针灸推拿医学（英文版）
針灸推拿醫學（英文版）
침구추나의학（영문판）
JOURNAL OF ACUPUNCTURE AND TUINA SCIENCE
2015年
3期
141-145
,共5页
针刺疗法%电针%抑郁%海马%脑源性神经营养因子%雌二醇%大鼠
針刺療法%電針%抑鬱%海馬%腦源性神經營養因子%雌二醇%大鼠
침자요법%전침%억욱%해마%뇌원성신경영양인자%자이순%대서
Acupuncture Therapy%Electroacupuncture%Depression%Hippocampus%Brain-derived Neurotrophic Factor%Estradiol%Rats
目的：探讨电针百会、内关和三阴交穴对慢性抑郁模型大鼠血清雌二醇(estradiol, E2)和脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)水平及海马区BDNF表达的影响。方法：将30只雌性Wistar大鼠随机分为三组,包括正常对照组,模型组和电针组,每组10只。采用慢性中等程度不可预见性刺激(chronic unpredicted mild stress, CUMS,如冷水游泳、夹尾、电击足底等)结合孤养21 d造模。模型组和电针组大鼠每天随机应用9种不同的刺激结合孤养。造模结束后,电针组给予电针百会、内关、三阴交治疗,每天一次,共14 d；模型组给予同等程度束缚。血清 E2用放免法检测,血清 BDNF 用酶联免疫吸附试验法(enzyme-linked immunosorbent assay, ELISA)检测，海马BDNF的表达用免疫组化法。结果：应激结束后,与正常对照组比较,模型组和电针组糖水偏爱率显著下降(P＜0.01)，强迫游泳不动时间显著增加(P＜0.01)。此外，电针显著减少了大鼠的抑郁样行为(P＜0.01)。与正常对照组比较，模型组血清E2、BDNF水平及海马区BDNF的表达显著下降(P＜0.01, P＜0.01, P＜0.05)。与模型组比较,电针组血清BDNF和海马区BDNF表达显著增加(P＜0.05)，血清E2水平增加但差异无显著性(P＞0.05)。结论：E2和BDNF可能与慢性轻度不可预见性应激大鼠抑郁样行为有关。电针可能通过增加血清和海马BDNF的表达发挥抗抑郁作用。
目的：探討電針百會、內關和三陰交穴對慢性抑鬱模型大鼠血清雌二醇(estradiol, E2)和腦源性神經營養因子(brain-derived neurotrophic factor, BDNF)水平及海馬區BDNF錶達的影響。方法：將30隻雌性Wistar大鼠隨機分為三組,包括正常對照組,模型組和電針組,每組10隻。採用慢性中等程度不可預見性刺激(chronic unpredicted mild stress, CUMS,如冷水遊泳、夾尾、電擊足底等)結閤孤養21 d造模。模型組和電針組大鼠每天隨機應用9種不同的刺激結閤孤養。造模結束後,電針組給予電針百會、內關、三陰交治療,每天一次,共14 d；模型組給予同等程度束縳。血清 E2用放免法檢測,血清 BDNF 用酶聯免疫吸附試驗法(enzyme-linked immunosorbent assay, ELISA)檢測，海馬BDNF的錶達用免疫組化法。結果：應激結束後,與正常對照組比較,模型組和電針組糖水偏愛率顯著下降(P＜0.01)，彊迫遊泳不動時間顯著增加(P＜0.01)。此外，電針顯著減少瞭大鼠的抑鬱樣行為(P＜0.01)。與正常對照組比較，模型組血清E2、BDNF水平及海馬區BDNF的錶達顯著下降(P＜0.01, P＜0.01, P＜0.05)。與模型組比較,電針組血清BDNF和海馬區BDNF錶達顯著增加(P＜0.05)，血清E2水平增加但差異無顯著性(P＞0.05)。結論：E2和BDNF可能與慢性輕度不可預見性應激大鼠抑鬱樣行為有關。電針可能通過增加血清和海馬BDNF的錶達髮揮抗抑鬱作用。
목적：탐토전침백회、내관화삼음교혈대만성억욱모형대서혈청자이순(estradiol, E2)화뇌원성신경영양인자(brain-derived neurotrophic factor, BDNF)수평급해마구BDNF표체적영향。방법：장30지자성Wistar대서수궤분위삼조,포괄정상대조조,모형조화전침조,매조10지。채용만성중등정도불가예견성자격(chronic unpredicted mild stress, CUMS,여랭수유영、협미、전격족저등)결합고양21 d조모。모형조화전침조대서매천수궤응용9충불동적자격결합고양。조모결속후,전침조급여전침백회、내관、삼음교치료,매천일차,공14 d；모형조급여동등정도속박。혈청 E2용방면법검측,혈청 BDNF 용매련면역흡부시험법(enzyme-linked immunosorbent assay, ELISA)검측，해마BDNF적표체용면역조화법。결과：응격결속후,여정상대조조비교,모형조화전침조당수편애솔현저하강(P＜0.01)，강박유영불동시간현저증가(P＜0.01)。차외，전침현저감소료대서적억욱양행위(P＜0.01)。여정상대조조비교，모형조혈청E2、BDNF수평급해마구BDNF적표체현저하강(P＜0.01, P＜0.01, P＜0.05)。여모형조비교,전침조혈청BDNF화해마구BDNF표체현저증가(P＜0.05)，혈청E2수평증가단차이무현저성(P＞0.05)。결론：E2화BDNF가능여만성경도불가예견성응격대서억욱양행위유관。전침가능통과증가혈청화해마BDNF적표체발휘항억욱작용。
Objective:To investigate the effects of electroacupuncture (EA) at acupoints Baihui (GV 20), Neiguan (PC 6) and Sanyinjiao (SP 6) on the levels of estradiol (E2) and brain-derived neurotrophic factor (BDNF) in serum, as well as the expression of BDNF in hippocampus in chronic depression rat models. Methods:Thirty female Wistar rats were randomly divided into three groups, including a normal control (NC) group, a model group and an EA group, 10 rats in each group. The depression model was established by using chronic unpredicted mild stress (CUMS), such as cold-water swimming, tail clamping, electric shock to foot, etc., combined with individual caging for 21 d. Rats in the model group and EA group were randomly exposed to one of the 9 stressors each day and caged individually. After modeling, rats in the EA group were then treated with EA at Baihui (GV 20), Neiguan (PC 6) and Sanyinjiao (SP 6) once daily for 14 d, and the model group was not treated with EA but bounded in the same way as the EA group. Serum E2 was measured by radioimmunoassay and BDNF was assessed by an enzyme-linked immunosorbent assay (ELISA) and the expression of BDNF in hippocampus was detected by using immunohistochemistry. Results:After the stress stimulation, compared with the NC group, the model group and EA group showed a significant reduction of sucrose preference rate (P<0.01) and remarkable increase of forced-swimming immobility time (P<0.01). In addition, EA significantly reduced the depression-like behavior of rats in the EA group (P<0.01). The expressions of E2 and BDNF in serum as well as the expression of BDNF in hippocampus were remarkably lower in the model rats than those in the NC group (P<0.01,P<0.01,P<0.05). The expression of BDNF in rats’ serum and hippocampus in the EA group was significantly higher than that in the model group (P<0.05), while serum E2 increased but insignificantly (P>0.05). Conclusion:E2 and BDNF may contribute to the depression-like behaviors of the rats during CUMS period. EA may exert its anti-depression effects through promoting BDNF expression in serum and hippocampus.
