CAJ | 학술논문

目的探讨氢盐水对百草枯(PQ)中毒小鼠急性肺损伤以及对肺组织血红素氧化酶-1(HO-1)表达的影响及机制.方法 108只雄性清洁级ICR小鼠随机分为对照组(8只),PQ组(50只)和PQ+氢盐水组(50只),PQ组质量浓度为20％的PQ 50 mg/kg一次性灌胃.PQ+氢盐水组20％的PQ50 mg/kg一次性灌胃后予饱和氢气生理盐水5 ml/kg腹腔注射,2次/d.观察行为学变化,不同处理后6h、1、3、7、14d测定肺组织匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力,HE染色观察肺组织损伤情况,免疫印迹法和免疫组化法测定肺组织HO-1含量.结果 PQ中毒后小鼠出现活动减少,呼吸急促甚至死亡等表现,PQ+氢盐水组比PQ组有所减轻.PQ组6h及24 h肺组织匀浆MDA含量明显高于对照组,PQ组6、24h及3、7d时的SOD活力明显低于对照组,差异有统计学意义(P＜0.05).PQ+氢盐水组24 h MDA含量明显低于PQ组,24 h及3、7d时PQ+氢盐水组SOD活力明显高于PQ组,差异有统计学意义(P＜0.05).PQ+氢盐水组HO-1表达趋势及分布部位与PQ组相似,但各时间点HO-1表达HIS值均明显高于PQ组和对照组,差异均有统计学意义(P＜0.05).结论氢盐水可上调PQ中毒小鼠肺组织HO-1的表达,有减轻PQ中毒肺损伤的效应.
목적 탐토경염수대백초고(PQ)중독소서급성폐손상이급대폐조직혈홍소양화매-1(HO-1)표체적영향급궤제.방법 108지웅성청길급ICR소서수궤분위대조조(8지),PQ조(50지)화PQ+경염수조(50지),PQ조질량농도위20％적PQ 50 mg/kg일차성관위.PQ+경염수조20％적PQ50 mg/kg일차성관위후여포화경기생리염수5 ml/kg복강주사,2차/d.관찰행위학변화,불동처리후6h、1、3、7、14d측정폐조직균장병이철(MDA)함량、초양화물기화매(SOD)활력,HE염색관찰폐조직손상정황,면역인적법화면역조화법측정폐조직HO-1함량.결과 PQ중독후소서출현활동감소,호흡급촉심지사망등표현,PQ+경염수조비PQ조유소감경.PQ조6h급24 h폐조직균장MDA함량명현고우대조조,PQ조6、24h급3、7d시적SOD활력명현저우대조조,차이유통계학의의(P＜0.05).PQ+경염수조24 h MDA함량명현저우PQ조,24 h급3、7d시PQ+경염수조SOD활력명현고우PQ조,차이유통계학의의(P＜0.05).PQ+경염수조HO-1표체추세급분포부위여PQ조상사,단각시간점HO-1표체HIS치균명현고우PQ조화대조조,차이균유통계학의의(P＜0.05).결론 경염수가상조PQ중독소서폐조직HO-1적표체,유감경PQ중독폐손상적효응.
Objective To investigate the effect of Hydrogen-saline on Lung Injury and HO-1 Expression in The Lung Tissue of Acute Paraquat-intoxicated mice.Methods 108 male clean-grade mice were divided randomly into 3 groups:normal control group(n=8),PQ group(n=50) and PQ HN group(n=50),PQ group were lavaged with 20％ PQ(50 mg/kg).PQ+Hyclrogen saline group were intxaperitoneal injected with 5 ml/kg saturated hydragen saline after lavaged,2 times/d.We observe its performance after the poisoning,The lung tissue were taken to HE stained,MDA and SOD activity of lung tissue homogenate were detected,HO-1 activity were observed by immunohistochemistry and western method at 6 h,1 d,3 d,7 d and 14 d.Results mice came to shortness of breath,exhaustion and death after poisoned.PQ+hydrogen saline group was more alleviative than PQ group.The lung MDA of PQ group was markedly increased at 6 h and 24 h and SOD was decreased at 6 h,24 h,3 d,7 d,14 d than normal control group.In Comparison with PQ group,the lung MDA was decreased at 24 h and SOD was increased at 24 h and 3 d (P＜0.05).HO-1 expression trend and distribution in PQ+hydrogen saline group are similar with PQ group,but were significantly higher than that of PQ group and the control group each time point (P＜0.05).Conclusion Oxidative stress plays important roles in lung injury caused by paraquat.Hydrogen-saline may increase expression of HO-1 and alleviate oxidative stress damage in lung.