CAJ | 학술논문

栽培种花生是异源四倍体,基因组大,构建花生的分子遗传连锁图谱并对相关性状进行 QTL 定位研究的工作缓慢。本研究以遗传差异大的亲本组配杂交组合富川大花生×ICG6375构建 F2作图群体,采用公开发表的2653对SSR引物,构建了一张含有234个SSR标记、分布于20个连锁群的栽培种花生遗传图谱。该图谱覆盖基因组的长度为1683.43 cM,各个连锁群长度在36.11~131.48 cM之间,每个连锁群的标记数在6~15个之间,标记间的平均距离为7.19 cM。结合F3在湖北武汉和阳逻环境下的主茎高和总分枝数鉴定结果,应用WinQTLCart 2.5软件采用复合区间作图法进行了QTL定位和遗传效应分析。共检测到17个与主茎高和总分枝数相关的QTL位点,贡献率在0.10%~10.22%之间,分布于8个连锁群上。综合分析武汉和阳逻环境的鉴定结果,获得重复一致的与主茎高相关的6个 QTL,其中 qMHA061.1和 qMHA062.1位于连锁群 LG06上 TC1A2~AHGS0153标记区间,贡献率为5.49%~8.95%； qMHA061.2和 qMHA062.2位于 LG06上 AHGS1375~PM377标记区间,贡献率为2.93%~5.83%； qMHA092.2和 qMHA091.1位于连锁群 LG09上GM2839~EM87标记区间,贡献率为0.53%~9.43%。
재배충화생시이원사배체,기인조대,구건화생적분자유전련쇄도보병대상관성상진행 QTL 정위연구적공작완만。본연구이유전차이대적친본조배잡교조합부천대화생×ICG6375구건 F2작도군체,채용공개발표적2653대SSR인물,구건료일장함유234개SSR표기、분포우20개련쇄군적재배충화생유전도보。해도보복개기인조적장도위1683.43 cM,각개련쇄군장도재36.11~131.48 cM지간,매개련쇄군적표기수재6~15개지간,표기간적평균거리위7.19 cM。결합F3재호북무한화양라배경하적주경고화총분지수감정결과,응용WinQTLCart 2.5연건채용복합구간작도법진행료QTL정위화유전효응분석。공검측도17개여주경고화총분지수상관적QTL위점,공헌솔재0.10%~10.22%지간,분포우8개련쇄군상。종합분석무한화양라배경적감정결과,획득중복일치적여주경고상관적6개 QTL,기중 qMHA061.1화 qMHA062.1위우련쇄군 LG06상 TC1A2~AHGS0153표기구간,공헌솔위5.49%~8.95%； qMHA061.2화 qMHA062.2위우 LG06상 AHGS1375~PM377표기구간,공헌솔위2.93%~5.83%； qMHA092.2화 qMHA091.1위우련쇄군 LG09상GM2839~EM87표기구간,공헌솔위0.53%~9.43%。
Peanut is an allotetraploid crop with a large genome. The construction of genetic linkage map and QTL mapping of related traits has little progress for peanut. In the present study, a genetic map consisting of 20 linkage groups was constructed with 234 SSR markers based on 2653 published SSR markers by using the F2population derived from the cross between Fuchuan Dahuasheng and ICG6375. The genetic map covers 1683.43 cM, and the length of each linkage group varies from 36.11 to 131.48 cM, the number of markers in each linkage group varies from 6 to 15, with an average distance of 7.19 cM. Combining with the data of main stem height and number of total branches of F3 population in the environments of Wuhan and Yangluo, we performed QTL mapping and genetic effects analysis of QTLs by software WinQTLCart 2.5 using CIM (Composite Interval Mapping) method. As a result, 17 QTLs related to main stem height and number of total branches on eight linkage groups were detected with contribution percentage of 0.10%–10.22%. Comparing the QTLs detected in the environments of Wuhan and Yangluo, qMHA061.1 andqMHA062.1were in the same linkage region of markers TC1A2–AHGS0153 of linkage group LG06 with con-tribution percentage of 5.49%–8.95%,qMHA061.2 andqMHA062.2were between the markers AHGS1375 and PM377 on linkage group LG06 with contribution ratio of 2.93%–5.83%,qMHA092.2 andqMHA091.1 were in the same linkage region of the mark-ers GM2839–EM87 in linkage group LG09 with contribution percentage of 0.53%–9.43%. The QTLs repeatedly detected are important for molecular breeding of peanut.