CAJ | 학술논문

为了解云南莲瓣兰(Cymbidium tortisepalum)的遗传多样性，利用SSR技术对32个莲瓣兰主栽品种进行遗传变异分析，并构建莲瓣兰栽培品种的指纹图谱。结果表明，筛选出的12对多态性高、稳定性好的引物共检测到95个等位基因，每对引物检测到4~18个等位基因，有效等位基因数(NE)为61.489，平均有效等位基因数(NA)为5.124，Shannon信息指数(I)和多态性信息含量(PIC)分别为0.806~2.624和0.789~0.953。12对引物中，以引物SSR03的等位基因数、NE、观测杂合度、I和PIC最高。32个品种在12对引物上都具有不同的特异性条带，可以彼此区别。从12对引物中筛选出3对核心引物SSR02、SSR03和SSR12构建了莲瓣兰主栽品种SSR分子指纹图谱，这3对核心引物组合即可鉴定32个莲瓣兰栽培品种。这为莲瓣兰的品种鉴定、遗传多样性分析和分子育种研究提供理论基础和技术支持。
위료해운남련판란(Cymbidium tortisepalum)적유전다양성，이용SSR기술대32개련판란주재품충진행유전변이분석，병구건련판란재배품충적지문도보。결과표명，사선출적12대다태성고、은정성호적인물공검측도95개등위기인，매대인물검측도4~18개등위기인，유효등위기인수(NE)위61.489，평균유효등위기인수(NA)위5.124，Shannon신식지수(I)화다태성신식함량(PIC)분별위0.806~2.624화0.789~0.953。12대인물중，이인물SSR03적등위기인수、NE、관측잡합도、I화PIC최고。32개품충재12대인물상도구유불동적특이성조대，가이피차구별。종12대인물중사선출3대핵심인물SSR02、SSR03화SSR12구건료련판란주재품충SSR분자지문도보，저3대핵심인물조합즉가감정32개련판란재배품충。저위련판란적품충감정、유전다양성분석화분자육충연구제공이론기출화기술지지。
In order to understand the genetic diversity ofCymbidium tortisepulum, the ifngerprints of 32C. tortisepulum cultivars were constructed by SSR. The results showed that there were 95 alleles were detected by selected 12 pairs of primers, the number of alleles selected by one primers ranged from 4 to 18, and the number of mean alleles (NA) and effective alleles (NE) were 61.489 and 5.124, respectively. The Shannon information index (I) and polymorphism information content values (PIC) of 32 cultivars ranged from 0.806 to 2.624, and 0.789 to 0.953, respectively. Among 12 paris of primer, the primer SSR03 was the highest inNA,NE, observed heterozygosity (HO), I andPIC. All of 32 cultivars had different speciifc bands by 12 pairs of primer. The SSR molecular ifngerprints of 32 cultivars were built by 3 core pairs of primers, including SSR02, SSR03 and SSR12. All of 32 cultivars could be distinguished each other by combined primers of SSR02 + SSR03 + SSR123. These would provide theoretical basis and technical support for studying in the identiifcation ofC. tortisepalumcultivars, genetic diversity analysis and molecular breeding.