安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
6期
775-777,778
,共4页
沈彬%刘向辉%孙卫革%张磊%程义成%叶长林
瀋彬%劉嚮輝%孫衛革%張磊%程義成%葉長林
침빈%류향휘%손위혁%장뢰%정의성%협장림
纯钛%喷砂酸蚀%微弧氧化%成纤维细胞
純鈦%噴砂痠蝕%微弧氧化%成纖維細胞
순태%분사산식%미호양화%성섬유세포
pure titanium%sandblasting and acid etching%microarc oxidation%fibroblasts
将钛片随机分成光滑组、双重酸蚀喷砂组、氢氟酸酸蚀喷砂组、微弧氧化组,分别进行不同表面处理。将小鼠成纤维细胞接种于不同钛片表面,MTT 法检测成纤维细胞在不同钛片表面的黏附和增殖情况,并在培养24 h 后利用扫描电子显微镜观察成纤维细胞在试件表面的铺展形态。结果显示细胞黏附情况存在差异(P <0.05),光滑组<氢氟酸酸蚀喷砂组<双重酸蚀喷砂组<微弧氧化组;细胞增殖测试中,微弧氧化组在各时间点都显著高于另外3组。相较其他3种表面处理方法,成纤维细胞能够在经微弧氧化处理的试件表面更好地黏附、增殖。
將鈦片隨機分成光滑組、雙重痠蝕噴砂組、氫氟痠痠蝕噴砂組、微弧氧化組,分彆進行不同錶麵處理。將小鼠成纖維細胞接種于不同鈦片錶麵,MTT 法檢測成纖維細胞在不同鈦片錶麵的黏附和增殖情況,併在培養24 h 後利用掃描電子顯微鏡觀察成纖維細胞在試件錶麵的鋪展形態。結果顯示細胞黏附情況存在差異(P <0.05),光滑組<氫氟痠痠蝕噴砂組<雙重痠蝕噴砂組<微弧氧化組;細胞增殖測試中,微弧氧化組在各時間點都顯著高于另外3組。相較其他3種錶麵處理方法,成纖維細胞能夠在經微弧氧化處理的試件錶麵更好地黏附、增殖。
장태편수궤분성광활조、쌍중산식분사조、경불산산식분사조、미호양화조,분별진행불동표면처리。장소서성섬유세포접충우불동태편표면,MTT 법검측성섬유세포재불동태편표면적점부화증식정황,병재배양24 h 후이용소묘전자현미경관찰성섬유세포재시건표면적포전형태。결과현시세포점부정황존재차이(P <0.05),광활조<경불산산식분사조<쌍중산식분사조<미호양화조;세포증식측시중,미호양화조재각시간점도현저고우령외3조。상교기타3충표면처리방법,성섬유세포능구재경미호양화처리적시건표면경호지점부、증식。
Titanium plates were randomly divided into four groups,each with different surface treatments(smooth group,double acid corrosion sandblasting group,HF acid etching sandblasting group,microarc oxidation group). Mouse fibroblast cells were inoculated on different groups of titanium plates and tested by MTT to detect cells′ adhe-sion and proliferation. Cells were cultured for 24h and observed on the surface of plates by scanning electron micro-scope. The comparison between the rates of fibroblasts′ adhesion was smooth group < HF acid etching sandblasting group < double acid corrosion sandblasting group < microarc oxidation group(P < 0. 05). Fibroblasts′ proliferation was higher than on microarc oxidation group than those on the other three groups. Compared with the other three kinds of surface treatment,fibroblasts can be on the specimen surface treated by microarc oxidation better adhesion and proliferation.