胃肠病学
胃腸病學
위장병학
CHINESE JOURNAL OF GASTROENTEROLOGY
2015年
5期
278-282
,共5页
辣椒素%慢性萎缩性胃炎%治疗
辣椒素%慢性萎縮性胃炎%治療
랄초소%만성위축성위염%치료
Capsaicin%Chronic Atrophic Gastritis%Therapy
慢性萎缩性胃炎是一种常见的胃炎类型，与胃癌的发生密切相关，但目前药物治疗的效果不确切。目的：探讨辣椒素对大鼠慢性萎缩性胃炎的治疗作用。方法：采用脱氧胆酸钠+乙醇+氨水灌胃的方法建立慢性萎缩性胃炎大鼠模型，将44只存活的模型大鼠随机分为模型组、低剂量辣椒素干预组、中剂量辣椒素干预组、高剂量辣椒素干预组，后3组大鼠分别灌胃1 mg·kg-1·d-1、10 mg·kg-1·d-1、30 mg·kg-1·d-1辣椒素，共持续28 d。采用HE 染色评估胃黏膜慢性炎症反应和萎缩程度，ELISA 法检测胃组织胃蛋白酶原（ PG）Ⅰ、PGⅡ水平，蛋白质印迹法检测胃黏膜组织IKKβ、NF-κB、XIAP 蛋白表达。结果：模型组、低剂量、中剂量、高剂量辣椒素干预组之间的胃黏膜慢性炎症反应、萎缩程度、PGⅠ、PGⅡ水平、胃黏膜IKKβ、NF-κB、XIAP 蛋白表达相比差异均有统计学意义（ P <0.05）；随着辣椒素干预剂量的增加，慢性炎症和胃黏膜萎缩程度逐渐好转（ P <0.05），IKKβ、NF-κB、XIAP 蛋白表达呈下降趋势。结论：辣椒素对慢性萎缩性胃炎具有较好的疗效。
慢性萎縮性胃炎是一種常見的胃炎類型，與胃癌的髮生密切相關，但目前藥物治療的效果不確切。目的：探討辣椒素對大鼠慢性萎縮性胃炎的治療作用。方法：採用脫氧膽痠鈉+乙醇+氨水灌胃的方法建立慢性萎縮性胃炎大鼠模型，將44隻存活的模型大鼠隨機分為模型組、低劑量辣椒素榦預組、中劑量辣椒素榦預組、高劑量辣椒素榦預組，後3組大鼠分彆灌胃1 mg·kg-1·d-1、10 mg·kg-1·d-1、30 mg·kg-1·d-1辣椒素，共持續28 d。採用HE 染色評估胃黏膜慢性炎癥反應和萎縮程度，ELISA 法檢測胃組織胃蛋白酶原（ PG）Ⅰ、PGⅡ水平，蛋白質印跡法檢測胃黏膜組織IKKβ、NF-κB、XIAP 蛋白錶達。結果：模型組、低劑量、中劑量、高劑量辣椒素榦預組之間的胃黏膜慢性炎癥反應、萎縮程度、PGⅠ、PGⅡ水平、胃黏膜IKKβ、NF-κB、XIAP 蛋白錶達相比差異均有統計學意義（ P <0.05）；隨著辣椒素榦預劑量的增加，慢性炎癥和胃黏膜萎縮程度逐漸好轉（ P <0.05），IKKβ、NF-κB、XIAP 蛋白錶達呈下降趨勢。結論：辣椒素對慢性萎縮性胃炎具有較好的療效。
만성위축성위염시일충상견적위염류형，여위암적발생밀절상관，단목전약물치료적효과불학절。목적：탐토랄초소대대서만성위축성위염적치료작용。방법：채용탈양담산납+을순+안수관위적방법건립만성위축성위염대서모형，장44지존활적모형대서수궤분위모형조、저제량랄초소간예조、중제량랄초소간예조、고제량랄초소간예조，후3조대서분별관위1 mg·kg-1·d-1、10 mg·kg-1·d-1、30 mg·kg-1·d-1랄초소，공지속28 d。채용HE 염색평고위점막만성염증반응화위축정도，ELISA 법검측위조직위단백매원（ PG）Ⅰ、PGⅡ수평，단백질인적법검측위점막조직IKKβ、NF-κB、XIAP 단백표체。결과：모형조、저제량、중제량、고제량랄초소간예조지간적위점막만성염증반응、위축정도、PGⅠ、PGⅡ수평、위점막IKKβ、NF-κB、XIAP 단백표체상비차이균유통계학의의（ P <0.05）；수착랄초소간예제량적증가，만성염증화위점막위축정도축점호전（ P <0.05），IKKβ、NF-κB、XIAP 단백표체정하강추세。결론：랄초소대만성위축성위염구유교호적료효。
Background:Chronic atrophic gastritis( CAG)is a common type of gastritis and is closely related to the pathogenesis of gastric carcinoma,however,the efficacy of its drug treatment is not clear. Aims:To study the therapeutic effect of capsaicin on CAG in rats. Methods:CAG rat model was established by intragastric administration of sodium deoxycholate, ethanol and ammonium hydroxide. Forty-four survival model rats were randomly divided into CAG model group and low, moderate,high dose capsaicin groups,rats in the latter 3 groups were intragastrically administrated with 1 mg·kg-1 ·d-1 , 10 mg·kg-1 · d-1 ,30 mg · kg-1 · d-1 capsaicin,respectively,for 28 days. The inflammation and degree of atrophy of gastric mucosa were observed by HE staining. Gastric mucosal levels of pepsinogen( PG)Ⅰ,PGⅡ were determined by ELISA. The protein expressions of IKKβ,NF-κB,XIAP were determined by Western blotting. Results:The differences in inflammation and degree of atrophy of gastric mucosa,levels of PGⅠ,PGⅡ,protein expressions of IKKβ,NF-κB,XIAP were statistically significant among model group,low,moderate,high dose capsaicin groups( P <0. 05 ). With the increasing of capsaicin dose,inflammation and degree of atrophy of gastric mucosa were improved in a dose-dependent manner(P<0. 05),protein expressions of IKKβ,NF-κB,XIAP were decreased. Conclusions:Capsaicin is effective for the treatment of CAG.
