CAJ | 학술논문

目的寻找淋巴瘤细胞特征性的气体标志物，为应用呼气监测对淋巴瘤患者的早期诊断提供实验依据，评估固相微萃取-气相色谱/质谱技术（SPME-GC/ MS）应用于淋巴瘤细胞顶空气体的检测的可行性。方法采用 SPME-GC/ MS检测非霍奇金淋巴瘤细胞株 JEOK 细胞在培养瓶顶空气体中挥发性有机物（VOCs）的成份，与人淋巴细胞株和空白对照组顶空气体中的 VOCs 比较，通过单样本秩和检验筛选出非霍奇金淋巴瘤细胞的特征性挥发性标志物。结果在JEOK 细胞培养瓶顶空气体中可检测到二甲基硫、甲苯、邻二甲苯、1，3-二叔丁基苯、苯乙酮、十二烷，较人淋巴细胞升高；而乙醇、苯甲醛、正己醛明显减少。结论非霍奇金淋巴瘤细胞株 JEOK 可以引起培养瓶顶空气体中 VOCs 组成的改变，芳香族化合物与烷烃类化合物增多，乙醇及醛类含量减少，上述物质可能作为淋巴瘤细胞特征性标志物；SPME-GC/MS 作为一种衡量物质检测方法，可以用于 JEOK 细胞株培养瓶顶空气体中 VOCs 的检测。
목적심조림파류세포특정성적기체표지물，위응용호기감측대림파류환자적조기진단제공실험의거，평고고상미췌취-기상색보/질보기술（SPME-GC/ MS）응용우림파류세포정공기체적검측적가행성。방법채용 SPME-GC/ MS검측비곽기금림파류세포주 JEOK 세포재배양병정공기체중휘발성유궤물（VOCs）적성빈，여인림파세포주화공백대조조정공기체중적 VOCs 비교，통과단양본질화검험사선출비곽기금림파류세포적특정성휘발성표지물。결과재JEOK 세포배양병정공기체중가검측도이갑기류、갑분、린이갑분、1，3-이숙정기분、분을동、십이완，교인림파세포승고；이을순、분갑철、정기철명현감소。결론비곽기금림파류세포주 JEOK 가이인기배양병정공기체중 VOCs 조성적개변，방향족화합물여완경류화합물증다，을순급철류함량감소，상술물질가능작위림파류세포특정성표지물；SPME-GC/MS 작위일충형량물질검측방법，가이용우 JEOK 세포주배양병정공기체중 VOCs 적검측。
Objective To find lymphoma cell markers characteristic of gas for breath monitoring application and provide experimental evidence for the early diagnosis of lymphoma patients,assessing solid phase micro extraction-gas chromatography / mass spectrometry( SPME-GC / MS)is applied to the lymphatic the feasibility of detecting tumor cells in the headspace. To investigate the value of VOCs in the diagnosis of hematologic malignancies. Meth-ods The air samples from the headspace of non-Hodgkin lymphoma cell JEOK,the human lymphoidcell lines and culture medium were collected by syringes,and then determined by means of SPME-GC / MS in order to have a bet-ter understanding of the concentration distributions and changes of VOCs in JEOK cells headspace. Using Mann-Whitney U test,we found the characteristic volatile markers of non-Hodgkin′s lymphoma cells. Results SPME-GC /MS for JEOK cell lines,human lymphocytecell line and a blank incubation were detected headspace analysis,found JEOK cell culture headspace can be detected dimethyl,sulfide,toluene,o-xylene,1,3-Di-tert-butylbenzene,aceto-pheno-ne,dodecane,representing human lymphocytes increased;and alcohol,benzaldehyde,hexanal is signifi-cantly reduced. Conclusion Non-Hodgkin′s lymphoma cell lines can cause flasks JEOK headspace change in the composition of VOCs,increased alkanes and aromatic compounds,aldehydes and reducing alcohol content,these substances may be used as acharacteristic of lymphoma cells markers;SPME-GC / MS as a trace substance detection method can be used to JEOK cell lines VOCs bottle headspace detection training.