浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2015年
5期
329-334
,共6页
张娟%马千%吕晨%赵莉%谢晓燕%郭小文
張娟%馬韆%呂晨%趙莉%謝曉燕%郭小文
장연%마천%려신%조리%사효연%곽소문
SGD%神经病理性疼痛(neuropathic pain, NP)%机械痛敏%热痛敏%CCI%SIRT1%NF-κB
SGD%神經病理性疼痛(neuropathic pain, NP)%機械痛敏%熱痛敏%CCI%SIRT1%NF-κB
SGD%신경병이성동통(neuropathic pain, NP)%궤계통민%열통민%CCI%SIRT1%NF-κB
Shayao Gancao decoction%neuropathic pain%mechanical withdrawal threshold%thermal withdrawal latency%CCI%SIRT1%NF-κB
[目的]观察芍药甘草汤（shaoyao－gancao decoction，SGD）对坐骨神经慢性压迫性损伤(chronic constriction injury，CCI)大鼠机械痛敏和热痛敏的影响，并研究SGD对CCI大鼠脊髓沉默信息调节因子2相关酶1（silent mating type information regulation 2 homolog 1, SIRT1）表达的影响。[方法] SD大鼠随机分为5组：假手术组（Sham组）、CCI组、SGD组、SGD+SIRT1－siRNA（small－interfering RNA，siRNA）和SGD+MM－siRNA（阴性对照）组，SGD组大鼠在CCI模型建立前6h开始灌胃给予40g·kg－1 SGD，1次/d，连续4d，而Sham组和CCI组大鼠则灌胃给予同体积的0．9%生理盐水；SGD+SIRT1－siRNA组大鼠除灌胃给予40g·kg－1 SGD，还鞘内给予10μL(2．5μg)的SIRT1－siRNA，SGD+MM－siRNA组则给予同剂量的MM－siRNA。各组分别在术前和术后第1、3、5、7d测定机械缩足反射阈值（mechanical withdrawal threshold，MWT）和热缩足潜伏期（thermal withdrawal latency， TWL），手术结束后提取大鼠脊髓测定SIRT1和Ac－NF－κB/NF－κB的蛋白表达。[结果]和Sham组相比，CCI组术后MWT及TWL均下降(P<0．05)， SGD组与CCI组相比，MWT和TWL在术后1、3和7d均明显增加(P<0．05)。和Sham组相比，CCI组SIRT1和Ac－NF－κB/NF－κB表达降低(P<0．01)，和CCI组比较，SGD+SIRT1－siRNA组SIRT1和Ac－NF－κB/NF－κB表达增加(P<0．01)，而鞘内预先给予SIRT1 siRNA组则可逆转SGM的作用(P<0．05，P<0．01)。[结论] SGD能抑制CCI大鼠的机械痛敏和热痛敏，其作用可能和增加脊髓SIRT1的表达有关。
[目的]觀察芍藥甘草湯（shaoyao－gancao decoction，SGD）對坐骨神經慢性壓迫性損傷(chronic constriction injury，CCI)大鼠機械痛敏和熱痛敏的影響，併研究SGD對CCI大鼠脊髓沉默信息調節因子2相關酶1（silent mating type information regulation 2 homolog 1, SIRT1）錶達的影響。[方法] SD大鼠隨機分為5組：假手術組（Sham組）、CCI組、SGD組、SGD+SIRT1－siRNA（small－interfering RNA，siRNA）和SGD+MM－siRNA（陰性對照）組，SGD組大鼠在CCI模型建立前6h開始灌胃給予40g·kg－1 SGD，1次/d，連續4d，而Sham組和CCI組大鼠則灌胃給予同體積的0．9%生理鹽水；SGD+SIRT1－siRNA組大鼠除灌胃給予40g·kg－1 SGD，還鞘內給予10μL(2．5μg)的SIRT1－siRNA，SGD+MM－siRNA組則給予同劑量的MM－siRNA。各組分彆在術前和術後第1、3、5、7d測定機械縮足反射閾值（mechanical withdrawal threshold，MWT）和熱縮足潛伏期（thermal withdrawal latency， TWL），手術結束後提取大鼠脊髓測定SIRT1和Ac－NF－κB/NF－κB的蛋白錶達。[結果]和Sham組相比，CCI組術後MWT及TWL均下降(P<0．05)， SGD組與CCI組相比，MWT和TWL在術後1、3和7d均明顯增加(P<0．05)。和Sham組相比，CCI組SIRT1和Ac－NF－κB/NF－κB錶達降低(P<0．01)，和CCI組比較，SGD+SIRT1－siRNA組SIRT1和Ac－NF－κB/NF－κB錶達增加(P<0．01)，而鞘內預先給予SIRT1 siRNA組則可逆轉SGM的作用(P<0．05，P<0．01)。[結論] SGD能抑製CCI大鼠的機械痛敏和熱痛敏，其作用可能和增加脊髓SIRT1的錶達有關。
