热带亚热带植物学报
熱帶亞熱帶植物學報
열대아열대식물학보
JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY
2015年
3期
343-352
,共10页
韩世忠%高人%马红亮%尹云锋%司友涛%杨玉盛%陈仕东%陆建芳%刘功辉%李爱萍%郑群瑞
韓世忠%高人%馬紅亮%尹雲鋒%司友濤%楊玉盛%陳仕東%陸建芳%劉功輝%李愛萍%鄭群瑞
한세충%고인%마홍량%윤운봉%사우도%양옥성%진사동%륙건방%류공휘%리애평%정군서
土壤真菌%米槠天然林%PCR-DGGE%真菌多样性
土壤真菌%米櫧天然林%PCR-DGGE%真菌多樣性
토양진균%미저천연림%PCR-DGGE%진균다양성
Soil fungi%Castanopsis carlesiinature forest%PCR-DGGE%Fungal diversity
为了解中亚热带森林土壤的真菌多样性，利用PCR-DGGE分子指纹图谱技术对武夷山国家级自然保护区和建瓯万木林自然保护区内的米槠(Castanopsis carlesii)天然林土壤真菌进行研究。结果表明，中亚热带地区森林土壤真菌群落丰富，土壤真菌的多样性指数、丰富度指数和均匀度指数分别为3.37~3.80、26~29和0.977~0.984。经测序及同源性比对，真菌以担子菌类(Basidiomycota)最多，占总数的35.9%；其次为子囊菌类(Ascomycota)，占总数的15.4%；接合菌类(Zygomycota)和半知菌类(Deuteromycota)分别占总数的10.3%和7.7%。主成分分析表明，地点和土层深度共同影响真菌群落组成，其中由地点引起的真菌群落组成差异大于土层深度。土壤真菌多样性指数与土壤铵态氮呈显著正相关，与土壤碳氮比呈显著负相关；丰富度指数和均匀度指数与土壤因子间没有显著的相关性，因此，土壤碳、氮含量是影响土壤真菌群落结构的重要因子。
為瞭解中亞熱帶森林土壤的真菌多樣性，利用PCR-DGGE分子指紋圖譜技術對武夷山國傢級自然保護區和建甌萬木林自然保護區內的米櫧(Castanopsis carlesii)天然林土壤真菌進行研究。結果錶明，中亞熱帶地區森林土壤真菌群落豐富，土壤真菌的多樣性指數、豐富度指數和均勻度指數分彆為3.37~3.80、26~29和0.977~0.984。經測序及同源性比對，真菌以擔子菌類(Basidiomycota)最多，佔總數的35.9%；其次為子囊菌類(Ascomycota)，佔總數的15.4%；接閤菌類(Zygomycota)和半知菌類(Deuteromycota)分彆佔總數的10.3%和7.7%。主成分分析錶明，地點和土層深度共同影響真菌群落組成，其中由地點引起的真菌群落組成差異大于土層深度。土壤真菌多樣性指數與土壤銨態氮呈顯著正相關，與土壤碳氮比呈顯著負相關；豐富度指數和均勻度指數與土壤因子間沒有顯著的相關性，因此，土壤碳、氮含量是影響土壤真菌群落結構的重要因子。
위료해중아열대삼림토양적진균다양성，이용PCR-DGGE분자지문도보기술대무이산국가급자연보호구화건구만목림자연보호구내적미저(Castanopsis carlesii)천연림토양진균진행연구。결과표명，중아열대지구삼림토양진균군락봉부，토양진균적다양성지수、봉부도지수화균균도지수분별위3.37~3.80、26~29화0.977~0.984。경측서급동원성비대，진균이담자균류(Basidiomycota)최다，점총수적35.9%；기차위자낭균류(Ascomycota)，점총수적15.4%；접합균류(Zygomycota)화반지균류(Deuteromycota)분별점총수적10.3%화7.7%。주성분분석표명，지점화토층심도공동영향진균군락조성，기중유지점인기적진균군락조성차이대우토층심도。토양진균다양성지수여토양안태담정현저정상관，여토양탄담비정현저부상관；봉부도지수화균균도지수여토양인자간몰유현저적상관성，인차，토양탄、담함량시영향토양진균군락결구적중요인자。
In order to understand the soil fungal diversity in mid-subtropical forest, the soil fungal community structure ofCastanopsis carlesiinature forests in Wuyi Mountain and Jian’ou Wanmulin Nature Reserves at northern Fujian, eastern China was studied by PCR-DGGE molecular ifngerprint techniques. The results showed that the diversity index, richness index and evenness index of soil fungal in the two subtropical forests were 3.37–3.80, 26–29 and 0.977–0.984, respectively, indicating that the natural forest soil fungal communities were abundant. Thirty-nine fungi species were obtained by sequencing and homologous alignment, among which Basidiomycota were the most, accounting for 35.9%; followed by Ascomycota for 15.4%, then Zygomycota for 10.3% and Deuteromycota for 7.7%. Principal component analysis showed both soil depth and sample location were the major factors influencing fungal community composition, and the effect of location was bigger than soil depth. Correlation analysis showed that soil fungal diversity index had signiifcant positive correlation with ammonium nitrogen content and negative with carbon nitrogen ratio, nevertheless, the community richness index and evenness index showed no signiifcant relation with the soil factors. So, the carbon and nitrogen contents in soil were important factors affecting the soil fungal community structure.
