CAJ | 학술논문

目的：探讨结肠癌组织中人软骨糖蛋白39（YKL-40）和白细胞介素-6（IL-6）蛋白的表达变化及其临床意义。方法采用 Western blot、qRT-PCR 法检测30例接受根治性手术治疗的结肠癌患者的结肠癌组织标本及结肠癌旁组织中 YKL-40和 IL-6的表达变化。分析患者 YKL-40和IL-6的表达与结肠癌的组织分化程度等临床病理学特征的关系。结果 Western blot 实验结果显示结肠癌组织中YKL-40和 IL-6蛋白表达明显高于结肠癌旁组织（ P <0.05），qRT-PCR 结果表明结肠癌组织中 YKL-40和 IL-6 mRNA 表达明显高于结肠癌旁组织（P <0.05）。结肠癌组织中 YKL-40与 IL-6表达呈正相关性（ r =0.812，P <0.05）。此外，YKL-40和 IL-6表达与 TNM 分期、分化程度相关（P <0.05）。结论 YKL-40和 IL-6在结肠癌组织中呈高表达，可能与结肠癌的发生、发展有关。
목적：탐토결장암조직중인연골당단백39（YKL-40）화백세포개소-6（IL-6）단백적표체변화급기림상의의。방법채용 Western blot、qRT-PCR 법검측30례접수근치성수술치료적결장암환자적결장암조직표본급결장암방조직중 YKL-40화 IL-6적표체변화。분석환자 YKL-40화IL-6적표체여결장암적조직분화정도등림상병이학특정적관계。결과 Western blot 실험결과현시결장암조직중YKL-40화 IL-6단백표체명현고우결장암방조직（ P <0.05），qRT-PCR 결과표명결장암조직중 YKL-40화 IL-6 mRNA 표체명현고우결장암방조직（P <0.05）。결장암조직중 YKL-40여 IL-6표체정정상관성（ r =0.812，P <0.05）。차외，YKL-40화 IL-6표체여 TNM 분기、분화정도상관（P <0.05）。결론 YKL-40화 IL-6재결장암조직중정고표체，가능여결장암적발생、발전유관。
Objective To observe the changes of expression of cartilage glycoprotein-39(YKL-40)and interleukin 6(IL-6)in colon cancer tissues. Methods Colon tissues were isolated from 30 colon cancer patients who had re-ceived radical surgeries normal persons. Western blot and qRT-PCR were used to detect the expression of YKL-40 and IL-6. Analyzing the relationship between the expression of YKL-40 and tumor differentiation,the depth of tumor invasion,lymph node metastases and other clinical pathological characteristics. Results Compared with the colon tissue beside tumor tissue,Western blot showed that the protein expressions of YKL-40 and IL-6 were signifi-cantly increased in the model group(P < 0. 05). qRT-PCR found that the mRNA expression of YKL-40 and IL-6 are significantly increased in the model group(P < 0. 05). The intensive expression of YKL-40 was positively cor-related to TNM stage and pathologic grade(P < 0. 05). Conclusion The expression of YKL-40 and IL-6 in colon cancer is high and this may have relationship with tumor progression.