[목적]관찰작약감초탕（shaoyao－gancao decoction，SGD）대좌골신경만성압박성손상(chronic constriction injury，CCI)대서궤계통민화열통민적영향，병연구SGD대CCI대서척수침묵신식조절인자2상관매1（silent mating type information regulation 2 homolog 1, SIRT1）표체적영향。[방법] SD대서수궤분위5조：가수술조（Sham조）、CCI조、SGD조、SGD+SIRT1－siRNA（small－interfering RNA，siRNA）화SGD+MM－siRNA（음성대조）조，SGD조대서재CCI모형건립전6h개시관위급여40g·kg－1 SGD，1차/d，련속4d，이Sham조화CCI조대서칙관위급여동체적적0．9%생리염수；SGD+SIRT1－siRNA조대서제관위급여40g·kg－1 SGD，환초내급여10μL(2．5μg)적SIRT1－siRNA，SGD+MM－siRNA조칙급여동제량적MM－siRNA。각조분별재술전화술후제1、3、5、7d측정궤계축족반사역치（mechanical withdrawal threshold，MWT）화열축족잠복기（thermal withdrawal latency， TWL），수술결속후제취대서척수측정SIRT1화Ac－NF－κB/NF－κB적단백표체。[결과]화Sham조상비，CCI조술후MWT급TWL균하강(P<0．05)， SGD조여CCI조상비，MWT화TWL재술후1、3화7d균명현증가(P<0．05)。화Sham조상비，CCI조SIRT1화Ac－NF－κB/NF－κB표체강저(P<0．01)，화CCI조비교，SGD+SIRT1－siRNA조SIRT1화Ac－NF－κB/NF－κB표체증가(P<0．01)，이초내예선급여SIRT1 siRNA조칙가역전SGM적작용(P<0．05，P<0．01)。[결론] SGD능억제CCI대서적궤계통민화열통민，기작용가능화증가척수SIRT1적표체유관。
Objective] To observe the effect and the SIRT1 expression of the extracts of the Chinese medicine shaoyao and gancao(SGD) on mechanical allodynia and heat hyperalgesia induced by chronic constriction injury(CCI) of the sciatic nerve in rats. [Methods] Mechanical allodynia and heat hyperalgesia were evaluated by mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) respectively. Rats were divided randomly into five groups for Sham group, CCI group, SGM group, SGD+MM-siRNA group and SGD+SIRT1-siRNA group. SGD was given to rats from day 0(6h before CCI surgery) to day 3 post-CCI surgery via gastric infusion, in dosage of 40g of crude drug/kg per day, 0.9%saline with the equal volume given to Sham and CCI groups. Mismatch siRNA and SIRT1 siRNA were given once daily, from the day before CCI to after CCI 3 days. The animals received intrathecal injections(2.5μg siRNA/10μL/rat) of either SIRT1-selective siRNA mixture(SGD+SIRT1-siRNA group) or i-Fect non-targeting dsRNA(SGD+MM-siRNA group). The TWL and MWT were measured before surgery and on days 1, 3, 5 and 7 after surgery. [Results] Compared with Sham group, rats with CCI significantly decreased PWL and PWT from the first day after surgery to the end of this study(P<0.01). Compared with CCI group, SGM group increased the MWT and TWL(P<0.05). In contrast to Sham group, remarkably decreased SIRT1 and Ac-NF-κB /NF-κB intensity was observed in the ipsilateral spinal cord of rats in the CCI group(P<0.01). SGD increased the CCI-induced SIRT1 and Ac-NF-κB /NF-κB expression levels in the spinal cord compared with the CCI group, which were reversed by SIRT1-siRNA(i.t)(P<0.05, P<0.01). [Conclusion] SGD attenuates behavioral hyperalgesia and allodynia in CCI rats by regulating the expression of SIRT1, which is related to decreasing Ac-NF-κB expression levels in the spinal cord.
