浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2015年
5期
366-371
,共6页
曹羽%杨珺超%汪玉冠%夏永良%王媛
曹羽%楊珺超%汪玉冠%夏永良%王媛
조우%양군초%왕옥관%하영량%왕원
防感煎剂%甲型H1N1流感病毒%NO%iNOS%LYZ
防感煎劑%甲型H1N1流感病毒%NO%iNOS%LYZ
방감전제%갑형H1N1류감병독%NO%iNOS%LYZ
Fanggan decoction%influenza A virus type H1N1%NO%iNOS%LYZ
[目的]探讨防感煎剂对甲型H1N1流感病毒感染小鼠一氧化氮（nitric oxide，NO）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）以及溶菌酶（lysozyme，LYZ）的作用，进一步了解防感煎剂的疗效机理。[方法]选用清洁级雄性ICR小鼠96只，随机分为4组：正常对照组、模型对照组、防感煎剂组、达菲对照组。正常对照组和模型对照组用生理盐水灌胃，防感煎剂组按29．32g·kg－1剂量给予防感煎剂灌胃，达菲对照组按0．02g·kg－1剂量给予达菲胶囊溶液灌胃，各组给药1次/d。各组正常给药2d，第3d开始除正常对照组外均用甲型H1N1流感病毒液滴鼻造模，正常对照组用等量生理盐水滴鼻。给药持续到第8d，即造模后第5d，各组停止给药。造模后的第1、5、9、13d观察记录小鼠体重及一般情况，以上时间点每组随机处死6只小鼠，收集肺组织标本，RT－PCR检测感染小鼠流感病毒含量。取血分离血清，ELISA法检测小鼠血清NO、iNOS、LYZ含量。[结果]一般情况：模型对照组小鼠形体消瘦，毛色枯槁，精神萎靡、反应迟钝，饮食减少。防感煎剂组及达菲对照组小鼠症状明显较模型对照组减轻。体重：在第5、9、13d，防感煎剂组和达菲对照组小鼠平均体重均低于正常对照组（P<0．05），并且均高于模型对照组（P<0．01）。甲型H1N1流感病毒含量：第1、5、9、13d，防感煎剂组及达菲对照组小鼠肺组织病毒含量均低于模型对照组（P<0．05）。血清NO、iNOS、LYZ的含量：在第5d，防感煎剂组和达菲对照组小鼠血清NO、iNOS、LYZ含量明显高于模型对照组（P<0．05）及正常对照组（P<0．01），其余时间点差异无统计学意义（P＞0．05）。[结论]防感煎剂可抑制甲型H1N1流感病毒复制，增强NO、iNOS以及LYZ的表达，可能通过巨噬细胞环节，达到抵御或清除病毒的目的。
[目的]探討防感煎劑對甲型H1N1流感病毒感染小鼠一氧化氮（nitric oxide，NO）、誘導型一氧化氮閤酶（inducible nitric oxide synthase，iNOS）以及溶菌酶（lysozyme，LYZ）的作用，進一步瞭解防感煎劑的療效機理。[方法]選用清潔級雄性ICR小鼠96隻，隨機分為4組：正常對照組、模型對照組、防感煎劑組、達菲對照組。正常對照組和模型對照組用生理鹽水灌胃，防感煎劑組按29．32g·kg－1劑量給予防感煎劑灌胃，達菲對照組按0．02g·kg－1劑量給予達菲膠囊溶液灌胃，各組給藥1次/d。各組正常給藥2d，第3d開始除正常對照組外均用甲型H1N1流感病毒液滴鼻造模，正常對照組用等量生理鹽水滴鼻。給藥持續到第8d，即造模後第5d，各組停止給藥。造模後的第1、5、9、13d觀察記錄小鼠體重及一般情況，以上時間點每組隨機處死6隻小鼠，收集肺組織標本，RT－PCR檢測感染小鼠流感病毒含量。取血分離血清，ELISA法檢測小鼠血清NO、iNOS、LYZ含量。[結果]一般情況：模型對照組小鼠形體消瘦，毛色枯薧，精神萎靡、反應遲鈍，飲食減少。防感煎劑組及達菲對照組小鼠癥狀明顯較模型對照組減輕。體重：在第5、9、13d，防感煎劑組和達菲對照組小鼠平均體重均低于正常對照組（P<0．05），併且均高于模型對照組（P<0．01）。甲型H1N1流感病毒含量：第1、5、9、13d，防感煎劑組及達菲對照組小鼠肺組織病毒含量均低于模型對照組（P<0．05）。血清NO、iNOS、LYZ的含量：在第5d，防感煎劑組和達菲對照組小鼠血清NO、iNOS、LYZ含量明顯高于模型對照組（P<0．05）及正常對照組（P<0．01），其餘時間點差異無統計學意義（P＞0．05）。[結論]防感煎劑可抑製甲型H1N1流感病毒複製，增彊NO、iNOS以及LYZ的錶達，可能通過巨噬細胞環節，達到牴禦或清除病毒的目的。
[목적]탐토방감전제대갑형H1N1류감병독감염소서일양화담（nitric oxide，NO）、유도형일양화담합매（inducible nitric oxide synthase，iNOS）이급용균매（lysozyme，LYZ）적작용，진일보료해방감전제적료효궤리。[방법]선용청길급웅성ICR소서96지，수궤분위4조：정상대조조、모형대조조、방감전제조、체비대조조。정상대조조화모형대조조용생리염수관위，방감전제조안29．32g·kg－1제량급여방감전제관위，체비대조조안0．02g·kg－1제량급여체비효낭용액관위，각조급약1차/d。각조정상급약2d，제3d개시제정상대조조외균용갑형H1N1류감병독액적비조모，정상대조조용등량생리염수적비。급약지속도제8d，즉조모후제5d，각조정지급약。조모후적제1、5、9、13d관찰기록소서체중급일반정황，이상시간점매조수궤처사6지소서，수집폐조직표본，RT－PCR검측감염소서류감병독함량。취혈분리혈청，ELISA법검측소서혈청NO、iNOS、LYZ함량。[결과]일반정황：모형대조조소서형체소수，모색고고，정신위미、반응지둔，음식감소。방감전제조급체비대조조소서증상명현교모형대조조감경。체중：재제5、9、13d，방감전제조화체비대조조소서평균체중균저우정상대조조（P<0．05），병차균고우모형대조조（P<0．01）。갑형H1N1류감병독함량：제1、5、9、13d，방감전제조급체비대조조소서폐조직병독함량균저우모형대조조（P<0．05）。혈청NO、iNOS、LYZ적함량：재제5d，방감전제조화체비대조조소서혈청NO、iNOS、LYZ함량명현고우모형대조조（P<0．05）급정상대조조（P<0．01），기여시간점차이무통계학의의（P＞0．05）。[결론]방감전제가억제갑형H1N1류감병독복제，증강NO、iNOS이급LYZ적표체，가능통과거서세포배절，체도저어혹청제병독적목적。
