针灸推拿医学（英文版）
針灸推拿醫學（英文版）
침구추나의학（영문판）
JOURNAL OF ACUPUNCTURE AND TUINA SCIENCE
2015年
3期
146-149
,共4页
腕踝针%痛经%针刺镇痛%内啡呔%一氧化氮%前列腺素F类%P物质%大鼠
腕踝針%痛經%針刺鎮痛%內啡呔%一氧化氮%前列腺素F類%P物質%大鼠
완과침%통경%침자진통%내배태%일양화담%전렬선소F류%P물질%대서
Wrist-ankle Acupuncture%Dysmenorrhea%Acupuncture Analgesia%Endorphins%Nitric Oxide%Prostaglandins F%Substance P%Rats
目的：观察腕踝针对原发性痛经大鼠子宫组织匀浆中β-内啡肽(β-endorphin, EP)、一氧化氮(nitric oxide, NO)及血清中前列腺素F2a (Prostaglandin F2α, PGF2α)、P物质(Substance P, SP)的影响。方法：将45只未孕Wistar大鼠按随机数字表法分为空白对照组、模型组、腕踝针组，每组15只。除空白对照组外，其余两组在大鼠腹部皮下连续注射已烯雌酚建立痛经模型,腕踝针组造模成功后第1天起针刺下1区、下2区，每日1次，连续10天。空白对照组和模型组不予任何治疗。观察大鼠的扭体潜伏期与扭体次数，检测子宫组织匀浆中β-EP、NO及血清中PGF2α、SP含量。结果：模型组大鼠子宫组织匀浆中β-EP、NO水平最低，血清中的PGF2α水平最高，SP含量最低，与空白对照组比较,组间差异有统计学意义(P＜0.05)。腕踝针组PGF2α低于模型组,β-EP、NO、SP水平高于模型组,组间差异有统计学意义(P＜0.05)。结论：腕踝针可能通过抑制PGF2α,上调β-EP、NO及SP缓解子宫痉挛,增加血流量,促进子宫组织功能改善,达到止痛的效果。
目的：觀察腕踝針對原髮性痛經大鼠子宮組織勻漿中β-內啡肽(β-endorphin, EP)、一氧化氮(nitric oxide, NO)及血清中前列腺素F2a (Prostaglandin F2α, PGF2α)、P物質(Substance P, SP)的影響。方法：將45隻未孕Wistar大鼠按隨機數字錶法分為空白對照組、模型組、腕踝針組，每組15隻。除空白對照組外，其餘兩組在大鼠腹部皮下連續註射已烯雌酚建立痛經模型,腕踝針組造模成功後第1天起針刺下1區、下2區，每日1次，連續10天。空白對照組和模型組不予任何治療。觀察大鼠的扭體潛伏期與扭體次數，檢測子宮組織勻漿中β-EP、NO及血清中PGF2α、SP含量。結果：模型組大鼠子宮組織勻漿中β-EP、NO水平最低，血清中的PGF2α水平最高，SP含量最低，與空白對照組比較,組間差異有統計學意義(P＜0.05)。腕踝針組PGF2α低于模型組,β-EP、NO、SP水平高于模型組,組間差異有統計學意義(P＜0.05)。結論：腕踝針可能通過抑製PGF2α,上調β-EP、NO及SP緩解子宮痙攣,增加血流量,促進子宮組織功能改善,達到止痛的效果。
목적：관찰완과침대원발성통경대서자궁조직균장중β-내배태(β-endorphin, EP)、일양화담(nitric oxide, NO)급혈청중전렬선소F2a (Prostaglandin F2α, PGF2α)、P물질(Substance P, SP)적영향。방법：장45지미잉Wistar대서안수궤수자표법분위공백대조조、모형조、완과침조，매조15지。제공백대조조외，기여량조재대서복부피하련속주사이희자분건립통경모형,완과침조조모성공후제1천기침자하1구、하2구，매일1차，련속10천。공백대조조화모형조불여임하치료。관찰대서적뉴체잠복기여뉴체차수，검측자궁조직균장중β-EP、NO급혈청중PGF2α、SP함량。결과：모형조대서자궁조직균장중β-EP、NO수평최저，혈청중적PGF2α수평최고，SP함량최저，여공백대조조비교,조간차이유통계학의의(P＜0.05)。완과침조PGF2α저우모형조,β-EP、NO、SP수평고우모형조,조간차이유통계학의의(P＜0.05)。결론：완과침가능통과억제PGF2α,상조β-EP、NO급SP완해자궁경련,증가혈류량,촉진자궁조직공능개선,체도지통적효과。
Objective:To observe effects of wrist-ankle acupuncture (WAA) onβ-endorphin (EP), nitric oxide (NO) in uterus tissue and prostaglandin F2α (PGF2α), substance P (SP) in serum of rats with primary dysmenorrhea. Methods:A total of 45 non-pregnant Wistar rats were randomly divided into a control group, a model group and a WAA group, 15 rats in each group. Rats in the model group and the WAA group received continuous abdominal subcutaneous injection of Diethylstilbestrol to establish dysmenorrhea rat models. On the first day after modeling, rats in the WAA group began to receive acupuncture on Point Lower 1 and Point Lower 2, once a day for 10 d. The control group and the model group didn’t receive any treatment. Writhing latencies and frequencies were recorded.β-EP and NO in uterus tissue homogenates and PGF2α, SP in serum were detected. Results:In the model group,β-EP and NO levels were the lowest among the groups, the serum PGF2α level was the highest, and serum SP level was the lowest. These measurements showed significantly difference between the model group and the control group (P<0.05). PGF2α in the WAA group was lower than that in the model group;β-EP, NO and SP levels were higher than those in the model group, with inter-group statistically significant differences (P<0.05). Conclusion: WAA may achieve analgesic effect through decreasing PGF2α, increasingβ-EP, NO and SP to relieve uterine cramps, increase blood flow and promote functional improvement.
