安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
6期
787-791,792
,共6页
任淑珍%史天陆%张蕾%姜玲%魏伟
任淑珍%史天陸%張蕾%薑玲%魏偉
임숙진%사천륙%장뢰%강령%위위
苍耳亭%SiHa细胞%增殖%凋亡
蒼耳亭%SiHa細胞%增殖%凋亡
창이정%SiHa세포%증식%조망
Xanthatin%SiHa cells%proliferation%apoptosis
目的:研究苍耳亭对宫颈癌鳞癌 SiHa 细胞增殖、凋亡及谷胱甘肽硫-转移酶 P1(GSTP1)的影响。方法 MTS法检测不同浓度苍耳亭对 SiHa 细胞生长的抑制作用,荧光显微术观察 SiHa 细胞形态的变化,流式细胞术检测苍耳亭对 SiHa 细胞凋亡的影响,酶标仪法检测 GSTP1酶活性,免疫组化检测 GSTP1蛋白表达,Western blot 法检测 p-JNK 蛋白表达。结果苍耳亭(0~40μmol/ L)对 SiHa 细胞的抑制作用呈时间-浓度依赖性,实验组 SiHa 细胞逐渐皱缩、变圆,甚至出现明显的凋亡小体。用不同浓度苍耳亭(0、5、10、20、40μmol/ L)处理 SiHa 细胞24 h 后,凋亡率分别为5.02%、9.62%、18.5%、26.4%和37.5%;与对照组相比,凋亡率明显升高。苍耳亭浓度性降低 SiHa 细胞 GSTP1酶活性及蛋白表达。Western blot 法结果显示苍耳亭可上调 SiHa 细胞 p-JNK 蛋白的表达。结论苍耳亭对宫颈癌 SiHa 细胞有明显的抑制增殖及促进凋亡作用,抑制 GSTP1、激活 c-Jun 氨基末端激酶(JNK)通路可能是其发挥诱导 SiHa 细胞凋亡的作用机制之一。
目的:研究蒼耳亭對宮頸癌鱗癌 SiHa 細胞增殖、凋亡及穀胱甘肽硫-轉移酶 P1(GSTP1)的影響。方法 MTS法檢測不同濃度蒼耳亭對 SiHa 細胞生長的抑製作用,熒光顯微術觀察 SiHa 細胞形態的變化,流式細胞術檢測蒼耳亭對 SiHa 細胞凋亡的影響,酶標儀法檢測 GSTP1酶活性,免疫組化檢測 GSTP1蛋白錶達,Western blot 法檢測 p-JNK 蛋白錶達。結果蒼耳亭(0~40μmol/ L)對 SiHa 細胞的抑製作用呈時間-濃度依賴性,實驗組 SiHa 細胞逐漸皺縮、變圓,甚至齣現明顯的凋亡小體。用不同濃度蒼耳亭(0、5、10、20、40μmol/ L)處理 SiHa 細胞24 h 後,凋亡率分彆為5.02%、9.62%、18.5%、26.4%和37.5%;與對照組相比,凋亡率明顯升高。蒼耳亭濃度性降低 SiHa 細胞 GSTP1酶活性及蛋白錶達。Western blot 法結果顯示蒼耳亭可上調 SiHa 細胞 p-JNK 蛋白的錶達。結論蒼耳亭對宮頸癌 SiHa 細胞有明顯的抑製增殖及促進凋亡作用,抑製 GSTP1、激活 c-Jun 氨基末耑激酶(JNK)通路可能是其髮揮誘導 SiHa 細胞凋亡的作用機製之一。
목적:연구창이정대궁경암린암 SiHa 세포증식、조망급곡광감태류-전이매 P1(GSTP1)적영향。방법 MTS법검측불동농도창이정대 SiHa 세포생장적억제작용,형광현미술관찰 SiHa 세포형태적변화,류식세포술검측창이정대 SiHa 세포조망적영향,매표의법검측 GSTP1매활성,면역조화검측 GSTP1단백표체,Western blot 법검측 p-JNK 단백표체。결과창이정(0~40μmol/ L)대 SiHa 세포적억제작용정시간-농도의뢰성,실험조 SiHa 세포축점추축、변원,심지출현명현적조망소체。용불동농도창이정(0、5、10、20、40μmol/ L)처리 SiHa 세포24 h 후,조망솔분별위5.02%、9.62%、18.5%、26.4%화37.5%;여대조조상비,조망솔명현승고。창이정농도성강저 SiHa 세포 GSTP1매활성급단백표체。Western blot 법결과현시창이정가상조 SiHa 세포 p-JNK 단백적표체。결론창이정대궁경암 SiHa 세포유명현적억제증식급촉진조망작용,억제 GSTP1、격활 c-Jun 안기말단격매(JNK)통로가능시기발휘유도 SiHa 세포조망적작용궤제지일。
Objective To investigate the effect of Xanthatin on proliferation,apoptosis and glutathione S-transferase P1(GSTP1)of cervical cancer SiHa cells. Methods MTS assay was used to study the effect of different concentra-tions of Xanthatin on the growth of SiHa cell lines. Morphological changes on SiHa cells were observed with fluores-cence microscope. The effect of Xanthatin on apoptosis of SiHa cells was analyzed by flow cytometry. In addition, changes of GSTP1 expression and activity were determined by immuohistochemistry and colorimetric method,and changes of p-JNK expression were determined by Western blot. Results Xanthatin(0 ~ 40 μmol/ L)significantly inhibited the proliferation on SiHa cells in a time and dose-dependent manner. The morphological changes observed in Xanthatin-treated cells included cell shrinkage,roundup and even some apoptotic bodies existed. After Xantha-tin treatment for 24 h in SiHa cells,the apoptotic ratios were 5. 02% ,9. 62% ,18. 5% ,26. 4% and 37. 5% with concentrations of Xanthatin at 0,5,10,20,40 μmol/ L. Compared with the control,apoptosis rate increased obvi-ously. SiHa cells exposed to increasing concentrations of Xanthatin led to the expression and activity of GSTP1 down-regulated and the expression of p-JNK. Conclusion These results demonstrate that Xanthatin exerts anti-tumor effects on SiHa cells through its anti-proliferative and pro-apoptotic roles. The molecular mechanism for pro-apoptotic effect may be related to its down-regulated effects on GSTP1 and up-regulated effects in c-Jun N-terminal kinase(JNK)pathway.