CAJ | 학술논문

目的：探索在体外培养和纯化SD大鼠的骨髓间充质干细胞（BMSCs）的方法。方法：采用改良全骨髓贴壁方法，分离和纯化3～4周的大鼠BMSCs，镜下连续观察细胞的形态变化。流式细胞仪鉴定其表面抗原CD11b、CD45和CD90的表达情况。结果：原代培养的细胞呈圆形和梭形等，24小时后大部分细胞均贴壁。8～10天可达80%～90%融合，纯化后传代周期为6～8天。流式细胞术鉴定表明CD11b和CD45阴性，CD90阳性。结论：改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞，是一种比较理想的分离培养方法。
목적：탐색재체외배양화순화SD대서적골수간충질간세포（BMSCs）적방법。방법：채용개량전골수첩벽방법，분리화순화3～4주적대서BMSCs，경하련속관찰세포적형태변화。류식세포의감정기표면항원CD11b、CD45화CD90적표체정황。결과：원대배양적세포정원형화사형등，24소시후대부분세포균첩벽。8～10천가체80%～90%융합，순화후전대주기위6～8천。류식세포술감정표명CD11b화CD45음성，CD90양성。결론：개량전골수첩벽법가유효분리배양대서적골수간충질간세포，시일충비교이상적분리배양방법。
Objective To explore the in vitro culture and purification of SD rat bone marrow mesenchymal stem cells (BMSCs) method.Methods Using improved the whole bone marrow adherent method, separation and purification of rat (for 3~ 4 weeks )of BMSCs, microscopically continuous observation of cell morphology change. To identification cells surface antigen CD11b, CD45 and CD90 expression using flow cytometry.Results The original generation of cultured cells is circular and spindle, after 24 hours, most of the cells are attached. 8~10 days later, the fusion of up to 80%~ 90%. After purification,extend the period is 6~ 8 days. Using flow cytometry assay, CD11b and CD45 were negative, CD90 is positive.ConclusionsImproved the whole bone marrow adherent method, which can effectively the isolation and culture of rat bone marrow mesenchymal stem cells, is an ideal method of cultivation.