CAJ | 학술논문

目的：观察糖尿病视网膜病变发生过程中肾素原受体（PRR）及血管紧张素Ⅱ受体2（AT2R）的表达水平及视网膜神经细胞凋亡情况，阐明 PRR 与糖尿病视网膜神经细胞凋亡的关系。方法建立链尿佐菌素诱导的糖尿病 SD 大鼠模型，建模成功后分别在4周、8周和12周用免疫组化法检测 CD14及8-羟脱氧鸟苷分子的表达，检测糖尿病及对照组视网膜 AT2R、PRR mRNA 的表达及细胞外信号调节激酶（ERK1/2）、磷酸化 ERK1/2（p-ERK1/2）蛋白的表达。结果各病程 SD 大鼠视网膜 AT2R 和 PRR 的 mRNA 表达水平较对照组明显升高（P <0.05），随病程的延长表达量随之增多。病程越长糖尿病组大鼠视网膜组织凋亡细胞数量越多，各阶段病程 CD14染色未见明显新生血管。通过相关性分析显示，PRR 和 AT2R 表达水平与神经节细胞凋亡相关。各病程大鼠视网膜 ERK1/2、p-ERK1/2蛋白的表达量显著高于对照组。结论糖尿病视网膜病变中 PRR 和 AT2R 的表达与大鼠糖尿病视网膜病变的神经节细胞凋亡相关，可能通过增加 ERK 的磷酸化水平发挥功能作用。
목적：관찰당뇨병시망막병변발생과정중신소원수체（PRR）급혈관긴장소Ⅱ수체2（AT2R）적표체수평급시망막신경세포조망정황，천명 PRR 여당뇨병시망막신경세포조망적관계。방법건립련뇨좌균소유도적당뇨병 SD 대서모형，건모성공후분별재4주、8주화12주용면역조화법검측 CD14급8-간탈양조감분자적표체，검측당뇨병급대조조시망막 AT2R、PRR mRNA 적표체급세포외신호조절격매（ERK1/2）、린산화 ERK1/2（p-ERK1/2）단백적표체。결과각병정 SD 대서시망막 AT2R 화 PRR 적 mRNA 표체수평교대조조명현승고（P <0.05），수병정적연장표체량수지증다。병정월장당뇨병조대서시망막조직조망세포수량월다，각계단병정 CD14염색미견명현신생혈관。통과상관성분석현시，PRR 화 AT2R 표체수평여신경절세포조망상관。각병정대서시망막 ERK1/2、p-ERK1/2단백적표체량현저고우대조조。결론당뇨병시망막병변중 PRR 화 AT2R 적표체여대서당뇨병시망막병변적신경절세포조망상관，가능통과증가 ERK 적린산화수평발휘공능작용。
Objective Observing the pattern recognition receptor(PRR)and angiotensin Ⅱreceptors2(AT2R) expression level in rennin-angiotensin system,and retinal nerve cells apoptosis in the process of diabetic retinopa-thy,in order to explore the relationship between the PRR receptor and retinal nerve cells apoptosis in diabetic rats. Methods First,we built the diabetic rat model by using streptozocin. Second,the expressions of CD14 and 8-OHdG were detected by immunohistochemical. Third,the mRNA expressions of AT2R and PRR after 4,8 and 12 weeks of diabetes and normal control group were detected,respectively. Last,the levels of extracellular signal-reg-ulated kinase(ERK)1 / 2,p-ERK1 / 2 proteins were measured by Western blot. Results AT2R and PRR mRNA expression levels in diabetes groups were higher than the control group significantly,and the expression level was increasing with the extension of the course(P < 0. 05). The longer the course of the disease,the more rats retinal cells apoptosis in diabetes group. There were less new blood vessels in diabetes group as the CD14 dyeing was neg-tive. Through correlation analysis,PRR and AT2R expression levels were associated with the ganglion cell apopto-sis. In diabetes groups ERK1 / 2,p-ERK1 / 2 protein expressions were significantly higher than the control group. Conclusion AT2R and PRR mRNA expression levels are related with the cell apoptosis of rats with diabetic reti-nopathy. RAS may play a key role in diabetic retinopathy through increasing the phosphorylation of ERK level.