国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
10期
1344-1345,1348
,共3页
全身型幼年特发性关节炎%自身抗体%炎性细胞因子
全身型幼年特髮性關節炎%自身抗體%炎性細胞因子
전신형유년특발성관절염%자신항체%염성세포인자
system-onset juvenile idiopathic arthritis%autoantibodies%inflammatory cytokines
目的：探讨自身抗体和炎性细胞因子在全身型幼年特发性关节炎（So‐JIA ）中的变化。方法分别采用间接免疫荧光法和酶联免疫吸附法检测活动期、缓解期So‐JIA患儿血清抗角蛋白抗体（AKA）、抗环瓜氨酸肽抗体（抗CCP抗体）、白细胞介素‐17（IL‐17）和白细胞介素‐6（IL‐6）的表达水平。以健康儿童作为健康对照组进行比较。结果活动期组血清AKA、抗CCP抗体阳性率分别为29．4％、47．1％，缓解期组分别为17．2％、27．6％，活动期组均高于缓解期组（P＜0．05）。So‐JIA组AKA的灵敏度和特异度分别为21．1％和97．2％，抗CCP抗体的灵敏度和特异度分别为41．3％和91．9％，抗CCP抗体的灵敏度高于AKA。活动期组抗CCP抗体、IL‐17、IL‐6水平均高于健康对照组和缓解期组（P＜0．05）；缓解期组抗CCP抗体、IL‐17、IL‐6水平高于健康对照组，但比较差异无统计学意义（P＞0．05）。结论自身抗体AKA和抗CCP抗体对So‐JIA具有较高的特异度，但灵敏度欠佳，二者联合检测对提高So‐JIA诊断特异度，尤其对提高活动期So‐JIA诊断阳性率有一定的临床价值。
目的：探討自身抗體和炎性細胞因子在全身型幼年特髮性關節炎（So‐JIA ）中的變化。方法分彆採用間接免疫熒光法和酶聯免疫吸附法檢測活動期、緩解期So‐JIA患兒血清抗角蛋白抗體（AKA）、抗環瓜氨痠肽抗體（抗CCP抗體）、白細胞介素‐17（IL‐17）和白細胞介素‐6（IL‐6）的錶達水平。以健康兒童作為健康對照組進行比較。結果活動期組血清AKA、抗CCP抗體暘性率分彆為29．4％、47．1％，緩解期組分彆為17．2％、27．6％，活動期組均高于緩解期組（P＜0．05）。So‐JIA組AKA的靈敏度和特異度分彆為21．1％和97．2％，抗CCP抗體的靈敏度和特異度分彆為41．3％和91．9％，抗CCP抗體的靈敏度高于AKA。活動期組抗CCP抗體、IL‐17、IL‐6水平均高于健康對照組和緩解期組（P＜0．05）；緩解期組抗CCP抗體、IL‐17、IL‐6水平高于健康對照組，但比較差異無統計學意義（P＞0．05）。結論自身抗體AKA和抗CCP抗體對So‐JIA具有較高的特異度，但靈敏度欠佳，二者聯閤檢測對提高So‐JIA診斷特異度，尤其對提高活動期So‐JIA診斷暘性率有一定的臨床價值。
목적：탐토자신항체화염성세포인자재전신형유년특발성관절염（So‐JIA ）중적변화。방법분별채용간접면역형광법화매련면역흡부법검측활동기、완해기So‐JIA환인혈청항각단백항체（AKA）、항배과안산태항체（항CCP항체）、백세포개소‐17（IL‐17）화백세포개소‐6（IL‐6）적표체수평。이건강인동작위건강대조조진행비교。결과활동기조혈청AKA、항CCP항체양성솔분별위29．4％、47．1％，완해기조분별위17．2％、27．6％，활동기조균고우완해기조（P＜0．05）。So‐JIA조AKA적령민도화특이도분별위21．1％화97．2％，항CCP항체적령민도화특이도분별위41．3％화91．9％，항CCP항체적령민도고우AKA。활동기조항CCP항체、IL‐17、IL‐6수평균고우건강대조조화완해기조（P＜0．05）；완해기조항CCP항체、IL‐17、IL‐6수평고우건강대조조，단비교차이무통계학의의（P＞0．05）。결론자신항체AKA화항CCP항체대So‐JIA구유교고적특이도，단령민도흠가，이자연합검측대제고So‐JIA진단특이도，우기대제고활동기So‐JIA진단양성솔유일정적림상개치。
Objective To investigate the changes of autoantibodies and inflammatory cytokines in children with system‐onset ju‐venile idiopathic arthritis (So‐JIA) .Methods Serum concentrations of autoantibodies and inflammatory cytokines were measured in children with So‐JIA at active phase or remission phase by using indirect immunofluorescence method and enzyme‐linked immu‐nosorbent assay .Healthy children were enrolled as control group .Results The assay positivity of antikeratin antibody (AKA) and anti‐cyclic citrullinated peptide antibody (anti‐CCP) was 29 .4% and 47 .1% in active phase group ,and was 17 .2% and 27 .6% in remission group .The differences between the two groups were significant (P<0 .05) .The diagnostic sensitivity of AKA and anti‐CCP was 21 .1% and 41 .3% ,and the diagnostic specificity was 97 .2% and 91 .9% .The diagnostic sensitivity of anti‐CCP was higher than AKA .Serum levels of anti‐CCP ,interleukin‐6 (IL‐6) and interleukin‐17 (IL‐17) of active phase group were higher than remission phase group and control group (P<0 .05) ,and those of remission phase group were higher than control group ,but the differences were not significant (P>0 .05) .Conclusion AKA and anti‐CCP might be with high diagnostic specificity to So‐JIA ,but the sensitivity could be low ,and combined detection could increase the diagnostic positivity .Inflammatory cytokines ,such as IL‐6 and IL‐17 ,might be associated with the activity of So‐JIA ,which could be important biomarkers of So‐JIA .
