安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
6期
730-734
,共5页
雷婷%秦宜德%刘琛%周娟%赵梦静
雷婷%秦宜德%劉琛%週娟%趙夢靜
뢰정%진의덕%류침%주연%조몽정
RNA干扰%ERCCl%卵巢癌%DDP%PGPIPN
RNA榦擾%ERCCl%卵巢癌%DDP%PGPIPN
RNA간우%ERCCl%란소암%DDP%PGPIPN
RNA interference%ERCCl%ovarian cancer%DDP%PGPIPN
目的:探讨 RNA 干扰核苷酸剪切修复偶联因子1(ERCCl)基因表达对人卵巢癌耐药细胞 DDP、PGPIPN 敏感性的影响。方法实验分为空白对照组、特异性转染组和非特异性转染组。采用 MTT 法测定 PGPIPN、DDP 对3组细胞的增殖抑制率,RT-PCR 法检测 PGPIPN 对3组细胞乳腺癌易感基因1(BRCAl)基因表达的影响。结果 MTT 法结果显示,PGPIPN 作用人卵巢癌耐药细胞 SKOV3/ DDP 48 h,对其增殖有一定的抑制作用,在浓度为40、60 mg/ L 时,与浓度为0 mg/ L 比较,差异有统计学意义( P <0.05);DDP、PG-PIPN 分别作用特异性转染组细胞48 h,其增殖明显受到抑制,与0 mg/ L 比较,差异均有统计学意义( P <0.05);RT-PCR 法结果显示,PGPIPN 作用特异性转染组细胞48 h,其BRCAl 基因表达随着 PGPIPN 浓度升高而明显降低,在浓度为40、60 mg/ L 时,与 PGPIPN 浓度为0 mg/ L 比较,差异有统计学意义(P <0.01)。结论 PGPIPN 对 SKOV3/ DDP 细胞的增殖有一定的抑制作用,并且通过干扰 ERCCl 基因表达可以明显增强 SKOV3/ DDP 细胞对 DDP、PGPIPN 的敏感性,同时可增强 PGPIPN 对 BRCAl 基因的下调水平。
目的:探討 RNA 榦擾覈苷痠剪切脩複偶聯因子1(ERCCl)基因錶達對人卵巢癌耐藥細胞 DDP、PGPIPN 敏感性的影響。方法實驗分為空白對照組、特異性轉染組和非特異性轉染組。採用 MTT 法測定 PGPIPN、DDP 對3組細胞的增殖抑製率,RT-PCR 法檢測 PGPIPN 對3組細胞乳腺癌易感基因1(BRCAl)基因錶達的影響。結果 MTT 法結果顯示,PGPIPN 作用人卵巢癌耐藥細胞 SKOV3/ DDP 48 h,對其增殖有一定的抑製作用,在濃度為40、60 mg/ L 時,與濃度為0 mg/ L 比較,差異有統計學意義( P <0.05);DDP、PG-PIPN 分彆作用特異性轉染組細胞48 h,其增殖明顯受到抑製,與0 mg/ L 比較,差異均有統計學意義( P <0.05);RT-PCR 法結果顯示,PGPIPN 作用特異性轉染組細胞48 h,其BRCAl 基因錶達隨著 PGPIPN 濃度升高而明顯降低,在濃度為40、60 mg/ L 時,與 PGPIPN 濃度為0 mg/ L 比較,差異有統計學意義(P <0.01)。結論 PGPIPN 對 SKOV3/ DDP 細胞的增殖有一定的抑製作用,併且通過榦擾 ERCCl 基因錶達可以明顯增彊 SKOV3/ DDP 細胞對 DDP、PGPIPN 的敏感性,同時可增彊 PGPIPN 對 BRCAl 基因的下調水平。
목적:탐토 RNA 간우핵감산전절수복우련인자1(ERCCl)기인표체대인란소암내약세포 DDP、PGPIPN 민감성적영향。방법실험분위공백대조조、특이성전염조화비특이성전염조。채용 MTT 법측정 PGPIPN、DDP 대3조세포적증식억제솔,RT-PCR 법검측 PGPIPN 대3조세포유선암역감기인1(BRCAl)기인표체적영향。결과 MTT 법결과현시,PGPIPN 작용인란소암내약세포 SKOV3/ DDP 48 h,대기증식유일정적억제작용,재농도위40、60 mg/ L 시,여농도위0 mg/ L 비교,차이유통계학의의( P <0.05);DDP、PG-PIPN 분별작용특이성전염조세포48 h,기증식명현수도억제,여0 mg/ L 비교,차이균유통계학의의( P <0.05);RT-PCR 법결과현시,PGPIPN 작용특이성전염조세포48 h,기BRCAl 기인표체수착 PGPIPN 농도승고이명현강저,재농도위40、60 mg/ L 시,여 PGPIPN 농도위0 mg/ L 비교,차이유통계학의의(P <0.01)。결론 PGPIPN 대 SKOV3/ DDP 세포적증식유일정적억제작용,병차통과간우 ERCCl 기인표체가이명현증강 SKOV3/ DDP 세포대 DDP、PGPIPN 적민감성,동시가증강 PGPIPN 대 BRCAl 기인적하조수평。
Objective To investigate the effects of gene expression of RNA interfering nucleotide excision repair cross-complementation 1( ERCCl)on DDP,PGPIPN sensitivity in human ovarian cancer drug resistance cell. Methods In the test,there were blank control group,specificity transfection group and non-specificity transfection group. MTT method was used to determine the effects of PGPIPN,DDP on cell proliferation inhibition rate for the three groups;RT-PCR method was used to test the effects of PGPIPN on gene expression of breast cancer suscepti-bility gene 1(BRCAl)in cell of the three groups. Results MTT results showed that PGPIPN affected human ovari-an cancer drug resistance cell for 48 h,and provided certain inhibition for their proliferation,which was statistically significant at 40,60 mg / L of concentration compared to 0 mg / L(P < 0. 05);DDP and PGPIPN affected cell for 48 h respectively in the specificity transfection group,in which the cell proliferation was significantly inhibited,both of which had statistically significant differences compared to 0 mg / L of DDP,PGPIPN concentration(P < 0. 05);RT-PCR results showed that PGPIPN affected cells for 48 h in specificity transfection group,BRCAl gene expression of which significantly decreased with the increase of PGPIPN concentration,having statistically significant differences at 40,60 mg / L of concentration compared to 0 mg / L of PGPIPN concentration(P < 0. 01). Conclusion PGPIPN has certain inhabitation effect on the proliferation of SKOV3 / DDP,and it can significantly enhance the DDP and PGPIPN sensitivity by interference ERCCl gene expression of SKOV3 / DDP. Meanwhile,it can enhance PGPIPN down-regulate the level of BRCAl gene expression.