CAJ | 학술논문

检测临床手术切除43例新疆维吾尔族结直肠癌患者癌组织及相应癌旁正常组织中微卫星不稳定（MSI）状态，探讨 MT1XT20基因与新疆维吾尔族 MSI 结直肠癌发生的关系。通过聚合酶链反应-单链构象多态性（PCR-SSCP）技术对新疆维吾尔族 MT1XT20基因与 MSI 结直肠癌检测及分析。在43例新疆维吾尔族患者中24例癌组织发生微卫星改变，总检出率为55.81%；8例发生一个微卫星位点改变，两个以上位点改变的有16例，癌旁正常组织中未检测到微卫星改变。在 MT1XT20位点上存在较高频率的微卫星改变，提示可能存在 MT1XT20基因与新疆维吾尔族 MSI 结直肠癌发病密切相关，为 MSI 结直肠癌的早期诊断提供了有意义的检测指标。
검측림상수술절제43례신강유오이족결직장암환자암조직급상응암방정상조직중미위성불은정（MSI）상태，탐토 MT1XT20기인여신강유오이족 MSI 결직장암발생적관계。통과취합매련반응-단련구상다태성（PCR-SSCP）기술대신강유오이족 MT1XT20기인여 MSI 결직장암검측급분석。재43례신강유오이족환자중24례암조직발생미위성개변，총검출솔위55.81%；8례발생일개미위성위점개변，량개이상위점개변적유16례，암방정상조직중미검측도미위성개변。재 MT1XT20위점상존재교고빈솔적미위성개변，제시가능존재 MT1XT20기인여신강유오이족 MSI 결직장암발병밀절상관，위 MSI 결직장암적조기진단제공료유의의적검측지표。
The microsatallite(MSI)stability of cancer and adjacent normal tissues was detected in 43 cases of Xinjiang Uygur patients with colorectal cancer after resection and the relationship between the MT1XT20 gene and the incidence of MSI colorectal cancer in Xinjiang Uygur people was discussed. The polymerase chain reaction -single strand conformation polymorphism( PCR-SSCP)technique was used for the detection and analysis of the Xinjiang Uygur MT1XT20 gene and MSI colorectal cancer. In 43 Xinjiang Uygur patients,24 cases had microsatel-lite alterations,with the total detection rate of 55. 81% ,8 cases sent a microsatellite alteration and 16 cases had more than two locus alterations. However,no microsatellite alteration was found in adjacent normal tissues. There was a high frequency of microsatellite alteration at the MT1XT20 locus,suggesting a close relationship between MT1XT20 gene and the incidence of colorectal cancer in Xinjiang Uygur people,thus providing a significant meas-ure for the early diagnosis of MSI colorectal cancer.