CAJ | 학술논문

目的研究肝细胞生长因子激活因子(HGFA)的抑制因子(HAI-1、HAI-2)在硬化和正常肝脏再生中的表达,探讨HAI-1、HAI-2的生物学效应及其与肝再生的关系.方法采用40％四氯化碳油溶液背部皮下注射法建立大鼠肝硬化模型.实验组实施70％肝脏切除术,建立部分肝切除术后再生模型,以健康大鼠作为对照.分别于术前及术后3、6、12、24、48 h处死大鼠并留取标本检测.使用实时PCR技术检测脾原性HAI-1 mRNA、HAI-2 mRNA表达情况.结果肝硬化大鼠各时间点血清HGFA浓度均显著低于对照组(P＜0.05),肝硬化大鼠HAI-1 mRNA表达量持续高于对照组(P＜0.05),而两组大鼠之间HAI-2 mRNA表达量差异无统计学意义(P＞0.05).结论肝硬化大鼠部分肝切除术后再生过程中HGFA的合成分泌较健康大鼠低,这可能导致了肝细胞生长因子(HGF)前体活化不足,以致再生速率缓慢.HAI-2并没有参与肝脏的损伤修复进程.
목적 연구간세포생장인자격활인자(HGFA)적억제인자(HAI-1、HAI-2)재경화화정상간장재생중적표체,탐토HAI-1、HAI-2적생물학효응급기여간재생적관계.방법 채용40％사록화탄유용액배부피하주사법건립대서간경화모형.실험조실시70％간장절제술,건립부분간절제술후재생모형,이건강대서작위대조.분별우술전급술후3、6、12、24、48 h처사대서병류취표본검측.사용실시PCR기술검측비원성HAI-1 mRNA、HAI-2 mRNA표체정황.결과 간경화대서각시간점혈청HGFA농도균현저저우대조조(P＜0.05),간경화대서HAI-1 mRNA표체량지속고우대조조(P＜0.05),이량조대서지간HAI-2 mRNA표체량차이무통계학의의(P＞0.05).결론 간경화대서부분간절제술후재생과정중HGFA적합성분비교건강대서저,저가능도치료간세포생장인자(HGF)전체활화불족,이치재생속솔완만.HAI-2병몰유삼여간장적손상수복진정.
Objective To investigate the differential expression of hepatocyte growth factor activator (HGFA) and its inhibitors (HAI-1,HAI-2) during cirrhotic and normal liver regeneration after partial hepatectomy,and to explore the causes of the delayed liver regeneration after partial hepatectomy in cirrhotic rat model.Methods We used 40％ CCl4 subcutaneous injection to establish the cirrhotic rat model,and then performed 70％ liver resection for the experimental group together with no operation for the healthy rats as control group.Rats in each group after 3 hours,6 hours,12 hours,24 hours and 48 hours were randomly sacrificed and specimens were collected.The serum HGFA was detected by enzyme-linked immunosorbent assay (ELISA),and we used RT-PCR to detect the mRNA expressions of HAI-1 and HAI-2 in splenic tissue.Results The serum HGFA level in cirrhotic rats at each time point was all significantly lower than that in the control group (P ＜0.05).The expression of HAI-1 mRNA in cirrhotic rats was sustained at a higher level than that in the control group (P ＜ 0.05),but there was no significant difference on the HAI-2 mRNA expression between the two groups (P ＞ 0.05).Conclusions The synthesis of HGFA during the liver regeneration after partial hepatectomy in cirrhosis rats is lower compared with healthy rats,which may lead to the insufficient activation of HGF precursor,eventually causing the slow liver regeneration.HAI-2 may not be involved in the healing process of liver.