CAJ | 학술논문

目的：分析2014年江苏南通地区肠道病毒71型（EV71）的分子流行病学特征。方法采集52例手足口病患儿咽拭子标本，提取病毒核酸，采用实时荧光定量PCR（RT‐PCR）扩增病毒的VP1序列。获得的序列与来自其他地区的序列进行比对分析，构建系统发育树。结果52份临床标本中分离到12株 EV71 VP1序列，12株 VP1序列的核苷酸、氨基酸同源性为93．4％～99．1％，进化树分析显示12株VP1序列全部属于C4基因型的C4a亚型。结论2014年南通地区分离的EV71流行病毒株与近年来中国其他地区的流行株有相同的起源，均属于EV71 C4基因的C4a亚型。
목적：분석2014년강소남통지구장도병독71형（EV71）적분자류행병학특정。방법채집52례수족구병환인인식자표본，제취병독핵산，채용실시형광정량PCR（RT‐PCR）확증병독적VP1서렬。획득적서렬여래자기타지구적서렬진행비대분석，구건계통발육수。결과52빈림상표본중분리도12주 EV71 VP1서렬，12주 VP1서렬적핵감산、안기산동원성위93．4％～99．1％，진화수분석현시12주VP1서렬전부속우C4기인형적C4a아형。결론2014년남통지구분리적EV71류행병독주여근년래중국기타지구적류행주유상동적기원，균속우EV71 C4기인적C4a아형。
Objective To analyze enterovirus 71 (EV71) molecular epidemiology characteristics in Nantong region of Jiangsu , 2014 .Methods The pharyngeal swab samples were selected from 52 children with hand ,foot and mouth disease .After extracting the virus nucleic acid ,the EV71 VP1 gene of the virus were amplified by RT‐PCR amplification .The phylogenetic tree was con‐structed between isolated and EV71 reference strains from other parts of the country .Results 12 strains EV71 VP1 gene were iso‐lated from 52 cases of clinical specimens .The nucleotide and amino acid homology of 12 strains EV71 VP1 gene was 93 .4% -99 .1% ,the phylogenetic tree analysis showed that 12 strains of VP1 gene all belong to C4 genotype C4a subgenotypes .Conclusion The isolated strains in Nantong in 2014 are all the EV71 C4a subgenotypes of C4 genotype ,the same with the most of isolates in re‐cent years .