山西医药杂志
山西醫藥雜誌
산서의약잡지
SHANXI MEDICAL JOURNAL
2015年
10期
1091-1094
,共4页
赵建国%雷德桥%蒙德鹏%侯春林%林浩东%宗海洋%陈寅生%蔡雨卫
趙建國%雷德橋%矇德鵬%侯春林%林浩東%宗海洋%陳寅生%蔡雨衛
조건국%뢰덕교%몽덕붕%후춘림%림호동%종해양%진인생%채우위
大鼠 ,Sprague-Dawley%尿流动力学%荧光金%神经吻合%神经重塑%神经示踪%神经根电刺激
大鼠 ,Sprague-Dawley%尿流動力學%熒光金%神經吻閤%神經重塑%神經示蹤%神經根電刺激
대서 ,Sprague-Dawley%뇨류동역학%형광금%신경문합%신경중소%신경시종%신경근전자격
Rats,Sprague-Dawley%Urodynamics%Fluorescent gold%Nerve anastomosis%Neural remodeling%Nerve tracer%Nerve root stimulation
目的:同时重建SD大鼠膀胱的运动和感觉神经支配,建立和鉴定动物模型,为进一步研究排尿中枢重塑及机制奠定基础。方法45只雌性SD大鼠分为对照组(10只),神经根切断组(15只)和神经根吻合组(20只)。神经根切断组大鼠,将腰椎(L )4以下双侧脊神经前后根全部切断;神经根吻合组在切断脊神经根后,将双侧L 4神经前后根与骶椎(S )1相应神经根吻合;对照组不做手术处理。术后6个月,取各组大鼠分别行尿流动力学检测、神经根电刺激、神经吻合口甲苯胺蓝染色、盆神经节注射荧光金神经示踪染色和膀胱湿质量测量。结果神经根吻合组大鼠膀胱最大容量、残余尿量及膀胱湿质量均小于神经根切断组而大于对照组;神经根吻合组大鼠最大排尿压与对照组差异无统计学意义( P >0.05),但大于神经根切断组( P <0.05)。吻合神经根行电刺激可于膀胱检测到压力变化。神经吻合口甲苯胺蓝染色可见神经通过率达到54%。盆神经节内注射荧光金后,神经根吻合组可见L4脊髓节段双侧灰质荧光金染色,神经根切断组和对照组未在相应脊髓节段检测到荧光金染色。结论从形态和功能角度证明成功建立同时重建膀胱感觉及运动神经支配的大鼠动物模型,为进一步研究排尿中枢重塑及机制奠定基础。
目的:同時重建SD大鼠膀胱的運動和感覺神經支配,建立和鑒定動物模型,為進一步研究排尿中樞重塑及機製奠定基礎。方法45隻雌性SD大鼠分為對照組(10隻),神經根切斷組(15隻)和神經根吻閤組(20隻)。神經根切斷組大鼠,將腰椎(L )4以下雙側脊神經前後根全部切斷;神經根吻閤組在切斷脊神經根後,將雙側L 4神經前後根與骶椎(S )1相應神經根吻閤;對照組不做手術處理。術後6箇月,取各組大鼠分彆行尿流動力學檢測、神經根電刺激、神經吻閤口甲苯胺藍染色、盆神經節註射熒光金神經示蹤染色和膀胱濕質量測量。結果神經根吻閤組大鼠膀胱最大容量、殘餘尿量及膀胱濕質量均小于神經根切斷組而大于對照組;神經根吻閤組大鼠最大排尿壓與對照組差異無統計學意義( P >0.05),但大于神經根切斷組( P <0.05)。吻閤神經根行電刺激可于膀胱檢測到壓力變化。神經吻閤口甲苯胺藍染色可見神經通過率達到54%。盆神經節內註射熒光金後,神經根吻閤組可見L4脊髓節段雙側灰質熒光金染色,神經根切斷組和對照組未在相應脊髓節段檢測到熒光金染色。結論從形態和功能角度證明成功建立同時重建膀胱感覺及運動神經支配的大鼠動物模型,為進一步研究排尿中樞重塑及機製奠定基礎。
목적:동시중건SD대서방광적운동화감각신경지배,건립화감정동물모형,위진일보연구배뇨중추중소급궤제전정기출。방법45지자성SD대서분위대조조(10지),신경근절단조(15지)화신경근문합조(20지)。신경근절단조대서,장요추(L )4이하쌍측척신경전후근전부절단;신경근문합조재절단척신경근후,장쌍측L 4신경전후근여저추(S )1상응신경근문합;대조조불주수술처리。술후6개월,취각조대서분별행뇨류동역학검측、신경근전자격、신경문합구갑분알람염색、분신경절주사형광금신경시종염색화방광습질량측량。결과신경근문합조대서방광최대용량、잔여뇨량급방광습질량균소우신경근절단조이대우대조조;신경근문합조대서최대배뇨압여대조조차이무통계학의의( P >0.05),단대우신경근절단조( P <0.05)。문합신경근행전자격가우방광검측도압력변화。신경문합구갑분알람염색가견신경통과솔체도54%。분신경절내주사형광금후,신경근문합조가견L4척수절단쌍측회질형광금염색,신경근절단조화대조조미재상응척수절단검측도형광금염색。결론종형태화공능각도증명성공건립동시중건방광감각급운동신경지배적대서동물모형,위진일보연구배뇨중추중소급궤제전정기출。
Objective To establish and identify SD rat models whose bladder motor and sensory innervation was rebuilt .It laid the foundation for further study on micturition center remodeling and mechanisms .Methods Forty‐five female SD rats were randomly divided into a control group ( n =10) ,rhizotomy group ( n =15) and nerve root anastomosis group ( n=20) .For rats in rhizotomy group ,their spinal nerve roots following the L4 all around was cut .Rats in nerve root anastomosis group were treated as anastomosis of the bilateral L4 nerve root and S1 nerve root after cutting the spinal nerve roots .Rats in control group were treated without surgery .In six months after surgery ,rats were taken into urodynamic testing ,nerve root stimulation ,nerve anastomosis toluidine blue staining ,fluorescence gold pelvic ganglia nerve tracer dye and bladder wet weight measurements.Results Rats′bladder maximum capacity ,residual urine volume and bladder wet weight in nerve root anastomosis group were less than those in the rhizotomy group ,but more than those in control group .Rats′maximum voiding pres‐sure in nerve root anastomosis group was more than that in rhizotomy group , but there are no statistical differ‐ences between the control group and nerve root anastomosis group ( P >0 .05) .Electrical stimulation to nerve root anastomosis line can be used to detect the pressure change in the bladder . Nerve through rate could be checked by toluidine staining nerve anastomosis .After injection of fluorescent gold in pelvic ganglia ,gold fluores‐cence staining is visible in the L4 spinal cord gray matter in nerve root anastomosis group but not visible in rhizoto‐my group and in control group .Conclusion The SD Rat model ,whose bladder was reconstructed while motor and sensory innervation was rebuilt successfully ,was proved from form and function .It laid the foundation for further study on micturition center remodeling and mechanisms.