CAJ | 학술논문

以耐Cu性较强的烟草品种W38(Nicotiana tabacumcv.W38)和耐Cu性较弱的本氏烟(Nicotiana benthamiana)为试验材料,设置水培处理组即CK (Cu2+0 mg L–1, SA 0μmol L–1,即对照组)、T1(Cu2+4 mg L–1, SA 0μmol L–1)、T2(Cu2+4 mg L–1, SA 100μmol L–1)、T3(Cu2+4 mg L–1, SA 300μmol L–1)、T4(Cu2+4 mg L–1, SA 500μmol L–1),探讨SA对烟草生长特性及根、茎、叶不同器官中元素吸收规律的影响。结果表明,经过4 mg L–1Cu胁迫15 d后,2个品种的烟草生长均受到一定程度的抑制,不同器官中Cu含量均显著升高, K、Ca、Mg、Fe、Zn、B、Mn的吸收受到抑制,而在营养液中添加适宜浓度的SA能够有效缓解Cu胁迫对2个品种烟草根长、株高、鲜重的抑制作用,并降低烟草体内Cu含量。与T1处理组相比,本氏烟的根、茎、叶中Cu含量最大分别下降25.05%、39.78%和22.91%, W38的根、茎、叶中的Cu含量最大分别下降23.27%、37.30%和28.88%,并促进了营养元素K、Ca、Mg、Fe、Mn的吸收,但是对Zn、B元素的吸收影响并不明显。由此可知,高浓度的Cu胁迫会抑制烟草的生长及营养元素的吸收和运输,施加适宜浓度的外源水杨酸有利于营养元素的吸收和促进烟草的生长发育,在本试验浓度范围内施加300μmol L–1的水杨酸可显著缓解Cu胁迫对烟草的抑制作用。
이내Cu성교강적연초품충W38(Nicotiana tabacumcv.W38)화내Cu성교약적본씨연(Nicotiana benthamiana)위시험재료,설치수배처리조즉CK (Cu2+0 mg L–1, SA 0μmol L–1,즉대조조)、T1(Cu2+4 mg L–1, SA 0μmol L–1)、T2(Cu2+4 mg L–1, SA 100μmol L–1)、T3(Cu2+4 mg L–1, SA 300μmol L–1)、T4(Cu2+4 mg L–1, SA 500μmol L–1),탐토SA대연초생장특성급근、경、협불동기관중원소흡수규률적영향。결과표명,경과4 mg L–1Cu협박15 d후,2개품충적연초생장균수도일정정도적억제,불동기관중Cu함량균현저승고, K、Ca、Mg、Fe、Zn、B、Mn적흡수수도억제,이재영양액중첨가괄의농도적SA능구유효완해Cu협박대2개품충연초근장、주고、선중적억제작용,병강저연초체내Cu함량。여T1처리조상비,본씨연적근、경、협중Cu함량최대분별하강25.05%、39.78%화22.91%, W38적근、경、협중적Cu함량최대분별하강23.27%、37.30%화28.88%,병촉진료영양원소K、Ca、Mg、Fe、Mn적흡수,단시대Zn、B원소적흡수영향병불명현。유차가지,고농도적Cu협박회억제연초적생장급영양원소적흡수화운수,시가괄의농도적외원수양산유리우영양원소적흡수화촉진연초적생장발육,재본시험농도범위내시가300μmol L–1적수양산가현저완해Cu협박대연초적억제작용。
A hydroponic experiment was conducted to study the effects from treatmeats including CK ( Cu2+ 0 mg L–1, SA 0μmol L–1 as control), T1 (Cu2+ 4 mg L–1, SA 0μmol L–1), T2 (Cu2+ 4 mg L–1, SA 100μmol L–1), T3 (Cu2+ 4 mg L–1, SA 300μmol L–1), T4 (Cu2+ 4 mg L–1, SA 500μmol L–1) on growth characteristics, elements absorption and accumulation in two tobacco cultivars,N. tabacum cv.W38 (Cu resistant genotype) andN. benthamiana (Cu sensitive genotype) under copper stress. The result showed that the growth of two tobacco cultivars under 4 mg L–1Cu stress for 15 days was inhibited to some extent, meanwhile, the content of Cu in their organs was increased while the absorptions of K, Ca, Mg, Fe, Zn, B, and Mn were inhibited. The addition of exoge-nous SA could alleviate the inhibition of plant height, root length and fresh weight, and reduce Cu content of tobacco, but increase the absorption of nutritional elements K, Ca, Mg, Fe, and Mn. Compared with treatment T1, contents of Cu in root, stem and leaf were increased by 25.05%, 39.78%, 22.91% in N. benthamiana, and by 23.27%, 37.30%, 28.88% in N. tabacum cv. W38, respec-tively. In conclusion, appropriate concentration of SA promotes the growth and elements absorption in tobacco under copper stress. In this experiment, 300μmol L–1 SA could significantly alleviate copper inhibition to tobacco.