CAJ | 학술논문

目的：检测 X 型胶原、基质金属蛋白酶13（MMP-13）在发育性髋关节发育不良（DDH）大鼠模型不同时期关节软骨中表达的改变情况，探讨 X 型胶原、MMP-13与软骨早期退变的相关性。方法选取新生 Wistar 大鼠80只，随机均等分成 DDH 组和对照组。DDH 组新生大鼠后肢襁褓位固定10 d 后解除固定继续饲养，分别于鼠的2、4、6、8周龄处死，离断髋关节，观察髋关节软骨的大体形态。用免疫组化和 qRT-PCR 法检测 X 型胶原、MMP-13的表达。结果 DDH模型鼠的尸体解剖样本显示关节囊增厚，髋臼表面呈不规则、不平整，股骨头扁平且发育较小，头臼包容差，尤其4周后更明显；与对照组相比，在髋关节软骨浅表区域有较高的X 型胶原和 MMP-13表达（P <0.05），并且这种增长是随着年龄增长而增长的，X 型胶原和 MMP-13的 mRNA 表达显示出相似的结果（P <0.05）。结论 DDH 模型鼠在较早阶段出现软骨退化，随着年龄逐渐加重；X 型胶原和 MMP-13表达与 DDH 软骨早期退变有密切的关系。
목적：검측 X 형효원、기질금속단백매13（MMP-13）재발육성관관절발육불량（DDH）대서모형불동시기관절연골중표체적개변정황，탐토 X 형효원、MMP-13여연골조기퇴변적상관성。방법선취신생 Wistar 대서80지，수궤균등분성 DDH 조화대조조。DDH 조신생대서후지강보위고정10 d 후해제고정계속사양，분별우서적2、4、6、8주령처사，리단관관절，관찰관관절연골적대체형태。용면역조화화 qRT-PCR 법검측 X 형효원、MMP-13적표체。결과 DDH모형서적시체해부양본현시관절낭증후，관구표면정불규칙、불평정，고골두편평차발육교소，두구포용차，우기4주후경명현；여대조조상비，재관관절연골천표구역유교고적X 형효원화 MMP-13표체（P <0.05），병차저충증장시수착년령증장이증장적，X 형효원화 MMP-13적 mRNA 표체현시출상사적결과（P <0.05）。결론 DDH 모형서재교조계단출현연골퇴화，수착년령축점가중；X 형효원화 MMP-13표체여 DDH 연골조기퇴변유밀절적관계。
Objective To detect the changes of expressions of X type collagen and matrix metalloproteinases-13 (MMP-13)of acetabular cartilage in the different stages of developmental dysplasia of the hip(DDH)and to inves-tigate the relevance between type X collagen,MMP-13 and the retrogression mechanism of acetabular cartilage. Methods 80 neonatal Wistar rats were randomly divided into DDH group(n = 40)and control group(n = 40). The DDH model was induced by fixation on both hips and knees for ten days and continued to be raised after relie-ving fixation. Those rats were sacrificed at age of 2,4,6,8 weeks respectively. The hips were isolated from the DDH model rats and an untreated control group for gross morphometry. Meanwhile,collagen X and matrix metallo-proteinase 13(MMP-13)were detected by quantitative RT-PCR and immunofluorescence. Results Necropsy spec-imens of DDH model rats showed thickening of the capsule. The acetabular surface was irregularly flat and had lost its smoothness. Flat and underdeveloped femoral head were observed as well. We also found the acetabulum and femoral head became smaller with poorer containment particularly after 4 weeks of age. Collagen X and MMP-13 ex-pressions were higher in the superficial zone of hip cartilage of DDH rats than in that of controls(P < 0. 05),and the increase was age-dependent. mRNA expression of Collagen X and MMP-13 showed similar results(P < 0. 05). Conclusion This study shows that degenerative cartilage changes occur at an early stage in the rats of the DDH model and become aggravated with age,which are closely associated with collagen X and MMP-13.