CAJ | 학술논문

目的：研究抗血小板药替格瑞洛对大鼠颈总动脉损伤后内膜增生的影响及机制。方法把42只普通SD大鼠用随机法分为正常对照组（6只）、球囊损伤组（12只）、替格瑞洛正常剂量组（12只）、替格瑞洛高剂量组（12只）。术后7天、21天取颈总动脉行H-E染色，观察颈总动脉病理形态变化，并测量内膜的面积（ IA）和中膜的面积（ MA）。免疫组化技术测定PDGF、PCNA的表达。结果药物正常剂量和高剂量组与球囊损伤组比较，7天、21天内膜的面积、内膜/中膜的面积比明显减少（ P <0.05）。颈总动脉的 PDGF、PCNA 的表达明显降低（ P <0.05）。药物正常剂量组与药物高剂量组比较，7天时药物高剂量组的内膜面积、内膜/中膜的面积比明显减少（ P<0.05）。颈总动脉的PDGF、PCNA的表达明显降低（ P<0.05）。药物高剂量组与正常药物剂量组21天与7天的内膜面积差比较无明显差异。颈总动脉的PDGF、PCNA的表达无明显差异。结论替格瑞洛能够减轻动脉损伤后内膜增生的程度，其可能机制为抑制血小板的活化，阻断血小板释放PDGF刺激血管平滑肌增生。球囊损伤前期高剂量药物组较正常剂量组抑制内膜增生较为明显，后期高剂量组与正常剂量组无明显差异。
목적：연구항혈소판약체격서락대대서경총동맥손상후내막증생적영향급궤제。방법파42지보통SD대서용수궤법분위정상대조조（6지）、구낭손상조（12지）、체격서락정상제량조（12지）、체격서락고제량조（12지）。술후7천、21천취경총동맥행H-E염색，관찰경총동맥병리형태변화，병측량내막적면적（ IA）화중막적면적（ MA）。면역조화기술측정PDGF、PCNA적표체。결과약물정상제량화고제량조여구낭손상조비교，7천、21천내막적면적、내막/중막적면적비명현감소（ P <0.05）。경총동맥적 PDGF、PCNA 적표체명현강저（ P <0.05）。약물정상제량조여약물고제량조비교，7천시약물고제량조적내막면적、내막/중막적면적비명현감소（ P<0.05）。경총동맥적PDGF、PCNA적표체명현강저（ P<0.05）。약물고제량조여정상약물제량조21천여7천적내막면적차비교무명현차이。경총동맥적PDGF、PCNA적표체무명현차이。결론체격서락능구감경동맥손상후내막증생적정도，기가능궤제위억제혈소판적활화，조단혈소판석방PDGF자격혈관평활기증생。구낭손상전기고제량약물조교정상제량조억제내막증생교위명현，후기고제량조여정상제량조무명현차이。
Objective:To investigate the effect of anti-platelet drug ticagrelor on carotid artery intimal hyperplasia in rats after balloon injury and its mechanism. Methods:Forty-two ordinary SD rats were randomly divided into normal control group(n=6),balloon injury group(n=12),ticagrelor normal dose group(n=12),ticagrelor high-dose group(n=12). Carotid artery of four groups was taken out at 7 days and 21 days after balloon injury. The pathological changes of ca-rotid artery was observed by H-E staining and intimal area( IA)and the membrane area( MA)was measured. PDGF and PCNA expressions were determined by immunohistochemical method. Results:Compared with the balloon injury group,the intimal area and the intima / media area ratio was significantly reduced in the high dose group and normal dose group( P<0. 05). Carotid artery PDGF and PCNA expressions were significantly lower(P<0. 05). Compared with the normal dose groups,at 7 day points intimal area and the intima / media area ratio were significantly reduced in high dose group( P<0. 05). Carotid artery PDGF amd PCNA expressions were significantly lower,too(P<0. 05). High-dose group and normal drug dose group showed no significant difference in the intimal area between 21 days and 7 days. At the same time,there was no significant difference in the expression of the common carotid artery PDGF and PCNA. Conclusion:Ticagrelor can reduce the extent of intimal hyperplasia after arterial injury and its mechanism probably includes inhibition of platelet activa-tion,blocking platelet release PDGF stimulating vascular smooth muscle proliferation. Early balloon injury has more obvi-ous inhibition of intimal hyperplasia in the high dose drug group than in the normal dose group,and at the later stage high dose group has no difference from the normal dose group.