CAJ | 학술논문

取罗布麻（Apocynum venetum L.）无菌苗中的叶、茎作为外植体，研究了不同激素组合对愈伤组织及不定芽分化的影响。结果表明：在多个激素浓度组合的培养基中，罗布麻的叶和茎的愈伤组织诱导率均能够达到100%；但进行不定芽分化时，茎来源的愈伤组织在不同激素配比的培养基中有不定芽分化，最高分化率可达到56.7%。因此，在构建罗布麻再生体系时，选择罗布麻茎段作为外植体，在培养基MS+6-BA 1.0mg/L+NAA 0.2mg/L中、光强2500lx，每天光照时间12h、25±3℃的培养条件下诱导愈伤组织，并转接到同样的培养基中进行不定芽分化的诱导，最后在1/2MS培养基中加入适量的NAA进行生根培养。
취라포마（Apocynum venetum L.）무균묘중적협、경작위외식체，연구료불동격소조합대유상조직급불정아분화적영향。결과표명：재다개격소농도조합적배양기중，라포마적협화경적유상조직유도솔균능구체도100%；단진행불정아분화시，경래원적유상조직재불동격소배비적배양기중유불정아분화，최고분화솔가체도56.7%。인차，재구건라포마재생체계시，선택라포마경단작위외식체，재배양기MS+6-BA 1.0mg/L+NAA 0.2mg/L중、광강2500lx，매천광조시간12h、25±3℃적배양조건하유도유상조직，병전접도동양적배양기중진행불정아분화적유도，최후재1/2MS배양기중가입괄량적NAA진행생근배양。
This paper takes the leaves, stems of apocynum of no vaccine as explants to study the effect of different hormones on callus and adventitious bud differentiation. The results showed that a combination of hormone concentrations in multiple media, callus induction rate of apocynum leaves and stems could reach 100%;but when adventitious buds, stems sources callus bud differentiation in culture has different hormone combinations, the highest differentiation rate reached 56.7%. Therefore, when constructing apocynum regeneration system, apocynum stem segments were selected as explants, in the medium MS+6-BA 1.0mg/L+NAA 0.2mg/L, the light intensity 2500lx, day light time 12h, 25 callus culture conditions ± 3 ℃ and transferred to the same medium to induce differentiation of adventitious buds. In the moderate NAA addition of 1/2MSm medium, it was cultured by rooting.