安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
6期
753-756
,共4页
马腾飞%王霁蕾%黄姗姗%秦健%韩良%赵利%肖芳莉%汪聪%王元银
馬騰飛%王霽蕾%黃姍姍%秦健%韓良%趙利%肖芳莉%汪聰%王元銀
마등비%왕제뢰%황산산%진건%한량%조리%초방리%왕총%왕원은
三叉神经痛%动物模型%炎症因子%脱髓鞘%眶下孔注射
三扠神經痛%動物模型%炎癥因子%脫髓鞘%眶下孔註射
삼차신경통%동물모형%염증인자%탈수초%광하공주사
trigeminal neuralgia%animal model%inflammatory cytokines%demyelination%infraorbital foramen in-jection
目的：经 SD 大鼠眶下孔注射炎症因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）以制造一种新型的大鼠三叉神经痛（TGN）模型。方法将大鼠随机分为4组，即生理盐水组（NS 组）、TNF-α组、IL-1β组、TNF-α+ IL-1β组；各组分别于大鼠眶下孔注射等量的生理盐水和 TNF-α或 IL-1β。观察并比较注射后不同时段各组大鼠注射侧触须垫机械痛阈、自发行为学改变以及电镜观察组织改变。结果从注射后3 d 至注射后8周，各炎症因子注射组大鼠均出现注射侧触须垫痛觉过敏及搔抓面部次数增多，同 NS 组相比，差异有统计学意义（P <0.05）；各炎症因子注射组间差异无统计学意义。各炎症因子注射组大鼠眶下神经及三叉神经节均出现不同程度的脱髓鞘改变，而 NS 组大鼠则无脱髓鞘现象。结论利用炎症因子 TNF-α、IL-1β经大鼠眶下孔注射，可使大鼠产生类似 TGN 症状和病理学变化，该 TGN 模型更接近于临床且造模方法简便。
目的：經 SD 大鼠眶下孔註射炎癥因子腫瘤壞死因子-α（TNF-α）、白細胞介素-1β（IL-1β）以製造一種新型的大鼠三扠神經痛（TGN）模型。方法將大鼠隨機分為4組，即生理鹽水組（NS 組）、TNF-α組、IL-1β組、TNF-α+ IL-1β組；各組分彆于大鼠眶下孔註射等量的生理鹽水和 TNF-α或 IL-1β。觀察併比較註射後不同時段各組大鼠註射側觸鬚墊機械痛閾、自髮行為學改變以及電鏡觀察組織改變。結果從註射後3 d 至註射後8週，各炎癥因子註射組大鼠均齣現註射側觸鬚墊痛覺過敏及搔抓麵部次數增多，同 NS 組相比，差異有統計學意義（P <0.05）；各炎癥因子註射組間差異無統計學意義。各炎癥因子註射組大鼠眶下神經及三扠神經節均齣現不同程度的脫髓鞘改變，而 NS 組大鼠則無脫髓鞘現象。結論利用炎癥因子 TNF-α、IL-1β經大鼠眶下孔註射，可使大鼠產生類似 TGN 癥狀和病理學變化，該 TGN 模型更接近于臨床且造模方法簡便。
목적：경 SD 대서광하공주사염증인자종류배사인자-α（TNF-α）、백세포개소-1β（IL-1β）이제조일충신형적대서삼차신경통（TGN）모형。방법장대서수궤분위4조，즉생리염수조（NS 조）、TNF-α조、IL-1β조、TNF-α+ IL-1β조；각조분별우대서광하공주사등량적생리염수화 TNF-α혹 IL-1β。관찰병비교주사후불동시단각조대서주사측촉수점궤계통역、자발행위학개변이급전경관찰조직개변。결과종주사후3 d 지주사후8주，각염증인자주사조대서균출현주사측촉수점통각과민급소조면부차수증다，동 NS 조상비，차이유통계학의의（P <0.05）；각염증인자주사조간차이무통계학의의。각염증인자주사조대서광하신경급삼차신경절균출현불동정도적탈수초개변，이 NS 조대서칙무탈수초현상。결론이용염증인자 TNF-α、IL-1β경대서광하공주사，가사대서산생유사 TGN 증상화병이학변화，해 TGN 모형경접근우림상차조모방법간편。
Objective To establish a rat model of trigeminal neuralgia by injecting inflammatory cytokines,TNF-αand IL-1β through infraorbital foramen. Methods Rats were randomly divided into four groups:normal saline group,TNF-α group,IL-1β group and TNF-α + IL-1β group. Rats in each group were injected with the same e-quivalent of normal saline and TNF-α or IL-1β,respectively. The mechanical withdrawal threshold of the injecting side of vibrissa pad,video detection of spontaneous behavior and the organizational change in electron microscopy were observed and compared in each group of rats. Results From 3 d to 8 w after injection,rats in all cytokines injecting groups appeared hyperalgesia in the vibrissa pad. And there was an increased frequency of scratching the face at the same time. Compared with the normal saline group,it was significantly different(P < 0. 05). However, there was not significant difference among TNF-α group,IL-1β group and TNF-α + IL-1β group. Nerve demyeli-nations were observed in both infraorbital nerve and trigeminal ganglion in all cytokines injecting group,while there was no demyelination in the normal saline group. Conclusion Injecting cytokines,TNF-α and / or IL-1β can make a trigeminal neuralgia model which is easy to operate and very close to the trigeminal neuralgia in clinic.
