원대세포표체CXCL9(P＜0.01),단독적OPN자격대CXCL9적표체무명현영향.OPN정농도의뢰성억제IFN-γ유도인정상갑상선세포계Nthy-ori3-1분비추화인자CXCL9(P＜0.01),병재일정정도상억제IFN-γ유도인갑상선원대세포분비추화인자CXCL9,단차이무통계학의의(P＞0.05).결론 Graves병중,갑상선국부OPN표체승고,가능통과여기타촉염인자상길항이기도보호작용,저가능시궤체적일충대상반응.
Objective To investigate the effect of osteopontin (OPN) on interferon-γ (IFN-γ)-induced expression of chemokine (C-X-C motif) ligand 9 (CXCL9) in thyrocytes and its significance in the development of Graves' disease.Methods Surgical tissues were obtained from ten patients with Graves' disease and nine normal controls,and the tissues were prepared for thyroid follicular cells.The expression of OPN and CXCL9 genes were detected by realtime quantitative PCR.The expression of CXCL9 genes in cultured Nthy-ori3-1 cell line and human thyrocytes stimulated by IFN-γand OPN were also determined by realtime quantitative PCR.Results Compared to normal controls,CXCL9 and OPN gene levels were significantly higher in Graves' disease thyroid tissues (P＜0.05).OPN inhibited IFN-γ-induced CXCL9 expression in Nthy-ori3-1 cell line in a concentration dependent manner (P＜0.01),while inhibited the expression in human thyrocytes to a certain extent but showed no difference (P ＞ 0.05).Conclusion The higher expression of OPN in thyroid tissue of Graves' disease may antagonize other proinflammtory cytokines,indicating that it may play a protective role and be a compensatory reaction to autoimmune response.