国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
10期
1410-1411
,共2页
耿雪芹%姜斌%周军兵%黄宏亮
耿雪芹%薑斌%週軍兵%黃宏亮
경설근%강빈%주군병%황굉량
献血者%丙型肝炎病毒%抗-HCV%筛查
獻血者%丙型肝炎病毒%抗-HCV%篩查
헌혈자%병형간염병독%항-HCV%사사
blood donor%hepatitis C virus%anti hepatitis C virus%screening
目的:了解盐城地区献血者丙型肝炎病毒(HCV )感染情况。方法采用酶联免疫吸附法(ELISA )检测献血者抗‐HCV ,用荧光定量聚合酶链反应检测 HCV RNA。结果盐城地区献血者抗‐HCV阳性率为0.07%(109/163782);109例抗‐HCV阳性者中,80例为单试剂阳性,29例为双试剂阳性;初次献血者抗‐HCV阳性80例,重复献血者抗‐HCV阳性29例;符合核酸检测要求的72例单试剂抗‐HCV阳性者均为 HCV RNA阴性。结论盐城地区献血者抗‐HCV阳性率低于普通人群,近三年献血者抗‐HCV阳性率没有变化,抗‐HCV单试剂阳性者核酸检测均为阴性。
目的:瞭解鹽城地區獻血者丙型肝炎病毒(HCV )感染情況。方法採用酶聯免疫吸附法(ELISA )檢測獻血者抗‐HCV ,用熒光定量聚閤酶鏈反應檢測 HCV RNA。結果鹽城地區獻血者抗‐HCV暘性率為0.07%(109/163782);109例抗‐HCV暘性者中,80例為單試劑暘性,29例為雙試劑暘性;初次獻血者抗‐HCV暘性80例,重複獻血者抗‐HCV暘性29例;符閤覈痠檢測要求的72例單試劑抗‐HCV暘性者均為 HCV RNA陰性。結論鹽城地區獻血者抗‐HCV暘性率低于普通人群,近三年獻血者抗‐HCV暘性率沒有變化,抗‐HCV單試劑暘性者覈痠檢測均為陰性。
목적:료해염성지구헌혈자병형간염병독(HCV )감염정황。방법채용매련면역흡부법(ELISA )검측헌혈자항‐HCV ,용형광정량취합매련반응검측 HCV RNA。결과염성지구헌혈자항‐HCV양성솔위0.07%(109/163782);109례항‐HCV양성자중,80례위단시제양성,29례위쌍시제양성;초차헌혈자항‐HCV양성80례,중복헌혈자항‐HCV양성29례;부합핵산검측요구적72례단시제항‐HCV양성자균위 HCV RNA음성。결론염성지구헌혈자항‐HCV양성솔저우보통인군,근삼년헌혈자항‐HCV양성솔몰유변화,항‐HCV단시제양성자핵산검측균위음성。
Objective To study the infection status of hepatitis C virus (HCV) in blood donors .Methods anti‐HCV was detec‐ted by using enzyme linked immunosorbent assay (ELISA) .HCV RNA was detected by using fluorescence quantitative polymerase chain reaction .Results The positive rate of anti‐HCV in blood donors was 0 .07% (109/163 782) .Among 109 positive donors ,80 donors were anti‐HCV positive while only one reagent was used ,and 29 donors were anti‐HCV positive when two reagents were used .80 cases were anti‐HCV positive in first donors and 29 cases were anti‐HCV positive in repeated donors .Among the 80 donors who were anti‐HCV positive while only one reagent was used ,72 samples according with the demand of nucleic acid test were tested by the nucleic acid test ,of whom HCV RNA was negative .Conclusion The positive rate of anti‐HCV in Yancheng donors could be lower than general population .There might be no change of positive rate of anti‐HCV in blood donors during the three years .The positive individuals could be negative in nucleic acid test while only one reagent was used in ELISA test .