现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
10期
1445-1447
,共3页
L-乳酸脱氢酶%植物蛋白质类%高温,诱发%反复冻融%脱水
L-乳痠脫氫酶%植物蛋白質類%高溫,誘髮%反複凍融%脫水
L-유산탈경매%식물단백질류%고온,유발%반복동융%탈수
Lactate dehydrogenases%Plant proteins%Hyperthermia,induced%Multigelation%Dehydration
目的：确定胚胎发育晚期丰富蛋白（LEA蛋白）在体外不同压力胁迫下对酶活性的保护作用，并对其可能机制进行探讨。方法评估OsLEA5蛋白对于防止乳酸脱氢酶（LDH）免受高温（43℃）、反复冻融和干燥脱水伤害的保护能力。磷酸盐缓冲液、牛血清蛋白（BSA）和纯水分别用于阳性和阴性对照。结果 OsLEA5蛋白组相对活性百分比在热处理10、20、30 min后分别是纯水对照组的2.2、3.7、5.4倍。在5个反复冻融循环后，纯水对照组的LDH活性降至7%甚至更低，而质量比2∶1和5∶1的OsLEA5蛋白组分别为28%和69%，质量比为10∶1组其活性几乎未受到影响。经过干燥处理后，不同质量比组LDH活性均有明显降低，其中纯水对照组和磷酸盐缓冲液组的LDH接近完全失活。质量比为5∶1和10∶1的OsLEA5蛋白组相对活性分别达24%和36%。结论 OsLEA5蛋白能够在各种环境胁迫下有效保护底物酶的体外活性或防止失活。
目的：確定胚胎髮育晚期豐富蛋白（LEA蛋白）在體外不同壓力脅迫下對酶活性的保護作用，併對其可能機製進行探討。方法評估OsLEA5蛋白對于防止乳痠脫氫酶（LDH）免受高溫（43℃）、反複凍融和榦燥脫水傷害的保護能力。燐痠鹽緩遲液、牛血清蛋白（BSA）和純水分彆用于暘性和陰性對照。結果 OsLEA5蛋白組相對活性百分比在熱處理10、20、30 min後分彆是純水對照組的2.2、3.7、5.4倍。在5箇反複凍融循環後，純水對照組的LDH活性降至7%甚至更低，而質量比2∶1和5∶1的OsLEA5蛋白組分彆為28%和69%，質量比為10∶1組其活性幾乎未受到影響。經過榦燥處理後，不同質量比組LDH活性均有明顯降低，其中純水對照組和燐痠鹽緩遲液組的LDH接近完全失活。質量比為5∶1和10∶1的OsLEA5蛋白組相對活性分彆達24%和36%。結論 OsLEA5蛋白能夠在各種環境脅迫下有效保護底物酶的體外活性或防止失活。
목적：학정배태발육만기봉부단백（LEA단백）재체외불동압력협박하대매활성적보호작용，병대기가능궤제진행탐토。방법평고OsLEA5단백대우방지유산탈경매（LDH）면수고온（43℃）、반복동융화간조탈수상해적보호능력。린산염완충액、우혈청단백（BSA）화순수분별용우양성화음성대조。결과 OsLEA5단백조상대활성백분비재열처리10、20、30 min후분별시순수대조조적2.2、3.7、5.4배。재5개반복동융순배후，순수대조조적LDH활성강지7%심지경저，이질량비2∶1화5∶1적OsLEA5단백조분별위28%화69%，질량비위10∶1조기활성궤호미수도영향。경과간조처리후，불동질량비조LDH활성균유명현강저，기중순수대조조화린산염완충액조적LDH접근완전실활。질량비위5∶1화10∶1적OsLEA5단백조상대활성분별체24%화36%。결론 OsLEA5단백능구재각충배경협박하유효보호저물매적체외활성혹방지실활。
Objective To determine the protective effect of late embryogenesis abundant (LEA) protein on enzyme ac-tivity under different stresses in vitro and discuss its potential mechanism. Methods The protective capability of OsLEA5 pro-teins to prevent lactic dehydrogenase(LDH) from high temperature(43℃),multigelation and dehydration was tested. Phosphate buffer,BSA and pure water were respectively used as positive and negative controls. Results The percentage of OsLEA5 proteins′relative activity was 2.2,3.7,5.4 times higher than pure water′s after 10,20,30 min of heat treatment. Pure water′s LDH activity dropped to 7%or even lower after 5 multigelation cycles while OsLEA5 proteins with a mass ratio of 2:1 and 5:1 were 28%and 69%,and OsLEA5 protein with a mass ratio of 10∶1 remained uninfluenced. The LDH activity of different mass ratios clearly dropped after drying treatment,in which pure water and phosphate buffer′s LDH was almost completely inactivated,and OsLEA5 proteins with a mass ratio of 5∶1 and 10∶1 was 24%and 36%. Conclusion OsLEA5 proteins effectively protect substrate enzyme′s activity in vitro and prevent inactivation under various stress conditions.
