安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
6期
855-857
,共3页
周铭%唐海沁%蒋诗平%张勇
週銘%唐海沁%蔣詩平%張勇
주명%당해심%장시평%장용
血小板%钙分布%软X射线成像%谱学显微术
血小闆%鈣分佈%軟X射線成像%譜學顯微術
혈소판%개분포%연X사선성상%보학현미술
blood platelet%calcium distribution%soft X-ray imaging%microspectroscopy
探索性地应用软 X 射线谱学显微技术研究血小板的结构及钙元素成像的变化,采用谱学显微术对制取的 SD 大鼠血小板形态扫描透射成像并对血小板作钙元素双能对比衬度成像。血小板成像可看到血小板大小不等,有的伸出伪足;钙元素分布成像血小板在静息状态时胞内钙元素主要分布于质膜侧凹陷的钙池,诱导聚集后则扩散分布于胞质。软X 射线谱学显微成像能反应血小板钙元素分布,为钙的探测提供一种新方法。
探索性地應用軟 X 射線譜學顯微技術研究血小闆的結構及鈣元素成像的變化,採用譜學顯微術對製取的 SD 大鼠血小闆形態掃描透射成像併對血小闆作鈣元素雙能對比襯度成像。血小闆成像可看到血小闆大小不等,有的伸齣偽足;鈣元素分佈成像血小闆在靜息狀態時胞內鈣元素主要分佈于質膜側凹陷的鈣池,誘導聚集後則擴散分佈于胞質。軟X 射線譜學顯微成像能反應血小闆鈣元素分佈,為鈣的探測提供一種新方法。
탐색성지응용연 X 사선보학현미기술연구혈소판적결구급개원소성상적변화,채용보학현미술대제취적 SD 대서혈소판형태소묘투사성상병대혈소판작개원소쌍능대비츤도성상。혈소판성상가간도혈소판대소불등,유적신출위족;개원소분포성상혈소판재정식상태시포내개원소주요분포우질막측요함적개지,유도취집후칙확산분포우포질。연X 사선보학현미성상능반응혈소판개원소분포,위개적탐측제공일충신방법。
To test the transformation of intracellular calcium in platelet initially applying microspectroscopy soft X-ray for imaging based on the truth the platelets were at the status of activation in a variety of illness. Normal adult SD rats were enrolled in the trial and finally the rat platelets were extracted for platelet scanning transmission and dual-energy calcium contrast imaging. From the morphology and distribution of calcium of platelet,a various type of platelet morphology pictures were observed,of which the cell deformed and the parapodium stretched out;while calcium mainly distributed in the calcium pool near plasmalema under quiescent condition but scattered across the cytoplasm in activated condition induced by adenosine diphosphate. In conclusion,the microspectroscopy soft X-ray imaging was capable of reflecting the distribution of calcium in the platelet and provides a practicable new method for the measure of intracellular calcium.