Objective] To explore the influence of Fanggan Decoction on nitric oxide(NO) ,inducible nitric oxide synthase(iNOS) and lysozyme(LYZ) in mice infected with Influenza virus type 1. [Methods] 96 ICR male mice were randomly divided into four groups: normal control group ,model control group, Fanggan decoction group and Tamiflu control group. Normal control group and model control group were given saline with intragastric administration , Fanggan decoction group were treated with Fanggan decoction at dose of 29.32 g·kg-1,Tamiflu control group were treated with Tamiflu capsule solution at dose of 0.02 g·kg-1, each group administered one time per day. Each group administered for two days, on the 3rd day ,the H1N1 flu virus was made into droplets to nose to establish model expecting the normal control group with the same amount of saline. We stopped administering on the 8th day. The weight of mice and general condition were observed and recorded on the 1st, 5th, 9th, 13rd day after modelling. Six mice in each group was killed randomly on the same time point. Lung tissue samples were collected for the determination of H1N1 virus content by RT-PCR, while serum was collected for testing contents of NO,iNOS,LYZ by ELISA. [Results] General: mice in model control group were peaked, color haggard, apathetic, unresponsive, eating less, while mice in Fanggan decoction group and Tamiflu control group had less symptoms than those in the model control group. Weight: On the 5th,9th, 13rd day, the average weight of mice in Fanggan decoction group and Tamiflu control group was lower than those in the normal control group( P<0.05), and was higher than those in the model control group(P<0.01). H1N1 virus content: On the 1st,5th,9th,13rd day, mice lung tissue virus levels in Fanggan decoction group and Tamiflu control group were lower than those in the model control group ( P<0.05). Contents of NO, iNOS, LYZ in mice serum: On the 5th day, the contents of NO, iNOS, LYZ in mice serum in Fanggan decoction group and Tamiflu control group were significantly higher than those in the model control group(P<0.05) and normal control group(P<0.01), and the values on remaining time points had not significant change. [Conclusion] Fanggan decoction can resist or remove viruses by inhibiting replication of H1N1, and enhancing the expression of NO ,iNOS and LYZ, probably via macrophages pathway.
